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We narrowed to 974 results for: lat

Showing: 601 - 640 of 974 results

  1. Hot Plasmids - October 2022

    Type
    Blog Post
    ...-2P. Using a custom, high-throughput screening platform to apply saturating mutagenesis on 21 positions...'s more than 50 antibodies target neuroscience-related proteins, but we recently added several broadly...here!   Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific for human c-...

  3. Plasmids 101: Antibiotic Resistance Genes

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    Blog Post
    ...aminoglycoside Binds 30S ribosomal subunit; causes mis-translation Bactericidal 50-100 ug/mL Spectinomycin aminoglycoside...months. Ampicillin breaks down especially fast and plates should be used witin 1 month for optimal efficiency... them to media hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic...

  4. Plasmids 101: TOPO Cloning

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    Blog Post
    ... Pre-warming your antibiotic-containing plate prior to plating your transformation may allow you to see...TOPO technique specifically uses Vaccinia virus-isolated topoisomerase I as this enzyme recognizes the ...

  5. Identifying Sequence Elements with SnapGene's Feature Database

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    Blog Post
    ...software tools available to biomedical researchers manipulating DNA sequences on a daily basis, many found these...control elements Coding sequence features are relatively straightforward to define, but for control elements...fluorescent proteins, which often come in closely related versions that have different properties. To prevent...

  6. Viral Vectors 101: Transductions

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    Blog Post
    ...cargo, they can’t reproduce more functional viruses later on. These built-in safety features provide extra...integration resulting from transduction typically plateaus within a few days to a few weeks, depending on...host nucleus, is transcribed into RNA, and is translated into protein.   Is there an easier way? In...

  8. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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    Blog Post
    ...launched this pilot in 2019 as an open science platform for scientists using Addgene AAV viral preps to...all of your data prepared. All of these questions relate to factors that you should have already recorded...reproducible research. Share your data on AAV data hub! Related resources on the Addgene blog Important Considerations...

  9. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post
    ...protein could cleave DNA in vitro, directed by phosphorylated DNA guides. Unfortunately, Swarts et al. were...able to mediate homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication...in GC-rich regions. DNA guides NgAgo uses 5’ phosphorylated DNA guides (so called “gDNAs”) rather than ...

  10. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...plasmid” derived as a hybrid of "cytoplasm" and "id" (Latin for 'it'), as “a generic term for any extrachromsomal...integrated into the chromosome” was proposed a few years later by Élie Jacob and François Wollman and became the...PMCID: PMC427208. Novick, R P et al. “Uniform Nomenclature for Bacterial Plasmids: A Proposal.”Bacteriological...

  12. Another Pathway into Cells: iTOP

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    Blog Post
    ... Primary cells recapitulate the natural biology of a cell type of interest better than immortalized lines...to describe this method of protein transduction. Later studies showed that iTOP functions through macropinocytosis... sorted into single wells to generate clonal populations. After iTOP treatment, both primary (hESCs) and...

  13. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...ways to apply fluorescent proteins everyday.  The late Michael Davidson and Roger Tsien played enormous...enzyme active site and shutting off the enzyme. Later exposure to cyan light stopped Dronpa oligomerization...Fluorescent Light-inducible Proteins, or FLiPs.  Xin later optimized these first generation FLiPs by mutating...

  14. Creating accessible biology activities in schools with BioBits

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    Blog Post
    ...expensive) the different machines are, and how many regulations are in place to keep everyone safe. None of this...fluorescent protein through transcription and translation. Unfortunately, having live cells in a classroom...hands-on educational activities using the BioBits platform, please get in touch with us! We’ll work with ...

  15. 25 Work From Home Tips for Scientists

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    Blog Post
    ...at Addgene began working from home last week to flatten the curve, but working remotely can be more difficult...this period, check out these tips and resources collated by Addgenies to help you keep calm and science...local businesses (ex: purchasing a gift card to use later or ordering take-out) or volunteering to foster ...

  16. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    Blog Post
    ...targeting the genes responsible for both early onset and late onset AD. In this study, the group showed that CRISPR...Alzheimer’s disease, as well as those that play into late onset AD (5).     CRISPR/Cas9 corrects Alzheimer...CRISPR to disease correction in adult stem cells isolated from two patients with cystic fibrosis. They were...

  17. How to Lead a Great Meeting

    Type
    Blog Post
    ...communicate outcomes to the rest of their team. Here's a template for project meetings. Team Meetings: For standing...members still have their voices heard. Here's a template for team meetings. One-on-Ones: Managers should... agenda template...

  18. Resume Writing for Non-academic Science Careers

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    Blog Post
    ...people choose a reversed chronological style (the latest to the earliest) or a functional one. Pick one ...when the research topic you have done is closely related to the job you are applying for, in which case ...in Experimental Medicine. She is the Community Relations Director for Curiosity Collider (hyperlink https...

  19. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...be covering this ever-versatile fluorophore in a later post, so stay tuned! Transient transfection and ...transfection A stable transfection is used to create a population of cells that have fully and successfully incorporated... COS-1, 293HEK Inhibits termination step of translation 2-10 ug/mL G418/Geneticin neo HeLa, NIH3T3,...

  20. From Lab Bench to Leadership: Transitioning into Management as a PhD

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    Blog Post
    ...lab or pivot to a managerial role. For those contemplating the leap into management, the journey can be...being a great scientist doesn’t automatically translate to being an effective manager. Mismanaging (no... Kim Scott Courses: Leadership programs on platforms like Coursera, LinkedIn Learning, or edX. Podcasts...

  21. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...hepatitis virus post‐transcriptional regulatory element; sequence that stimulates the expression of transgenes...-retroviral plasmids have similar names? The nomenclature of the plasmid backbone is meant to be descriptive...

  22. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    Blog Post
    ..., integrated reporter for assaying CRISPR/Cas-stimulated HDR activity) The Madison lab created SRIRACCHA...Joung JK (2014) CRISPR-Cas systems for editing, regulating and targeting genomes. Nature Biotechnology 32...integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. Journal of Biological...

  23. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...contained cold tolerant chaperones (the Cpn 10/60 isolated from Oleispira antarctica, and the P. haloplanktis...psychrophilic systems as well as engineering and regulating psychrophilic recombinant proteins.  To infinity...2019 All-India iGEM Meet-up, which served as a platform for all the Indian iGEM teams to interact with...

  25. Viral Vectors 101: Viruses as Biological Tools

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    Blog Post
    ...scientific and medical tools. As scientists, we can manipulate what this cargo is and where it goes, turning...mutagenesis in the host. Adenoviruses can deliver relatively large cargo inserts (>8 kb). Adeno-associated...their low immunogenicity, which means they do not stimulate a strong immune response when introduced into ...

  26. Building and Validating Recombinant Antibodies: A Case Study

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    Blog Post
    ...plasmids for expression of mAbs, the Trimmer lab isolated RNA from cryopreserved hybridomas, immortalized...then used to generate cDNA, which  was used as a template for PCR amplification with a degenerate primer...grown in tissue culture, they were also able to isolate RNA from non-recoverable hybridoma stocks, without...

  27. Hot Plasmids - February 2022

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    Blog Post
    ...miniature CRISPR-Cas system for mammalian genome regulation and editing Anti-FLAG Frankenbodies for modular... genes, researchers at the Genetic Pertubation Platform at the Broad Institute have expanded the genetic...miniature CRISPR-Cas system for mammalian genome regulation and editing by: Rachel Leeson The large size ...

  28. 7 Tips to Secure a STEAM Internship This Summer

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    Blog Post
    ...certain to choose your recommenders based on your relationships to the person as well as their current sphere...just as you would for an in-person interview. Formulate and practice a set of common questions with a ...academic resources among economically disadvantaged populations. Roo is the co-founder of STEAMid.       Rose...

  29. CasPEDIA: A Functional Classification of Cas Enzymes

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    Blog Post
    ...sequence (NGG, etc.) as well as their orientation relative to the gRNA (5’ or 3’). In some cases, there is... used by Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got...to browse entries by the current phylogenetic nomenclature and discover orthologs with similar properties...

  30. A Deep Dive into BioBuilder

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    Blog Post
    ...cellular extracts to perform transcription and translation in vitro, and the new BioBuilder module allows... mentors, and provides a nationally recognized platform in March to exhibit projects at any stage of completion...breakdown, production of fire-resistant housing insulation, and cleaning up salt runoff from winter icing.BioBuilderClub...

  31. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    ...effort to track down every last one of those gene regulatory proteins and the places in the genome where they...closely together on several projects and this is one related to gene expression. One goal of the ENCODE project...resistance to neomycin so that you can select the population of cells with the tagged transcription factor...

  32. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...community. As a result, I have occasionally pondered translating my research into a startup company. However, ...effort in preparation and practice is directly related to success presenting." A journey from graduate...LabSpaces Blog Find a Buisness Plan Competition Good Relations - PR for the Biotech Business - Posted on Nature.com...

  33. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ... the cell with a mutant template and fooling the cell into using that template for repair. By using these...the two ends back together or using a homologous template provided by the sister chromosome. As cells take...

  34. CRISPRainbow and Genome Visualization

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    Blog Post
    ...distances of 2 and 75 Mbp, with the calculated fluorescent distances correlating with the previously established...simultaneously, they could also monitor the spatial relationship of the two genes over time, at a range of distances...

  36. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...string together all the words that I could remember related to this paper and/or biology in general until I... turn your slide deck into a chalk talk. Your translator doesn’t know the term “diving board” so you end...or talk extremely quickly. You realize, much too late, that you labeled your gene of interest incorrectly...

  37. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...’t just for DNA anymore! Cas enzymes have been isolated which target and cleave RNA through the same mechanism...and 2). cell toxicity. Cas7-11 does not display collateral RNA cleavage and is significantly less toxic ...mammalian cells than Cas13 (likely due to the lack of collateral cleavage). You can read about Cas7-11 in our ...

  38. Antibodies 101: Selecting the Right Antibody

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    Blog Post
    ...pathway is regulated. You’ve read all the background papers you could get your hands on, formulated a hypothesis...immunoprecipitation-mass spectrometry strategies that isolate an antibody’s immune complexes and confirm interacting...

  39. What's New in CRISPR - November 2021

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    Blog Post
    ...available at Addgene. If you want to read more content related to CRISPR technology, subscribe to our CRISPR blog... plasmids Enhanced prime editing systems by manipulating MMR machinery In their Cell paper, the Liu Lab...other prime editing tools, the desired edits are templated by the 3′ extension of the pegRNAs. PEDAR is capable...

  40. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...amplitude, wavelength sensitivity, and off-kinetics correlated with the model’s predictions.  Three top performers...reach the VTA. Mice were then allowed to “self-stimulate” their VTA dopaminergic neurons by triggering ...H134R) expressing mice did not have this self-stimulation response. This result showed that ChRger2 stronger...
Showing: 601 - 640 of 974 results