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TypeBlog Post...evolution and the “x” is the placeholder for the number that identifies the mutation in the evolved TadA...
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Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...promoters. Most existing promoters express in a broad number of cell types, such as inhibitory neurons, rods... -
Lab Automation at Addgene
TypeBlog Post...Handler and we program the handler to fill a specific number of vials with a specific amount of agar. There ... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...to deposit them back with Addgene and expand the number of genes that other classrooms and researchers ... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post...Powerpoint based templates with different column numbers for a good starting point. Adobe also offers free... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog Post...amplification primers. This helped make sure that we had a number of successful sensors to draw on as our assays ... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post...titer your virus. Getting an accurate count of the number of infectious particles in a given viral stock ... -
Plasmids 101: FLEx Vectors
TypeBlog Post...will excise the DNA fragment. By manipulating the number, orientation, and type of target sites that flox... -
The Materials Science of Optogenetics Experiments
TypeBlog Post...during the course of an experiment. There are a number of reasons for this but the primary one is that... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post... good ways to monitor and track them. We have a number of microscopes, including an automated microscope... -
Degrading DNA with Cascade-Cas3
TypeBlog Post...versatility of CRISPR allows you to play with DNA in a number of ways, from small edits that change single base... -
Pooled Library Amplifications
TypeBlog Post...Libraries at Addgene. Library Type Name Number of Plasmids Variable Region Size DNA per Amplification... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog Post...the better choice. Of note, there is no minimum number of markers that needs to be used with spectral ... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...the addition of auxin to the culture medium. Any number of regulatory sequences, including protein enhancers... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post...advantage of this technique is the potentially large number of single clones obtained (on occasion we have ... -
Viral Vectors 101: Voltage Indicators
TypeBlog Post...specific example is attractive due to the large number of sheer molecules and signal you can generate ... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog Post...Nashville, Tennessee has already demonstrated that a number of novel small molecules can bind directly, albeit... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post...things are not quite so simple. Concentration is the number of molecules within a volume. In many biological... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post...about step 3, however, you may be surprised at the number of options available - how do you choose between... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog Post...risk a potential way to dramatically reduce that number for the sake of laboratory convenience. Disclaimer...