We narrowed to 399 results for: PREP

1. Antibodies 101: Selecting the Right Antibody

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   ...different samples and experimental conditions. Be prepared to troubleshoot and be sure to include proper ...

2. Antibodies 101: Secondary Antibodies

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   ...architecture of the indirect approach is as follows: Prepare the sample. Incubate the sample with the primary...

3. Transferable Skills Guide: Problem Solving

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   ... of contexts. As such, scientists are uniquely prepared to be strong problem solvers both in and out of...

4. Human Germline Editing Using CRISPR

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   ...Note: After this blog was published, a bioRxiv preprint that questions the conclusion of inter-homologue...

5. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   ...publishing it as (part of) figure 1, uploading it as a preprint, or documenting it in some other way. Besides ...

6. Transferable Skills Guide: Cross-team Communication

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   ...miscommunication and b) find practical solutions.  My PhD prepared me remarkably well for this task. Good scientists...

7. Addgene's Tips for Plasmid Quality Control

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   ... over 2000 primers. Two 96-well PCR plates are prepared with the primer stocks for forward and reverse...

8. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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   ...subscription), archived on PubMed Central, or in preprint onBioRXIV. One of the biggest issues you will ...

9. Engaging with science and society at pgEd

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   ...running these events will be important both in preparing you for future work in these areas, and in helping...

10. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ...riboprobes are created, the WISH protocol involves preparation of the tissue sample, several washes, and temperature...

11. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    .... cerevisiae met deficiencies (manuscript in preparation), indicating that Met protein functions have ...

12. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    ...of our viral vectors blog posts Find tips for preparing AAVs View our Beginner's Guide to Viral Vectors...

13. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    ... to mouse modeling now complete, you should be prepared to plan an experimental strategy that uses genetically...

14. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ...of the line. With these tips in mind, you’ll be prepared to run an ELISA in your lab!   References and...

15. Making CRISPR Plasmids Using Fragmid

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    ...always, we recommend checking your cloning via a miniprep and restriction digest, and then plasmid sequencing...

16. Antibodies 101: Stripping and Reprobing Western Blots

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    ...that section of the blot unusable for reprobing. Preparing your western blot Run your gel and membrane transfer...

17. Science communication: 9 Strategies to Get Your Foot in the Door

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    ...list. 7) Internships – Learn on the job Nothing prepares you better than learning on the job. Try to get...

18. Viral Vectors 101: Calcium Sensors

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    ...to go. Pro tip: Vectors with ready-to-use AAV preparations are labeled with the green virus particle on...

19. Viral Vectors 101: Virus Safety

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    ...If administering virus to mice, part of your preparation should be doing your best to be well-rested and...

20. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    ...first discovered as a contaminant of adenovirus preparations. It is an icosahedral, non-enveloped virus that...