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We narrowed to 741 results for: REV

Showing: 301 - 320 of 741 results

  1. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...tethered to a repressive domain, such as KRAB, to prevent efficient transcription from sgRNA targets. CRISPRi...gain of function screens. These screens can help reveal the function of lowly expressed genes or genes ... sgRNAs were selected and ranked based on the previously established sgRNA rules and  potential on- and...

  2. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...more about how nanobodies are identified in this review. If you just want to use the nanobodies described...picking a secondary nanobody, it’s important to review a few characteristics of your primary antibody:...Nanobodies: natural single-domain antibodies.” Annual review of biochemistry 82 (2013): 775-97. PubMed PMID:...

  3. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...experience. Along with the updated search, we’ve also revamped our homepage and mascot Blugene (check back on...search terms and potential results. Just as in the previous layout, the number of plasmids matching each filter...the source organism for a gene insert. (e.g. S. cerevisiae) Plasmid Type: Choose an empty backbone if you...

  5. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ... for granted. In this post, we take a moment to review what exactly you are measuring and how to interpret...starting from the beginning. Before trying again, revisit your purification protocol. Are any of your materials...and quality than we have covered here! Be sure to review resources on the specific instrument you use and...

  6. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...way to improve the photon budget. Of course, “reverting” to small molecule dyes seems daunting—no one ...enzyme variant tag that reacts specifically and irreversibly with a small substrate ligand motif attached...strategies, several groups—including mine—have been revisiting the old chemistry of dyes. The first synthetic...

  7. CRISPR Antimicrobials

    Type
    Blog Post
    ... both favors antibiotic sensitive bacteria and prevents sensitive bacteria from acquiring resistance genes...settings where antibiotic resistant bacteria have previously flourished.   Figure 2: Two-phage strategies...Infection with the lytic phage kills bacteria not previously infected by the lysogenic phage, rendering the...

  8. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively to screen...heterologous systems (e.g. mammalian proteins in S. cerevisiae). While this may be difficult to resolve for...Kamuzinzi, R., Ledent, V., Blaudez, D., Sanders, D., Revuelta, J.L., Boles, E., André, D., and Frommer, W.B....

  9. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...the limitations associated with SpCas9. Cas13a (previously referred to as C2c2), has several unique properties...adopts an enzymatically “active” state rather than reverting to an inactive state like Cas9 or Cpf1. As a result... Recombinase Polymerase Amplification (RPA) or Reverse-transcription RPA (RT-RPA), respectively. Amplified...

  10. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...itself to be quite the mental puzzle if you lack previous experience with it. Don’t worry, we’ve got you...to be in the same (forward) or in the opposite (reverse) orientation? The answers to questions like these...plasmid map and identify any mismatches that are preventing your overhangs from aligning properly. Do this...

  11. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...antibodies to simian virus 5 and their use in revealing antigenic differences between human, canine and... et al., 2024). In this new variation on their previous sensor STReTCh, the 11-amino acid BiT fragment...excitingly, assembly of the split luciferase is reversible, making PILATeS suitable for live imaging with...

  12. Hot Plasmids - November 2023

    Type
    Blog Post
    ...Incorporating reverse transcriptase (RT) variants, PE6a and PE6b are 500–800 bp smaller than previous editors...reprogram delivery vehicles like lentiviral vectors, CreVLPs, and eVLPs for in vivo and in vitro applications...

  13. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...terrible disease that is, unfortunately, completely preventable. Mainly caused by exposure to asbestos, mesothelioma...This drug (known as an angiogenesis inhibitor) prevents the growth of new blood vessels required to sustain...treatment get, there still remains the problem of prevention. Asbestos exists pretty much everywhere, still...

  14. The Challenges of Cell Culture

    Type
    Blog Post
    ... in a separate backup liquid nitrogen store to prevent loss from equipment malfunctions. One Master vial...approach, the many mistakes of the past can be prevented and we can progress faster to desired clinical...years on: the good, the bad and the ugly."Nature Reviews Cancer 2.4 (2002): 315-319. PubMed PMID: 12001993...

  15. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...efficiently by (1) improving editing accuracy by preventing re-editing and (2) optimizing “cut-to-mutation... was that these “blocking mutations” mutations prevent CRISPR from re-cutting the target sequence once... mutation incorporation probabilities our data revealed, and found that for generating a homozygous HDR...

  16. Five Popular Model Organisms

    Type
    Blog Post
    ...(Pfeiffer et al., 2010). Yeast (Saccharomyces cerevisiae) Yeast, one of the simplest eukaryotic organism...190-195. Liu, Wei, et al. "From Saccharomyces cerevisiae to human: The important gene co‑expression modules...in therapeutic drug discovery." Pharmacological reviews 63.2 (2011): 411-436. PubMed PMID: 21415126. PubMed...

  17. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...the problematically low fluorescent intensity of previous versions of Brainbow. Developed from Brainbow ...visual system, which has been difficult to study previously (Hadjieconomou et al., 2011). Cell tracing in...distances across multiple fields of view, whereas previous Brainbow technologies were useful for discriminating...

  18. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...Site-Specific Integrase Gene Editing (PASSIGE), their previously-reported technique for precisely integrating ...P. Bentley The Weissman and Gilbert Labs have previously deposited their CRISPRoff system — a programmable...the cells you are studying. Antibodies can help reveal cellular identities by targeting marker proteins...

  19. Tips for Getting a Faculty Position

    Type
    Blog Post
    ... details. You DO NOT need to present a detailed review of your field or the finer points of your techniques...avoid the temptation to provide too many details. Reverse engineer your talk. Identify the key points you...This means that once you get an interview, your previous success will not necessarily translate into getting...

  20. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...gathering data. Former lab members can give open, revealing information about the culture and lab head. While...intimidation, sexual harassment Isolation – including preventing access to opportunities, physical or social isolation...record notes and observations so you have data to review when making a decision. If you don’t see a culture...
Showing: 301 - 320 of 741 results