...world, is not inert. In all seriousness, EGFP/GFP has a real nontrivial propensity to noncovalently dimerize...artifacts in your experiments. Considerable effort has gone into making FPs monomeric, yet many investigators...dimerizing protein of interest fused to a dimerizing FP has potential to form large stable polymers of dimers... monomerized with an A206K mutation [2]. Addgene has many of these constructs and they are designated ...

...joined Addgene's Advisory Board.  Addgene: Your lab has worked extensively with enChIP systems. Can you describe...advantages? Fujii: In the last several years, my lab has been working on development of technologies for biochemical... systems to Addgene in the near future. Addgene: Has your lab been working on any other technologies that...plasmids. The merit of depositing plasmids to Addgene has been spectacular. We've got more than one hundred...

... to monitor cellular perturbations to how CRISPR has made it much easier to generate mouse models.   ...data from the Kim and Joung Labs showing that Cpf1 has lower off-target editing and similar on-target editing...

...proteins (GFP and YFP) and protein tags (c-MYC and HA). We have also created the Monoclonal Antibody Research...community. Thanks to Our Guest Blogger!   David Soll has been the Director of the Developmental Studies Hybridoma...

...science and our plasmid collection (what science has been hot this year and how do you creatively turn...procrastinating choosing their costume?  As our organization has grown, we continue to have an amazing turnout for...

... Broad Institute, John Doench. CRISPR technology has made it easier than ever both to engineer specific...databases annotate the TSS in different ways, and it has been shown that the FANTOM database, which relies...mismatches that can nevertheless still lead to activity has been established, and can be used to identify sites...Perturbation Platform at the Broad Institute and has worked with many Addgenies to help improve the understanding...e141–e141 . https://doi.org/10.1093/nar/gkw583 Rees HA, Liu DR (2018) Base editing: precision chemistry ...

...Adeno-associated virus (AAV) has many features which make it a great viral vector, but its packaging ... Overlapping  Overview: The overlapping strategy has a region of homology, typically ~400-1400 bp long...Drawbacks:  Generally an overlapping split vector has equal or higher transgene expression when compared...split AAV method While the hybrid dual vector system has a slight advantage over the overlapping and trans-splicing...efficiency of transgene expression from split vectors has been inconsistent. A greater understanding of basic...

...Your new antibody has arrived, hooray! Before you jump straight into the exciting new experiments that...good idea to pause and make sure that your antibody has been appropriately validated so that you can have...application. The goal is to show that your antibody has been validated using at least one of these strategies...is binding to something unexpected. This approach has high specificity, but you need to be aware of any...found a cell line in which the gene for your protein has been mutated using CRISPR/Cas9. You check the mutation...

...10 min and then recheck the pH to ensure that it has not drifted. Filter the solution through a 0.22 µm...

... S. University of Baroda, India  Devarshi Gajjar has 10 years experience in the field of bacterial and...biomedicine and astrobiology. During the last 11 years he has studied various aspects extreme life on earth including...educational resources for scientists globally, Addgene has partnered with various community organizations and...

...in previous animal studies suggested that fasting has a positive effect on cancer treatment (Ref 1). As... mutations and clinical responses. This platform has the potential to become an open-source pipeline and...of the plasmids on her poster is pTomo-Ras which has been deposited at Addgene was used to produce the...

...useful research materials and information. Addgene has been successful in helping thousands of sharing scientists...depositors and partners. The Addgene Scientist Team has been developing expertise in answering questions ...from plasmids in the repository. The Outreach Team has been making sure scientists know about the new initiative...

... capsid? A: It is our understanding that AAV-BR1 has the same genome size restrictions as other AAV. Therefore...but reproducible success remains elusive.  There has also been a lot of effort in immunomodulation or ...AAV re-administration but I do not think the field has settled on ‘best practices’ for model organisms.  ...

...Addgene has recently announced the launch of an open-access recombinant antibody resource called NABOR...Detailed figure legend here.     The Trimmer Lab has continued to work to make the sequences of monoclonal...collection and make them publicly available. The lab has also expanded their recombinant affinity reagent ...

...partnership was fruitful and a first version of the study has just been uploaded to the preprint repository BioRxiv... platform; while they are a minority, their help has been essential to push certain projects. The idea...year on, our biggest lesson running the platform has been the realization that there is a great untapped...

...makes a good mentoring program. If your organization has a mentoring program, consider tapping into it and... have going on locally. Your university probably has an alumni directory. Alumni who enter their information... own peer mentoring circle. This type of program has many advantages and I am seeing a lot of success ...

...I was excited to see that Addgene has recently added a new feature for depositing labs to its website...anytime. The CSV is a rich source of information and has data on the date, material, requesting PI & organization...

...Request, an expansion of our viral vectors service, has officially launched! As of today, you can request... on.  Expanding our viral vector service Addgene has offered in-stock viral vectors since 2016, with the...

...continue their careers in the United States. There has been a growing shift away from employers sponsoring...that the researcher is working under in the lab or has won, awards, media reports, and membership in groups...that you are a classically trained ballerina who has performed at Lincoln Center. Your case should only...only support tenure track professors. Casseus Law has seen that research institutions are willing to sponsor... your current research goal is and why your work has impacted your field (i.e. finding vaccines for deadly...The platinum standard for an expert is someone who has cited your work. Immediate steps that you can take...

...changes, and the addition of protein tags. It also has the flexibility to modify the E. coli chromosome,...recombineering system The lambda red recombineering system has three components (Figure 1): 1) Exo, 2) Beta, and... in the center of the sequence. Since lambda red has a higher recombination frequency when the lagging...activating MMR: 1) use a strain of bacteria that has key MMR proteins knocked out or 2) specially design...recombinant clones If a antibiotic resistance gene has been inserted, recombinants can first be selected...