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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 856 results for: HA

Showing: 301 - 320 of 856 results

  1. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...derived from the same cell type; however, their usage has been limited by technical problems. For instance,...binary systems in which transient protein expression has a long-lasting effect, such as Cre- or Cas9-mediated...effects. Third, protein purification (once a protocol has been established) may be less laborious than viral...

  2. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...sequence if necessary. The example plasmid on the right has a total size of 7.3kb, including a 1.2 kb insert....resulting gel image includes a 1kb ladder (lane 1) that has bands ranging from about 500bp to 10kb, with the ...reference point so you can easily assess how far the gel has run. Bonus: The dyes also run at predicted sizes ...

  3. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...Griesbeck of the Max Planck Institute for Neurobiology has been working on genetically encoded indicators of...imaging neurons, Griesbeck adds that this color change has a particular advantage for observing events taking...Maarten Merkx of Technische Universiteit Eindhoven has similar interest in FRET-based sensor proteins, particularly...

  4. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...booklet. What has been the most surprising thing about becoming a PI? Tom Ellis has a one word answer...prepared me for what a PI without a personal assistant has to deal with. My inbox currently stands at about ...

  5. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...studying the genetic underpinnings of these processes has been complicated by the lack of precise genetic tools...Simplified in vivo delivery using SPH mice CRISPRa has largely been limited to in vitro settings due to ...can be delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV. Such an approach... or coding regions. Typically, each gene isoform has a distinct transcriptional start site. Because CRISPRa...

  6. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... is easy to use, functional in most species, and has many applications. That said, SpCas9 is not the only...SpCas9. Cas13a (previously referred to as C2c2), has several unique properties that further expand the...single A, U, or C base pair. In bacteria, once Cas13a has recognized and cleaved its target RNA sequence as...This system, dubbed SHERLOCK (depicted in figure 1) has been used to differentiate strains of Zika virus,...

  7. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ... Cas11-like protein, and a guide RNA. The enzyme has dual activities: processing pre-CRISPR RNA into mature...kilobase-sized gap in the gene editing field. The AbuGoot lab has now developed PASTE, which can “drag and drop” kilobases...PASTE is more specific and efficient than HITI and has higher purity of insertion than both HITI and HDR...therapeutic applications. The potential of CRISPR has yet to fully taken advantage of, but exciting new...

  8. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...that have been in the repository for many years, has now grown into a collection of over 2,400 plasmids... based on 100 structure-guided spike designs and has improved protein yields and stability. This stabilized...virus spreads around the world, the spike protein has had many opportunities to evolve. Several variants... to accelerate research through sharing reagents has never been so important.     References and ...

  9. Hot Plasmids - November 2023

    Type
    Blog Post
    ...off-target effects of gene therapy, Feng Zhang’s lab has developed a system called DIRECTED (Delivery to Intended...on-target delivery with various delivery modalities and has the potential to reduce negative side effects. This... assays like IHC. In fact, Anti-PSD-95 [K28/43R] has been one of the hottest antibodies in Addgene’s collection... collection since we began distribution and has been verified to perform well in standard IHC and western...

  10. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...plasmid transformation, though, a pooled library has hundreds to millions of distinct plasmids, all of...they were prior to amplification. And as anyone who has worked with individual plasmids knows, some plasmids...size and backbone.  Verification Once your library has been amplified, you'll need to verify your library...transduction conditions, you’re ready to transduce! Addgene has a transduction protocol you can follow and a troubleshooting...

  11. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...means the bacterium is not naturally resistant but has become resistant somehow overtime. One fantastic ...on a plate! Antibiotic resistance can arise, and has arisen, for many different reasons. Exposure to antibacterial...presence of a known concentration of antibiotic that has been impregnated into a paper disk, and observing...resistance; it’s a natural process of life and one which has allowed bacteria to evolve and survive for millennia...

  12. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    ...world, is not inert. In all seriousness, EGFP/GFP has a real nontrivial propensity to noncovalently dimerize...artifacts in your experiments. Considerable effort has gone into making FPs monomeric, yet many investigators...dimerizing protein of interest fused to a dimerizing FP has potential to form large stable polymers of dimers... monomerized with an A206K mutation [2]. Addgene has many of these constructs and they are designated ...

  13. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...joined Addgene's Advisory Board.  Addgene: Your lab has worked extensively with enChIP systems. Can you describe...advantages? Fujii: In the last several years, my lab has been working on development of technologies for biochemical... systems to Addgene in the near future. Addgene: Has your lab been working on any other technologies that...plasmids. The merit of depositing plasmids to Addgene has been spectacular. We've got more than one hundred...

  14. Editor's Choice, July 2016

    Type
    Blog Post
    ... to monitor cellular perturbations to how CRISPR has made it much easier to generate mouse models.   ...data from the Kim and Joung Labs showing that Cpf1 has lower off-target editing and similar on-target editing...

  16. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...science and our plasmid collection (what science has been hot this year and how do you creatively turn...procrastinating choosing their costume?  As our organization has grown, we continue to have an amazing turnout for...

  17. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ... Broad Institute, John Doench. CRISPR technology has made it easier than ever both to engineer specific...databases annotate the TSS in different ways, and it has been shown that the FANTOM database, which relies...mismatches that can nevertheless still lead to activity has been established, and can be used to identify sites...Perturbation Platform at the Broad Institute and has worked with many Addgenies to help improve the understanding...e141–e141 . https://doi.org/10.1093/nar/gkw583 Rees HA, Liu DR (2018) Base editing: precision chemistry ...

  18. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...Adeno-associated virus (AAV) has many features which make it a great viral vector, but its packaging ... Overlapping  Overview: The overlapping strategy has a region of homology, typically ~400-1400 bp long...Drawbacks:  Generally an overlapping split vector has equal or higher transgene expression when compared...split AAV method While the hybrid dual vector system has a slight advantage over the overlapping and trans-splicing...efficiency of transgene expression from split vectors has been inconsistent. A greater understanding of basic...

  19. Antibodies 101: Validation

    Type
    Blog Post
    ...Your new antibody has arrived, hooray! Before you jump straight into the exciting new experiments that...good idea to pause and make sure that your antibody has been appropriately validated so that you can have...application. The goal is to show that your antibody has been validated using at least one of these strategies...is binding to something unexpected. This approach has high specificity, but you need to be aware of any...found a cell line in which the gene for your protein has been mutated using CRISPR/Cas9. You check the mutation...

  20. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ... S. University of Baroda, India  Devarshi Gajjar has 10 years experience in the field of bacterial and...biomedicine and astrobiology. During the last 11 years he has studied various aspects extreme life on earth including...educational resources for scientists globally, Addgene has partnered with various community organizations and...
Showing: 301 - 320 of 856 results