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We narrowed to 963 results for: APP

Showing: 301 - 320 of 963 results

  1. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...generation of various flux imbalances. The conventional approach has typically been to either systematically delete...networks and multiplex gene control. However, these approaches are often met with the challenges of regulatory... possible. From a design perspective, this is appealing since the RNA scaffold modules are comparatively... multiplex control via scRNA design is readily apparent in metabolic engineering. The authors elegantly...control of gene expression. This strategy can be applied to effectively change metabolic flux. The dCas9...

  2. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...IVT sgRNA) approach (Dewari et al. 2018 and Figure 2). Not only is the cr:tracrRNA approach highly efficient...biochemical tasks in the cell. One of the most popular approaches to study protein interactions is the pull-down...disrupts protein function, an N-terminus tagging approach should be pursued, however, clonal lines must ...me at [email protected]. Keep pipetting and happy genome editing! Pooran Dewari is a postdoc in Steve...optimizing CRISPR/Cas9 genome-editing methods and applying them to study brain tumour biology. When not pipetting...

  3. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...axial dimension that will appear in focus). This means that the images will appear sharp without interference...excite a molecule of GFP. Because red light has approximately half the energy of blue light, two photons of...density of red-photons be establish for this to happen.Using red light has a second advantage: red light ...objects lying within the diffraction limit will appear as one object in the final image. Over the past...of light – think of a single GFP molecule – will appear as a fuzzy 200-300 nm spot when imaged. Super-resolution...

  4. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...interactions. Beyond these common applications, researchers are finding new ways to apply fluorescent proteins everyday...Zhou and Fatima Enam. There were many excellent applicants but Xin and Fatima stood out for their creativity...Light-inducible Proteins (FLiPs) Fluorescent proteins can be applied in a long list of creative ways. In her graduate...utility of these FLiPs in a variety of research applications and particularly notes their ability to be precisely...

  5. Hot Plasmids - January 2023

    Type
    Blog Post
    ...have high levels of off-target effects, both of happen with Cas13. CRISPR-Csm degrades both nuclear and...antibodies may just not perform as well in one application as another, so it is always best to have options...for mEGFP-HRas (from Plasmid 18662). See the Applications section on the Anti-GFP [N86/8R] page for more...fluorescent proteins (FPs) necessary for many of these applications have lagged. Fixation, chemical staining, and...well-suited for the demands of advanced microscopy applications such as super resolution, electron, and expansion...

  6. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ... of applications with the potential to save lives. While most scientists recognize and appreciate the ... Yamanaka himself, during his keynote address, appeared almost incredulous that the field has exploded...daunting because they have no clear cut answers, I applaud the stem cell community for addressing these important...but they are also the reason this research is happening. Stem cells scientists have certainly not lost...

  7. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...altering its function). HDR is crucial for certain applications, for example, correcting the allele that causes...this question led us to develop a new, rational approach to improve the efficiency of sequence replacement...introducing a cut, with residence times on DNA of approximately 6 hours. To our surprise, we measured identical...region allowed sequence replacement rates of approximately 1%. This is by no means a large number, but ... order of magnitude greater than transfection approaches. Moreover, the cost of reagents is relatively...

  8. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...together a grant application? Are you familiar with the publishing or patent application processes? How ...How can you stand out among all the candidates applying for the position?   Here are a few things to consider...makes sense for your skills, and the job you are applying for. Also remember that a resume is typically ...have done is closely related to the job you are applying for, in which case talk about your research, as...

  9. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...multicolored nano-lanterns for use in various applications, including optogenetics, biosensors, and fusion...furimazine. In addition to the previously described applications, the enhanced signal of eNL allows their use...characterizing Nano-lanterns and eNL for many different applications. For long-term imaging, especially where photobleaching...concern, these constructs come in very handy. This approach is especially suited to complement optogenetic...Therefore, especially thick samples may not be appropriate for use with Nano-lantern technology. Multicolor...

  10. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ... by the Dueber Lab at Berkeley, provides a new approach for generating novel genetic variants in bacteria...the key components of EvolvR and its potential applications. Challenges of existing mutagenesis methods...exist, but there are a few drawbacks with these approaches that makes it challenging for users to achieve...is capable of mutagenesis in E. Coli, but this approach requires special bacterial growth conditions in...more than one mutation near the target site. Applications of EvolvR The Dueber Lab used EvolvR to discover...

  11. Antibodies 101: ChIP

    Type
    Blog Post
    ...next-gen sequencing. ChIP is an incredibly useful application for exploring these interactions, and can give...including epigenetic modifications. But it's not an approach for the faint of heart!  The Process ChIP can...preferred method, enzymatic digestion can be appropriate when your protein of interest is a low abundance... upfront investment than many other antibody applications, this investment can well be worth it once you... idea to add "patience and an extra-methodical approach" to the top of your protocols when embarking on...

  12. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...disciplines. In biology, innovative new imaging approaches are constantly being developed which combine ...biological protocols and revolutionary physical approaches. However, with each new technical achievement... greatly increases accessibility. Through this approach, the Planktoscope, a portable device for studying...on-going creativity with the construction plans. Appropriate open-source licenses for hardware (e.g. CERN ...optical setups. References Joel T. Collins, Joe Knapper, Julian Stirling, et. al, "Robotic microscopy for...

  13. Clear and Accessible Writing

    Type
    Blog Post
    ...principles we used in our daily content writing applied to alt text. We also were aware of the need for...they hoping to get from it? The image in Figure 1 appears on our protocol page for AAV Purification by Iodixanol...a lot of jargon and have a technical, detailed approach. Other times, it is more helpful to use plain ...start small with alt text or immediately dive into applying these four steps elsewhere, engaging in this practice...from posters to papers.  We hope this helps, and happy writing!   Resources Additional resources on the...

  14. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...advances and how some of these variants may be appropriate for your specific research question. Considerations...off-target activity, which may be undesirable in many applications. At the time of our first blog post on choosing...Using a combined random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9, which...enzymes may better suit specific experimental approaches. One example is CasX, characterized by the Oakes...While no enzyme is perfect for all experimental approaches, knowledge of both the advantages and limitations...

  15. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...some reporters are better suited for particular applications. Read on to learn more about each reporter or...sensitive than HRP RANbody detection, it is approximately as sensitive as detecting the same antigen with...less sensitive than HRP RANbody detection but approximately as sensitive as antigen detection with a standard...detected with a labeled anti-HA or -MYC antibody, approximately as sensitive as detecting antigen with a standard...Detection with an anti-Chicken IgY antibody is approximately as sensitive as detecting antigen with standard...

  16. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...brains. However, it is not known why or how this happens. Therefore, I set out to study how protein turnover...protein turnover in situ in neurons. The classic approach for studying protein turnover is radioactive pulse-chase...immunocytochemistry is perhaps the most common approach. With a good and specific antibody and the proper...proteins with FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid... observed with fluorescence microscopy. This application of AHA labeling is known as fluorescence noncanonical...

  17. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...two posts in this series described a practical approach to selecting a bright fluorescent protein and ...aggregates. This property can be tolerated in some applications (e.g. labeling of cells or tracking promotor...promotor activity), but it is problematic in applications in which the fluorescent protein is used as an inert...cells. To understand how well fluorescent proteins approach this ideal situation, cellular assays have been...can be used in the OSER assay including CytERM-mKOkappa, CytERM-mTurquoise2, and CytERM-dTomato. When...

  18. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...DNA-binding proteins to label certain loci, but this approach is not scalable for every locus...unlike CRISPR... 9 and 13. They next turned their attention to mapping pairs of intrachromosomal loci. The technique successfully...authors’ knowledge, this work represents the first mapping of intrachromosomal loci, a major benchmark in ...-MS2-PP7 Despite the success of Ma et al.’s approach, fluorescently labeled Cas9’s come with a number...up fluorescent CRISPR labeling, they took a new approach: labeling the gRNA itself. In their CRISPRainbow...

  19. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...regions, both through genetic and proteolytic approaches. This comes in handy when, for example, you want... as secondary antibodies, have much broader applicability. It is fairly easy to find these in a Fab fragment...pre-form primary-secondary antibody complexes before applying them to samples, such as when performing IHC in...can be used as a block in similar scenarios. By applying unconjugated Fab fragments that are the same species...fragments described above can be used for similar applications and have similar advantages, though with some...

  20. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...engineering fields. We’ll walk you through how to apply this precise and easy-to-use system to your cloning...designed correctly, the recognition sites do not appear in the final construct, allowing for precise, scarless...are eliminated by digestion/ligation and do not appear in the final construct. The destination vector ...adapted Golden Gate cloning for inserting the appropriate oligonucleotides specifying a gRNA target sequence...different combinations, or other new and exciting applications, this system is an incredibly powerful tool ...
Showing: 301 - 320 of 963 results