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We narrowed to 1,006 results for: RING

Showing: 601 - 640 of 1006 results

  1. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...Sample6, for advice on how to take an idea developed during graduate school and turn it into a biotech company...changed, but Sample6 still leverages the core engineering and scientific concepts to engineer bacteriophages...co-founders raised $6 million. The biggest challenge during this time was learning to communicate effectively...

  2. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...placing on your audience when you're in the midst of sharing your science - which can drive away even the most... them restate or summarize the concepts you’re covering. Don't show frustration if they're incorrect; ...as people discuss their experiences. If you’re hearing multiple people with similar misconceptions, it...

  3. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...polyclonal antibodies include any antibodies produced during the immune response.   Polyclonal antibodies...If your protein might become slightly denatured during your experimental protocol, using a polyclonal ... (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https://doi.org...

  4. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ... really, and what new questions does this paper bring up? We’ll sift through the paper to understand what...results in fewer eggs. However, the potentially staggering costs of such a treatment on top of the expense... Mary Gearing...

  5. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...within both the synthetic biology and genome engineering fields. We’ll walk you through how to apply this... Engler & Marillonet. Golden Gate and genome engineering In early 2011, the Bogdanove and Voytas groups... Mary Gearing...

  6. Working with Nuclear Receptors

    Type
    Blog Post
    ...mining tool, Transcriptomine. We are currently nearing the completion of an 18-month effort to overhaul...identifying plasmids in the research literature and acquiring them in a timely manner. Addgene represents an...an excellent solution to the problem of sharing plasmids, providing researchers with a one-stop shop for...

  8. Engaging with science and society at pgEd

    Type
    Blog Post
    ...disparities. But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists...ways of collaborating, and to organize events that bring different voices together to talk about genetics...running these events will be important both in preparing you for future work in these areas, and in helping...

  9. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ...characterized in an unrelated worm (C. elegans) [2]. Delivering dsRNA matching a specific mRNA sequence triggers...and silences its expression. More specifically, during the process of RNAi, an enzyme dubbed Dicer cuts...smaller dsRNA fragments are referred to as small interfering RNAs (siRNA) and they bind to a class of Argonaute...

  10. Lab Automation at Addgene

    Type
    Blog Post
    ...out your samples. This can be useful if you’re transferring samples from 96 wells plates onto agar q-trays...bacterial samples at the same time as opposed to preparing each sample individually. Enter the BIOROBOT! ...brushes the information onto the vials instead of requiring us to put a label on each tube by hand. We’ll ...

  12. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...produced when the candidate proteins’ interaction brings the pieces of the FP back together (Figure 1). ...interaction in vivo or in vitro, and can be used for measuring protein expression or localization in cells, or...yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. Lu et al. Plant J. 2010 Yuhai Cui...

  13. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...University. Some enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or... 2018). This means that off-target loci – in neighboring chromatin or throughout the genome – could be...that site. So, when DNMT1 methylates the genome during DNA replication, it doesn’t methylate sites blocked...

  14. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...create a successful science blog  5) Get creative – Bring your knacks into play Perhaps writing just isn’t...projects, you can set up a citizen science project. Answering the big science questions around climate change...guest blogger, Sarah Schmidt! Sarah Schmidt is engineering resistance against Fusarium wilt disease in bananas...

  15. Design Tips for Prime Editing

    Type
    Blog Post
    ...extension begins with a C base can cause unwanted base pairing (left), while pegRNAs that use other bases in this...the cell to use the edited strand as a template during DNA repair. Test multiple nick sites, starting ... linkers that minimize unwanted intra-RNA base pairing with the primer binding site (Nelson et al., 2022...

  16. Hot Plasmids February 2024

    Type
    Blog Post
    ...here!   Zhang, Z., et al. (2023). Efficient engineering of human and mouse primary cells using peptide-assisted...effective at breaching the blood-brain barrier and delivering a transgene, with neuron bias observed in young...the Gradinaru lab, AAV1-X1, was developed by transferring the 7-mer peptide region from variable region...

  17. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...publications in the fields of research they support. Monitoring these types of accounts can help you find papers...papers later. Pro tip Learn more about authors by exploring author pages (for example, Melina Fan’s author... Instead, you create your own research feed by entering keywords, concepts, journals, and/or papers of...

  18. 6 Tips for Grant Writing

    Type
    Blog Post
    ...approach that problem in a novel way. Finally, engineering new applications to solve problems will provide...’s a good idea to move around to different labs during your career; you learn new techniques and interact...get feedback from the funding body through their scoring system or meeting with their grant submission panel...

  19. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...the moderator should be taking good notes and discovering interesting stories, riffs or opinions that each... a fun discussion. People are much better at remembering these things on a relaxed phone call than when...The idea is to ask questions in a directed way to bring out these interesting stories and remind the panelists...

  20. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ... unable to participate in genetic recombination during meiosis. Also, flies are not viable if they are...know is that males do not recombine chromosomes during meiosis. This can sometimes be a handy feature ...Like Addgene, the Drosophila community is highly sharing-oriented, and there are many resources to help ...

  21. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...separate proteins should be useful for monitoring cellular events occurring at varied time scales.  Table 1:...the FP timers, add an entire new dimension to monitoring localization; using FP timers, researchers can...

  22. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...more gRNAs must be used along the same chromosome. During DNA repair, there is a chance that the two breakpoints...Harnessing type I CRISPR–Cas systems for genome engineering in human cells. Nature Biotechnology, 37(12),...CRISPR-Cas Systems INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Additional resources...

  23. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...powerful tool, it was promising enough to keep exploring and to try developing a variant with an enhanced...variants of MagLOV will be possible with other engineering approaches and that magnetosensitive tools can...often posting updates on social media) and freely sharing code, raw data, and more. The team shares results...

  24. Pooled Library Amplifications

    Type
    Blog Post
    ... library is maintained and reduce recombination during the amplification process. In liquid culture, all...recombination. If you do see recombination, we recommend ensuring the cells used are meant for repetitive sequences...Addgene blog Genome-Wide Screening Using CRISPR Preparing Pooled Libraries NGS Quality Control for Pooled...

  25. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...mass bleaching, the loss of their symbiotic algae, during heat stress. For other organisms, like the sea ... Wistrand-Yuen, P., … Forster, A. C. (2018). Engineering a palette of eukaryotic chromoproteins for bacterial...bacterial synthetic biology. Journal of Biological Engineering, 12(1). https://doi.org/10.1186/s13036-018-0100...

  26. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...an industry postdoc is something that you’re considering. Like anything, there are pros and cons to postdocs...number of steps, decisions and people involved is staggering. The process is complicated. If you’re interested...all about teamwork. A single individual could not bring a drug to the market, so people in industry have...

  27. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...media with supplements such as magnesium salts, buffering agents, and/or supplying additional carbon sources... yield. Start with a single, fresh colony Sub-culturing directly from a frozen glycerol stock or agar ...harvesting and miniprepping at various time points. Measuring the optical density of the culture can indicate...

  28. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...blastn program uses a smaller word size and lower scoring penalties for mismatches and gaps in the alignment...blastp algorithm parameters. We use the default scoring matrix BLOSUM62, but you may want to check the ... multiple protein sequences, particularly for comparing different isoforms. In addition to our favorites...

  29. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... It may be helpful to incorporate regular feeds during this incubation time to maintain cell viability...consider increasing the number of washes performed during your harvest. Validation Recombinant antibodies...temperature shifts. Of course, we are also cautious during the aliquoting and storage process to prevent dropped...

  30. An Introduction to Adenovirus

    Type
    Blog Post
    ...inhibition of the innate immune system; and reconfiguring of the nuclear compartment, gene expression,...’ functions are rescued by helper plasmids used during production and/or by genes present in the HEK293T...license.   The promise of adenoviral vectors This brings us to the final topic, which is that natural AdVs...

  31. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...for distribution. If your experiment involves delivering a calcium biosensor using AAV, you might decide...viral preps instead of plasmids? The available filtering options now change dynamically based on the type...target organism. Clicking through to RNA Editing brings us to a page with a basic overview and figure, ...

  32. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ... the genome, they could promote oncogenesis by altering local gene expression. Self-inactivating (SIN)... PubMed Central PMCID: PMC4486245. 5. Patient monitoring and follow-up in lentiviral clinical trials. ... Mary Gearing...

  33. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...up colony PCR reactions is nearly identical to preparing a standard PCR reaction: combine template, primers...other colony PCR tips are highlighted below. Preparing template: Pick a single colony with a sterile ...to the PCR reaction. The bacteria will be lysed during the initial heating step of the PCR reaction. A...

  34. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...requirements. Cpf1’s preferred PAM is 5’-TTN, differing from that of Cas9 (3’-NGG) in both genomic location...repair templates should remove the PAM sequence. Comparing Cpf1 and Cas9 on-target and off-target efficiency... Mary Gearing...

  35. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ... training, mentoring and coaching services, including Smartegrants, to help those entering the Grants ...Twitter Chat, and then our grant training and mentoring. How do I become a grant professional? Employment...

  36. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...first non-Cas9 CRISPR protein adapted for genome engineering was Cas12a (formerly Cpf1), a nuclease that generates...comment below. Note: Jennifer Tsang and Mary Gearing contributed to the update of this post. References... genome engineering...

  37. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ... the promiscuous cleavage and degradation of neighboring ssRNA and ssDNA by Cas13 and Cas12a, respectively....org/10.1038/s41587-020-0513-4 Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, Palefsky JM, Doudna JA...Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM, Freije CA, Myhrvold C, Bhattacharyya RP, Livny...

  38. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal circuits. Epitope ...tags, low FP expression may be insufficient for monitoring a weakly expressed POI. Spaghetti Monster - A...fluorescent protein structure and color variants." Cold Spring Harbor Protocols 2009.12 (2009): pdb-top63. PubMed...

  39. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...subpopulations of cells, you’ll soon find yourself encountering flow data in the literature. Data reported from...of flow cytometry is to use increasingly more stringent markers to identify your specific cell population...are used to separate single cells from doublets, ignoring anything not within the gate of the first plot...

  40. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ... resistance can be the result of one bacterium sharing genes with another, an accidental transmission ...means to defend themselves from these naturally occurring antibiotics. When we use antibiotics to treat ...prescribed to them This may seem counter-intuitive considering the other three scenarios, but a short treatment...
Showing: 601 - 640 of 1006 results