...like AAV. In addition to the mammalian option described below, plasmids for making polycistronic gRNAs...oligonucleotide assembly.  Unlike some of the plasmids described above, this vector does not contain Cas9, so you... are similar to the Csy4 polycistronic system described above, except that they use an endogenous nuclease...

...AnoeskaMoosdijk @Saskia_deMan @SILS_UvA @addgene #SciArt #fluorescence #LCAM #DeckTheLab pic.twitter.com/pYBL3aLK4f...from lab gloves. The tree was adorned with a bioluminescent tree topper, tube “lights,” and autoclave tape...

...article describing a sucrose supplementation strategy to improve yield. In addition, as described in this...

...your experiment* Functional data, if the report describes a functional vector such as a channelrhodopsin...site as well as the target region, if the report describes a retrograde serotype An abstract that summarizes...

...Fessenden at Brigham and Women's Hospital. This post describes how to target and use FRET flurophores for a variety...phenomena and about how fluorophores other than fluorescent proteins can be incredibly useful in FRET experiments...

...researchers at Japan’s Hiroshima University who described their new tool in Scientific Reports in June. ... gap. As Sakuma and colleague Takashi Yamamoto describe in their recent study, the construction system...

...service features 210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is...Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate color! They'...

...activated and can cleave fluorescent probes of dsDNA and RNA in trans. Fluorescent signal indicates the presence...

...paper thin and very portable microscope. Less fluorescence admittedly, but still so beautiful! Enabling...agar art have a look at our Microbiology and Fluorescent Protein Resources and get started!  I learned...

...Jessica Sacher from the University of Alberta and describes a technique you can use to find phage proteins...molecular biology tool, P1 phage. 2) The post describes an ingenious but simple technique to screen for...

...process When I first saw the comprehensive job description for this co-op, I was immediately interested ...had stood out to me because it gave me a full description of the tasks I would be responsible for, in comparison...

... Michael Davidson Fluorescent Protein collection, a ‘One-Stop Shop’ for Fluorescent Proteins, and plasmids...

... reagents from 20 contributors, including 61 fluorescent probes, representing imaging from 47 different...the Addgene blog Antibodies 101: Multiplex Immunofluorescence Negative Can Be Positive: Open AAV Data with...

...Cas9 and a donor vector to create knock-ins of fluorescent proteins, fusion proteins, or transcriptional...interest and on either side of the cargo (ex: fluorescent protein gene) on the donor vector. Therefore,...

...Two new human lentiviral CRISPR library systems described in companion papers in Science in December were...Additionally, a concurrent Nature Biotechnology article describes the development of a mouse lentiviral CRISPR library...

... optogenetic tool (CsChrimson) attached to a fluorescent indicator (tdTomato), as the effector. They also...expression in flies Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular...

...fascinating and fun look at her outreach work with fluorescent microbes. Check out her animation about the Hawaiian...repository (like these new Rainbow Bacterial Fluorescence Labeling Plasmids from the Barbier lab). Bright...

...Antibody Guide, which include information about immunofluorescence, conjugates, isotypes, and more. How You ...Basics of Western Blotting Introduction to Immunofluorescence Addgene’s Antibody Guide The project is ...

...will not produce the fluorescent protein while areas that are exposed will fluoresce. With these images,...

... a feature inaccurately. The best example is fluorescent proteins, which often come in closely related...misidentification, we augment the database with new fluorescent protein variants. The future of DNA feature ...