We narrowed to 582 results for: RET
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TypeBlog Post...trainee (even if you’re an oldbe trainee!) can be pretty nerve-wracking. So how can you be the best trainee...gives twofold benefits: you may learn faster and retain more information plus your mentor will get you ...
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Getting the Most from Your Lentiviral Transduction
TypeBlog Post..., A., Garnier, A. “Downstream processing of oncoretroviral and lentiviral gene therapy vectors.” Biotechnology...conditions on the production of lentiviral and retroviral vectors.” Human Gene Therapy Clinical Development... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog Post...salts may be preferable if the protein product is secreted to the medium or if slow expression is required...required due to solubility concerns. Insoluble and Secreted Proteins: The most common purification protocols... -
Arf GTPase Family
TypeCollection...Mammalian (pEGFP-C1) GEF Fbxo8 26269 319 Gateway Return to top The content for this page was generated ... -
Transferable Skills Guide: Time Management
TypeBlog Post...entire experiment, but you’ll have to spend the entirety of each day doing the experiment. The second way...during my day according to my priorities, I have a pretty good idea about the specific things I’ll be doing... -
Five Popular Model Organisms
TypeBlog Post... (Zuber et al., 2009). By injecting mice with retroviral vectors that harbored different mutations found...21415126. PubMed Central PMCID: PMC3082451. Pfeiffer, Barret D., et al. "Refinement of tools for targeted gene... -
Plasmids 101: Biotinylation
TypeBlog Post...papers and webpages are listed below: EMSA (Electrophoretic Mobility Shift Assays) ELISA (Enzyme-Linked...Schwartz, S. A. “Biotinylated probes in the electrophoretic mobility shift assay to examine specific dsDNA... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post...composition. So back to the drawing board. Cycle 3: A Return to HQ Figure 3: Even Blugene is practicing...acquire gear that’s only good once. Even when things return to relative normalcy, I’ll be incorporating that... -
Tips for Getting a Faculty Position
TypeBlog Post...include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...This means all newly synthesized sequences are retained by the original damaged DNA molecule. Unlike in...invasive strand is displaced from the template and returned to the processed end of the non-invading strand... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog Post..., you’ll first need to learn a little bit of theoretical background on the different parameters that your... tumor sample You made it! So much about the theoretical background. Let’s dive into the praxis of gating... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post...collection includes 26 unique antibodies that bind discrete subunits or particular subunit pairs. Some of ...researchers realized they needed specific tools to interpret the roles of individual integrins. The Ha lab ... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog Post...pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde) pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde) pGP-AAV-syn-FLEX-jGCaMP8m-WPRE... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...that affect the frequency of multimerization. Theoretically, as long as all the plasmid features remain ... self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. ... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post... is required in this process because result interpretation might be difficult. The low density of mycoplasmas...by these bacteria can hinder correct result interpretation. Also, the signals from the nuclear area make... -
Antibodies 101: Validation
TypeBlog Post...you see that there is high RNA expression in the retina and in parts of the brain, but almost none in the..., you will see a strong antibody signal in the retinal sample, but little to no signal in the liver sample... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post... try research? Personally, I have always been a pretty curious person. I like to ask questions and think...that you don’t think you’re qualified for. Wayne Gretzky’s “You miss 100% of the shots you don't take” quote... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...entire plate was scanned for V5 tagging using an Operetta imaging system. Dewari et al, eLife 2018;7:e35069...follow when planning epitope tagging experiments: Retrieve Ensembl Transcript ID(s) of your favourite gene... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post... technology has been used to optimize protein secretion and expression in the biotech workhorse yeast ...Modular Toolkit for Generating Pichia Pastoris Secretion Libraries.” ACS Synthetic Biology, vol. 6, no.... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post... proper behavioral readout that allows you to interpret the results of your experiment. Like many things...optogenetics has already been proven. Therefore, a concrete, well defined, and robust behavioral output is...