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We narrowed to 538 results for: PAN

Showing: 301 - 320 of 538 results

  2. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...Distribution tab, you’ll find an Indel plot (left panel) which displays the inferred distribution of indel... contain the indel. The discordance plot (right panel) shows the level of disagreement between the non-edited...

  3. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...resources (Athey et al., 2017) and many DNA synthesis companies, such as IDT, Genewiz and GenScript, offer tools...Molecular Biology Reference Read about genetic code expansion Browse our plasmid collection page  ...

  4. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...then read on for your best options. There are an expanding number of Cas9s with looser PAM requirements compared...Cas9s can be found in “The PAM Requirement and Expanding CRISPR Beyond SpCas9.” Single base pair edits ...

  5. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...through the development of tools that exploit and expand our current knowledge. The Abudayyeh-Gootenberg... Lately, they’ve been working on two tools that expand and exploit CRISPR, both of which we're featuring...

  6. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...C2c2), has several unique properties that further expand the CRISPR toolbox. We'll cover how Cas13a was ...diversity to the CRISPR toolbox and will continue to expand the applications of CRISPR. Since this post was...

  7. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...video to complement traditional print journals and expand our ability as researchers to reach new audiences...Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in Research Publications Resources...

  8. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...alternatives to GFP is expected to grow with the ever-expanding genetic toolbox generated by the continuous effort...PubMed PMID: 28401765. 15. Takai, Akira, et al. "Expanded palette of Nano-lanterns for real-time multicolor...

  10. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ... used Mango to study RNA-protein complexes (Panchapakesan et al., 2017) and short non-coding RNAs (Autour...27446908. PubMed Central PMCID: PMC4923196. Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein...

  12. Finding Your Science Policy Path

    Type
    Blog Post
    ...than ever thanks to the virtual world that the pandemic has foisted upon us. Action Items:  A simple ...combat sexual harassment in academia or a large company who decides how they are going pursue regulatory...

  13. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...Single cells from a stable cell pool were then expanded for an additional 3 weeks under blasticidin selection...stable cell pool (pool) and from individual cell expansions (monoclonal, 1-5). Wild-type A549 cells do not...

  14. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...therapies can be developed and applied. The current pandemic serves as a striking example of the applicability...opportunities The world of science is vast and ever-expanding. While it can be tempting to try to plan out a...

  15. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...In 1976, the world’s first genetic engineering company, Genentech, was founded.  This commercialization...Hugo De Vries: from the theory of intracellular pangenesis to the rediscovery of Mendel. Comptes Rendus ...

  16. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...detect proteins is referred to as staining. This panel of antibodies is used to both identify the cell ...flexibility in which proteins you can investigate in each panel. An advantage of flow cytometry is the ability to...

  17. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...gRNA oligos, preferably as an oligo pool. Some companies that synthesize oligos would even accept the Output_Short...through https://wermelinglab.com/.   References 1. Panda, Sudeepta Kumar, et al. "Green listed—a CRISPR screen...

  18. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...CRISPR CRISPR-Cas can harness the power of MoClo to expand both targetability and effectiveness. Perhaps the...tools, it should be easy enough for researchers to expand the Fragmid toolkit to facilitate other lentivirus...

  20. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...preferences, which they defined thoroughly. Plus, they expanded CGBE target scope by creating BE variants with...CGBEs. They found that C → G editing was often accompanied by small deletions in their target loci (between...
Showing: 301 - 320 of 538 results