Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 532 results for: PAN

Showing: 301 - 320 of 532 results

  1. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...resources (Athey et al., 2017) and many DNA synthesis companies, such as IDT, Genewiz and GenScript, offer tools...Molecular Biology Reference Read about genetic code expansion Browse our plasmid collection page  ...

  2. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...C2c2), has several unique properties that further expand the CRISPR toolbox. We'll cover how Cas13a was ...diversity to the CRISPR toolbox and will continue to expand the applications of CRISPR. Since this post was...

  3. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...through the development of tools that exploit and expand our current knowledge. The Abudayyeh-Gootenberg... Lately, they’ve been working on two tools that expand and exploit CRISPR, both of which we're featuring...

  4. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...then read on for your best options. There are an expanding number of Cas9s with looser PAM requirements compared...Cas9s can be found in “The PAM Requirement and Expanding CRISPR Beyond SpCas9.” Single base pair edits ...

  5. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...video to complement traditional print journals and expand our ability as researchers to reach new audiences...Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in Research Publications Resources...

  6. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...alternatives to GFP is expected to grow with the ever-expanding genetic toolbox generated by the continuous effort...PubMed PMID: 28401765. 15. Takai, Akira, et al. "Expanded palette of Nano-lanterns for real-time multicolor...

  7. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ... used Mango to study RNA-protein complexes (Panchapakesan et al., 2017) and short non-coding RNAs (Autour...27446908. PubMed Central PMCID: PMC4923196. Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein...

  9. Finding Your Science Policy Path

    Type
    Blog Post
    ...than ever thanks to the virtual world that the pandemic has foisted upon us. Action Items:  A simple ...combat sexual harassment in academia or a large company who decides how they are going pursue regulatory...

  10. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...In 1976, the world’s first genetic engineering company, Genentech, was founded.  This commercialization...Hugo De Vries: from the theory of intracellular pangenesis to the rediscovery of Mendel. Comptes Rendus ...

  11. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...Single cells from a stable cell pool were then expanded for an additional 3 weeks under blasticidin selection...stable cell pool (pool) and from individual cell expansions (monoclonal, 1-5). Wild-type A549 cells do not...

  12. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...therapies can be developed and applied. The current pandemic serves as a striking example of the applicability...opportunities The world of science is vast and ever-expanding. While it can be tempting to try to plan out a...

  14. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...gRNA oligos, preferably as an oligo pool. Some companies that synthesize oligos would even accept the Output_Short...through https://wermelinglab.com/.   References 1. Panda, Sudeepta Kumar, et al. "Green listed—a CRISPR screen...

  15. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...CRISPR CRISPR-Cas can harness the power of MoClo to expand both targetability and effectiveness. Perhaps the...tools, it should be easy enough for researchers to expand the Fragmid toolkit to facilitate other lentivirus...

  16. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...detect proteins is referred to as staining. This panel of antibodies is used to both identify the cell ...flexibility in which proteins you can investigate in each panel. An advantage of flow cytometry is the ability to...

  17. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...preferences, which they defined thoroughly. Plus, they expanded CGBE target scope by creating BE variants with...CGBEs. They found that C → G editing was often accompanied by small deletions in their target loci (between...

  18. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...LaManna, published Mar 8, 2016. The updated and expanded version by Nyla Naim was published Sept 3, 2020...ja512664v  Jain PK, Ramanan V, Schepers AG, Dalvie NS, Panda A, Fleming HE, Bhatia SN (2016) Development of Light-Activated...

  19. Deep Dive: qPCR

    Type
    Blog Post
    ...are cases in which it is an appropriate choice (Panina, 2018). Look for studies specifically aimed at ...real-time RT-PCR. J. Immunol. Methods 283:291–306.Panina, Y., Germond, A., Masui, S. et al. Validation of...

  20. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids by offering ...protein function and signaling networks continues to expand. The labs of Jesse Rinehart and Dieter Söll at ...
Showing: 301 - 320 of 532 results