We narrowed to 653 results for: PPL
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TypeBlog Post...a thermostable Cas9 be useful? It can enable applications where reagents must be stored at elevated temperatures...stability under harsh conditions. One example application from the publication is the removal of unwanted...
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HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post... the future, this stitching technique could be applied to other epitopes and scaffolds to build a more...antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog Post...executed between a TTO and Addgene, whose terms apply to all future deposits of materials with Addgene...executed between a TTO and Addgene, whose terms apply to all future materials requests from Addgene MTA... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog Post...are many different kinds of grants that you can apply for. If you are in a lab, your PI is likely working...working on a number of them. Find one to apply to or offer to help with lab grant writing. You may not get... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog Post...Unfortunately, the vast majority of antibodies are supplied with no sequence information, leading to problems... specific activity for each reagent batch. To supplement this, Addgene will be creating a crowdsourced... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...mediate homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication at Addgene...site specified by the gDNA. Depending on your application, this could be favorable or unfavorable. In a... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog Post...parasites, to symbionts, to genes and the labels applied to them were vague or contradictory, owing in part...seen as an ubiquitous tool that can be diversely applied to many different experiments. Addgene was founded... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog Post...permission from the Genetics Society of America. Applications of this system Dickinson et al. have developed... in cell culture. In addition to CRISPR/Cas9 applications, SEC selection could replace other, more restrictive... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post...unwanted immunogenicity. The 3-D models can be applied to corroborate the occurrence and extent of the... together can inform decisions about in vivo application and clinical testing. This would pave the way... -
K. phaffii: Rising to the Occasion
TypeBlog Post...2017). Beyond human relevance, yeast have many applications in food science and biotech due to its fermentation... ancient yeast strains and metazoan cells. Applications for K. phaffii There’s a number of different... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...variants. Image adapted from Aggarwal et al…, Supplementary Figure 3. Figure 3: pH titration...variants. Image adapted from Aggarwal et al…, Supplementary Figure 2. Improved trafficking and localization... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog Post... have profound impacts on the development of applications with the potential to save lives. While most...daunting because they have no clear cut answers, I applaud the stem cell community for addressing these important... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...actuators that could be used in a variety of applications. For example, you could use them to lyse cells...matrices) in response to pH changes. Beyond these applications, purified R bodies could also perform as actuators... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...; only two plasmids are required per system. Applications of these systems The large dynamic range of ...suitable for various scientific or engineering applications. References Refactoring and optimization ... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog Post...wanted to continue doing research, Doench went on to apply to graduate school at MIT. Doench excitedly remembers...Harlow’s group at Harvard Medical School and then applied to work at the Broad Institute: “The model that... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post...month, deciding on the best protein for a given application is a challenge. To help keep you abreast of the...biliverdin as a cofactor and so you may need to supplement your cells with biliverdin for maximal brightness... -
Targeted Mutagenesis with EvolvR
TypeBlog Post...the key components of EvolvR and its potential applications. Challenges of existing mutagenesis methods...more than one mutation near the target site. Applications of EvolvR The Dueber Lab used EvolvR to discover... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post... protein purification with OptoNBs The first application for OptoNBs takes us in vitro: light-controlled...OptoNBs to control cell signaling The second application for OptoNBs takes us into cells: light-based ... -
Clear and Accessible Writing
TypeBlog Post...principles we used in our daily content writing applied to alt text. We also were aware of the need for...start small with alt text or immediately dive into applying these four steps elsewhere, engaging in this practice... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...function relationships or, in more practical applications, allow a protein to be more effectively used...the creation of many new tools and potential applications. But this focus on development, along with the...