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  1. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches to presenting...Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian...

  2. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...involving multiple scientists, create and stick to timelines Be a mentor in a formal program Start something... publications like FierceBiotech to “learn the business” Attend events with local networking and trade...

  4. What's New in CRISPR - December 2019

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    Blog Post
    ...Yachie’s lab recently developed a base editor that combines the abilities of C-to-T and A-to-G base editors...TadA) to a catalytically impaired Streptococcus pyogenes Cas9. They called this editor Target-ACE (adenine...

  5. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ... dynamics can be undertaken using available GFP lines. Like many great scientific discoveries, this one...for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et...

  6. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...you experiments, keep track of upcoming grant deadlines, and sync everything to your calendar. Ditch the...Tasks to create lists of your upcoming tasks, set deadlines, and arrange automatic email reminders. These ...

  12. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...gene. Vital component for expression vectors: determines which cell types the gene is expressed in and... flexibility, versatility, safety, and cost-effectiveness enable molecular biologists to broadly utilize...

  13. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ... Rare Disorders (NORD). NORD raises money and awareness for rare disease and helps patients find the correct...an organization dedicated to raising funds and awareness of FOP. Please consider running with us or making...

  14. Addgene at the ASM Microbe 2016 Conference

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    Blog Post
    ...raffled off one of our highly sought-after "nice genes…" tshirts, and the lucky winner was Shagun Sharma...to be had at ASM Microbe as well as the serious business of science. Two symposia on Sunday afternoon were...

  15. Management for Scientists: Managing vs. Leading

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    Blog Post
    ...any goal or finishing any project. Doers are the ones who do the work Doers focus on understanding what...Marshall Goldsmith, with Mark Reiter Harvard Business Review 10 Must Reads: On Leadership (Articles ...

  16. Choosing a Good Mentor for Scientists

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    Blog Post
    ...postdocs do to ensure the advisors they choose will be ones that help them succeed after the training period... familiarity and confidentiality to ensure more honest answers. Ask people who work in the labs next door...

  17. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...derived were tested for specificity in knock-out lines or animals.  Addgene will continue to add more antibodies...antibodies, for neuroscience research and other disciplines, over time. The resource may also expand to include...

  18. RUBY-Red Siliques

    Type
    Blog Post
    ...fluorescent proteins. Selection using resistance genes usually involves killing the untransformed seedlings...then green offspring can be selected instead of red ones. Other innovative uses of RUBY are possible, for...

  19. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...transduction methods. Some ‘difficult to transfect’ cells lines are amenable to transduction, making it a logical...Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene...

  20. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...partially reprogrammed and re-expressed fibroblast genes. Most cells ended up taking the ‘dead-end’ path....CellTag is a combinatory cell-indexing tool that combines scRNA-seq and lineage tracking to track the dynamics...

  22. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...with different properties (for example, overall brightness or on/off kinetics) that makes them suitable ...pAAV.hSyn-FLEX.iGABASnFR) has good membrane localization and brightness. The F102G variant (pAAV.hSyn-FLEX.iGABASnFR....

  24. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ... and genomic DNA in a variety of mammalian cell lines, with an efficiency similar to that of Cas9. Like...certain guide sequences - using 5 guides/gene for 10 genes, they did not observe differences in cleavage efficiency...

  27. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...my guess is that people will not be exchanging business cards for awhile or ever so your LinkedIn profile...She supported me and answered my questions and honestly, she was the only person who really understood...

  28. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ... know that science usually doesn’t follow our timelines! Prepare a research plan to set yourself up for...applying to a structured program, they likely have guidelines to consult on this topic.   Etiquette...

  29. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...experimental model. In fact, approximately 25% of yeast genes have a human counterpart, enabling study of human-relevant...the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation...

  30. 25 Work From Home Tips for Scientists

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    Blog Post
    ... help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace practice...19. You can also give back by supporting local businesses (ex: purchasing a gift card to use later or ordering...

  31. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...going forward. This is not just a question of being honest and straightforward, it’s an important part of ... various different versions of checklists and guidelines to help authors understand what they are expected...

  32. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...the gRNA target site. In addition to increased brightness, they also found that the signal to noise ratio... observing low-copy genomic loci or single copy genes. If you want to visualize genomic loci brightly,...

  33. Cultivating Community Science at BosLab

    Type
    Blog Post
    ... with audiovisual media, software engineering, business consulting, mathematics, and more. That’s right...BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge with the community...

  34. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...smartphone to help me with my research in terms of preparedness, organization, and sharing data. Here are five...Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State University. He works with gene expression...

  36. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...performed using traditional 2D assays with immune cell lines or peripheral blood mononuclear cells (PBMC) in .... P., Herskovitz, J., Goletz, T. J., Zhou, L., Moxness, M., Flynn, G. C., Narhi, L. O., & Jawa, V. (2016...

  37. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...disrupt target gene sequences (thus inactivating genes), while breaks repaired by homology directed repair... which sequence replacement holds some kind of fitness advantage but error-prone repair of the gene would...

  38. Components of CRISPR/Cas9

    Type
    Blog Post
    ...Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant...sites with the aid of two RNAs: the crRNA which defines the genomic target for Cas9, and the tracrRNA which...
Showing: 601 - 640 of 905 results