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Showing: 301 - 320 of 771 results

  1. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...the fly. But just like the growing PubMed article list, the Genome Engineering Using CRISPR/Cas Systems...questions answered about CRISPRs? Check out the full list of 16 FAQs answered by Le Cong Read Addgene's CRISPR... Addgene Find protocols and gRNA design tools: List of CRISPR protocols developed by a variety of labs...

  2. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...lab, you'll likely find many of the antibiotics listed in the table below. Note, in this post we'll focus...filter unless otherwise specified. The above table lists some antibiotics commonly found in the lab, their...and E. coli strain are compatible! Check out this list of common E. coli genotypes and their natural resistances...

  3. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...could do something like puzzling or crafts, while listening to podcasts made for kids. Plan out meals and ... Micropublication will accept negative data or publish brief, novel findings. Most Micropublications contain...helpful to you no matter what you’re trying to accomplish during this period. ...

  4. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...made to moderate and cross-check all data with published literature as soon as possible after submission... queries.  Looking for a bright, monomeric FP published in the last few years with an emission peak around...Fluorescent Protein Properties provides a master list of all proteins in the database, and the Interactive...

  5. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ... using in an experiment, then spend the evening listen to some bloke at the pub telling me that actually... BSc in Anatomy, the University of Otago had established the Centre for Science communication and I was...discussions about the difference between airborne and aerosolised droplet transmission and how different fabrics...

  6. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...sequenced There are several human disease models established in yeast, including a widely used Parkinson’s...the name of beer and bread. The following table lists some of the biological commonalities and differences...eukaryotic cells and peroxisomes are where methanol metabolism occurs in methylotrophic organisms. K. phaffii...

  7. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...transform your work into a paper or a grant. Here’s a list of tools and resources that might help make writing...library or don’t have time to read the book, this list summarizes the key points of the book. The best ...each week, and there’s advice on how to actually establish that schedule. There’s also tips for setting writing...

  8. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...being experimented on, too.) However, as research published on December 13 shows us, CRISPR/Cas9 techniques...Council. While the Nuffield Council has recently published a report on the social and ethical issues of genome...their research into both novel and previously established ethical issues raised around mitochondrial replacement...

  9. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...why the results you've measured should change established theory. So, what can you do to ensure that your...scientists need to communicate with non-scientists, to establish broader impacts for their work in education and... ask a friend who works in a different field to listen to you practice.  Ask them what they learned from...

  10. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...protein (HRP), primarily used in Western blots and ELISAs, cannot be stored in sodium azide at all, as it...of signaling molecules. Assay Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that...there’s a specific reason you can’t use it for your ELISA and Western, I’d highly recommend sticking with ...

  11. How Addgene Creates Content

    Type
    Blog Post
    ... the example of our Antibody Guide, which was published alongside our new antibody services in the spring...was then assigned to our science communication specialist, Rachel, to write. Identify expertise While ...final review to catch those pesky typos, and hit publish. Making content is an iterative process. We are...

  12. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ....  Review the conference schedule and attendance list. Plan the sessions you will attend. Research the...meal is a great way to build relationships. Make a list of experimental problems or questions you are pondering...Congratulate your connections when they get a new paper published. Stay in touch enough to plan to meet up at the...

  13. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...’re on a profile page. You might be looking at a list of suggested connections and you’ll see the boxes...It’s also a great way to keep track of past accomplishments. Follow all the rules of good resume writing...writing. Include examples of concrete accomplishments in both the technical and “plus skills” areas. Include...

  14. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...Note: After this blog was published, a bioRxiv preprint that questions the conclusion of inter-homologue...with a storm of media coverage. But the paper published August 2nd in Nature gives us even more to talk...Since the embryos produced were not being used to establish a pregnancy, the researchers were able to examine...

  15. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...CytERM with mTurquoise2 and dTomato, which are established monomeric and dimerizing FPs respectively, are...compared with mEGFP or mTurquoise2, which are established true monomeric fluorescent proteins. Concluding... experience that is gathered can be shared by publishing it as (part of) figure 1, uploading it as a preprint...

  16. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...producing the fluorescent protein of interest under realistic conditions, i.e. with low excitation power. By...power experiments. Measuring photostability at realistic powers will provide better insight into the photostability...photostability in living cells provides a much more realistic view of photostability. Selecting a photostable...

  17. Antibodies 101: Conjugation

    Type
    Blog Post
    ...lends itself well to assays like western blots and ELISAs. Chromogenic reactions produce a color change, ...immunohistochemistry, though they are also a common choice for ELISAs.  Biotin Biotin is used in a range of antibody-...BioRender.com.     Many biologists can lead perfectly blissful, productive lives without thinking about these...

  18. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...Albert Einstein College of Medicine has recently published techniques to follow translation in living single...presented a novel technology, back then still unpublished, that allowed the visualization of specific de...combination of FUNCAT and PLA PLA was originally published by the Söderberg lab in Sweden as a method for...

  19. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types... using GFP as a scaffold suddenly seemed very realistic. Envisioning GFP as a substrate promoting dimerization...optogenetics experiments in specific cell populations. Listen to Our Podcast Interview with Connie Cepko Combining...

  20. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ...contributed by Johnna Roose. This post was originally published on Johnna's New Under The Sun Blog and is part...you will likely have is sifting through the long list of titles for something that is actually useful ...Manuscripts by Johnna Roose Dos & Don'ts When Publishing a Scientific Manuscript by Maaike Pols, PhD, ...
Showing: 301 - 320 of 771 results