We narrowed to 651 results for: PPL
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TypeBlog Post... the future, this stitching technique could be applied to other epitopes and scaffolds to build a more...antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based...
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The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog Post...executed between a TTO and Addgene, whose terms apply to all future deposits of materials with Addgene...executed between a TTO and Addgene, whose terms apply to all future materials requests from Addgene MTA... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog Post...kinetics) that makes them suitable for different applications. We recently released a new variant of jGCaMP7... been developed and are suited for different applications. The iGABASnFR GABA sensor (pAAV.hSyn-FLEX.iGABASnFR... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog Post...are many different kinds of grants that you can apply for. If you are in a lab, your PI is likely working...working on a number of them. Find one to apply to or offer to help with lab grant writing. You may not get... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog Post...makes them more adaptable to a broad range of applications. Recently, the Spiess lab developed a set of...Institute focuses on proteomics: developing and applying new technologies to quantify proteins in tissues... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog Post... Biological Resource Center and the Center for Applied Plant Sciences at The Ohio State University. Established... Biological Resource Center and the Center for Applied Plant Sciences at The Ohio State University. In... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post...the gRNA design process and rules for various applications is necessary going forward. The standardization...Alt-NHEJ), has been harnessed for CRISPR knock-in applications. CRISPR 101: Targeting RNA with Cas13a (C2c2... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog Post...Unfortunately, the vast majority of antibodies are supplied with no sequence information, leading to problems... specific activity for each reagent batch. To supplement this, Addgene will be creating a crowdsourced... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post...a thermostable Cas9 be useful? It can enable applications where reagents must be stored at elevated temperatures...stability under harsh conditions. One example application from the publication is the removal of unwanted... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog Post...Science magazine. That one I just could not resist applying for. Not that I knew anything about being an editor...constellation of interests and skills than what fits for applied research. I focus in general terms on a couple ... -
10 Great Guest Posts We're Thankful For
TypeBlog Post... membranes. Read the full post for potential applications of R-bodies and to find a video of them in action... to your DNA sequence of interest. Potential applications of the system include activation and repression... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...mediate homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication at Addgene...site specified by the gDNA. Depending on your application, this could be favorable or unfavorable. In a... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog Post...parasites, to symbionts, to genes and the labels applied to them were vague or contradictory, owing in part...seen as an ubiquitous tool that can be diversely applied to many different experiments. Addgene was founded... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog Post...permission from the Genetics Society of America. Applications of this system Dickinson et al. have developed... in cell culture. In addition to CRISPR/Cas9 applications, SEC selection could replace other, more restrictive... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post...unwanted immunogenicity. The 3-D models can be applied to corroborate the occurrence and extent of the... together can inform decisions about in vivo application and clinical testing. This would pave the way... -
K. phaffii: Rising to the Occasion
TypeBlog Post...2017). Beyond human relevance, yeast have many applications in food science and biotech due to its fermentation... ancient yeast strains and metazoan cells. Applications for K. phaffii There’s a number of different... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...variants. Image adapted from Aggarwal et al…, Supplementary Figure 3. Figure 3: pH titration...variants. Image adapted from Aggarwal et al…, Supplementary Figure 2. Improved trafficking and localization... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog Post... have profound impacts on the development of applications with the potential to save lives. While most...daunting because they have no clear cut answers, I applaud the stem cell community for addressing these important... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...actuators that could be used in a variety of applications. For example, you could use them to lyse cells...matrices) in response to pH changes. Beyond these applications, purified R bodies could also perform as actuators... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...; only two plasmids are required per system. Applications of these systems The large dynamic range of ...suitable for various scientific or engineering applications. References Refactoring and optimization ...