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We narrowed to 637 results for: ESP

Showing: 301 - 320 of 637 results

  1. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...during the production process? These questions are especially important when producing multiple vectors in ... with the most total reads are most likely to correspond to the contaminant. Each contig is then BLASTed...

  2. Of Myc and Men

    Type
    Blog Post
    ...interest to better study them in vivo and in vitro, especially in the mid-late 20th century with the advent ...tumorigenesis.  Using two different synthetic peptides corresponding to linear sequences within the middle of the...

  3. PhD Applications After COVID

    Type
    Blog Post
    ...so reach out to three in case one isn’t able to respond to your email. During my PhD application cycle,...interview from a travel day to an online call in response to challenges on their end. Though the quick changes...

  4. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    .... In this scenario, gRNAs that are “enriched” correspond to genes that confer drug resistance when mutated...library plus Cas9 to generate the mutant cells, respectively (C). Mutant cells are screened (D) and "hits...

  6. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...Infographics Low Combines both words and images; especially useful for conveying and contextualizing data...except for space) Real-time audience engagement and response. High connection levels and interaction. Can bring...

  8. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ... figure organization with a different person responsible for each figure. Others still are more casual...focus and critical mass of participants. This is especially true of journal clubs where the very point is...

  9. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...Mikhaylova, et al. 2015).  Despite these limitations, ExM is a valuable tool, especially when paired with complementary...

  10. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...students. But he also found that his teaching responsibilities left him with limited time for one-on-one ...professional risk involved in setting up a CURE, especially if it’s a new approach within their department...

  11. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...you can inhibit cell cycle regulatory proteins responsible for the transition out of S phase, so cells stay...Methods favoring homology-directed repair choice in response to CRISPR/Cas9 Induced-double strand breaks. Int...

  12. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...AsCpf1 and LbCpf1 (1307 and 1228 amino acids long respectively), both cleave in a staggered pattern similar...rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins...

  13. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...experimental target, SpCas9 and its enhanced variants, eSpCas9 and HypaCas9, are great choices. Check out our ... using a Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9 for site-specific...

  14. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...envelope protein on the virus’s surface and a corresponding receptor in the host cell. Some of these envelope-receptor...infecting neurons and cause minimal inflammatory response, but are not taken up by axon terminals and can...

  15. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...neighboring ssRNA and ssDNA by Cas13 and Cas12a, respectively, to cleave and activate a reporter. The detectable...amplification) or reverse transcriptase (RT)-RPA, respectively. RPA is coupled with T7 transcription to convert...

  16. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...also very important in a non-research context, especially when considering small to mid-sized companies...When you reach out as a student, people tend to respond in a supportive way and are willing to help because...

  19. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...supports reproducibility RSpace was designed in response to requirements that emerged from intensive engagement...developed with these principles in mind, and in response to further input provided by the research communities...

  20. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Gern, J., & Seroogy, C. (2017). Neonatal immune response to rhinovirus A16 has diminished dendritic cell...Greiner, D., & Anderson, D. (2015). Neutrophil Responses to Sterile Implant Materials. PloS One, 10, e0137550...
Showing: 301 - 320 of 637 results