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Showing: 301 - 320 of 628 results

  1. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...complementary and non-complementary to the gRNA, respectively. When complexed with a truncated gRNA, Cas9 ...transcriptional activation or repression at the respective sites.   Many thanks to our guest bloggers Alissa...

  2. PhD Applications After COVID

    Type
    Blog Post
    ...so reach out to three in case one isn’t able to respond to your email. During my PhD application cycle,...interview from a travel day to an online call in response to challenges on their end. Though the quick changes...

  3. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    .... In this scenario, gRNAs that are “enriched” correspond to genes that confer drug resistance when mutated...library plus Cas9 to generate the mutant cells, respectively (C). Mutant cells are screened (D) and "hits...

  5. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ... figure organization with a different person responsible for each figure. Others still are more casual...focus and critical mass of participants. This is especially true of journal clubs where the very point is...

  6. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...Infographics Low Combines both words and images; especially useful for conveying and contextualizing data...except for space) Real-time audience engagement and response. High connection levels and interaction. Can bring...

  7. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...Mikhaylova, et al. 2015).  Despite these limitations, ExM is a valuable tool, especially when paired with complementary...

  8. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...students. But he also found that his teaching responsibilities left him with limited time for one-on-one ...professional risk involved in setting up a CURE, especially if it’s a new approach within their department...

  9. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...you can inhibit cell cycle regulatory proteins responsible for the transition out of S phase, so cells stay...Methods favoring homology-directed repair choice in response to CRISPR/Cas9 Induced-double strand breaks. Int...

  11. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...AsCpf1 and LbCpf1 (1307 and 1228 amino acids long respectively), both cleave in a staggered pattern similar...rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins...

  14. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...supports reproducibility RSpace was designed in response to requirements that emerged from intensive engagement...developed with these principles in mind, and in response to further input provided by the research communities...

  15. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...neighboring ssRNA and ssDNA by Cas13 and Cas12a, respectively, to cleave and activate a reporter. The detectable...amplification) or reverse transcriptase (RT)-RPA, respectively. RPA is coupled with T7 transcription to convert...

  16. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...envelope protein on the virus’s surface and a corresponding receptor in the host cell. Some of these envelope-receptor...infecting neurons and cause minimal inflammatory response, but are not taken up by axon terminals and can...

  17. Viral Production at Addgene

    Type
    Blog Post
    ...reported or posted on the material page for the corresponding catalog item (see maps section for images). ...targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease...

  18. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...Converts A to Y (T or C), X (any base), or C, respectively. gGBE, gTBE, gCBE Glycosylase-based G, T, ..., or C base editors Excises G, T, or C, respectively, to be replaced with another base. References ...

  19. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...shipping. However, there were issues that persisted despite our best efforts. With a little over half of our...time when a picture is worth a thousand words, especially when it comes to protocols. In 2013, we launched...

  20. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...counterparts. Those familiar with viral vectors, and especially those familiar with adeno-associated viruses ...chemogenetic tools: protein tools that are activated, respectively, by substrates, light, or engineered ligands...
Showing: 301 - 320 of 628 results