We narrowed to 666 results for: CHI;

...establish.These funding decisions will have far-reaching consequences throughout the scientific community... can turn to Addgene as a trusted partner for archiving their biological materials during funding transitions...

... Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're thinking about hosting multiple plasmids...incompatible. 4. RNA Extraction Without A Kit by Leah Schiesow We added to our protocol collection this year...

...Making the Right Connection by Joachim Goedhart Guest blogger Joachim Goedhart shares some strategies...

...contamination, but consider flow cytometers. These fluidic machines handle biological samples and are cleaned between...between each use. You wouldn’t throw away your FACS machine after a single use. So how about washing and reusing...

...Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes plasmids designed for...specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory capacities of ...

... by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and Harvard Medical School. Addgene ...hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical School. His lab ...

... in epitope tags? Others may be interested in attaching epitope tags to their genes of interest, allowing... developed in the labs of Anne Robinson, Eishi Noguchi, and Melissa Moore, to name a few. Have you used...

...materials that may be useful to your research. But searching our website also allows for filtering for materials...editor deposited by Profluent Bio, maintains Cas9 architecture, but is almost two hundred mutations away from...

... Delivery of AAV into the brain can usually be achieved by a directed brain injection, but some serotypes...types. Specific transgene expression can be also achieved in nontransgenic animals by using cis regulatory...

...shortened the T-DNA borders to 25 bases each. By switching the E. coli origin of replication and codon-optimizing...codon-optimizing the Agrobacterium ORI, they achieved much higher DNA yield and transformation efficiency in...

...been adapted to fluorescently label a sequence matching its gRNA, facilitating the live study of chromatin...under lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the...

...fluorescent proteins are, which makes mixing and matching a relatively easy proposition, explained Michelle...backbone and making fusions, see this blog post from Joachim Goedhart: Fluorescent Proteins 101: GFP Fusion ...

...-infrared range, and that’s exactly what iRFPs achieved. Those proteins allowed a signal-to-background...conventional FPs. In addition, PA FPs can improve the achievable signal-to-background ratio, thus, allowing higher...

...seeing early results that suggest our content is reaching more people who need it!  After a few months, ...followed with great discussion and questions. I achieved my goal of helping everyone understand the importance...

...for plasmids, so that you can spend less time researching the basics and spend more time developing cleverly...replication within a plasmid by recruiting replication machinery proteins Antibiotic Resistance Gene Allows for...

...biomedical researchers and ready access to stably archived large-scale datasets by computational biologists...interest using a simple keyword search. For example, searching the term "breast cancer" returns 40 screens performed...

... tool to detect and image DA in live animals (Patriarchi et al., 2018). Similar to calcium and glutamate...PMID: 4403305. PubMed Central PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging ...

...a higher signal-to-noise ratio with a lower photobleaching rate. Given the power of SunTag, they attempted... ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule tracking...

...standards. Bricking science reminded us of our inner children who have no preconceived obstacles, optimistically...outside the box, be creative, and put your inner child to work. If you have an odd or quirky lab story ...

...been requested more than 100 times each, thereby achieving the coveted “blue flame” status. We’re proud to...scientific research. As Maria Castro of University of Michigan says, “Addgene has transformed the way in which...