We narrowed to 763 results for: SCR

... The one-pot saturation mutagenesis technique described in Wrenbeck et al is a PCR-based approach for ...library that’s ready to be tested in a functional screen of your liking. This method is similar to site-directed... screen...

...site-specific incorporation of phosphoserine (as described below) provides researchers with a valuable tool...context-specific effects of UAG suppression, as described (6). To determine the stoichiometry of phospho-incorporated...phospho:non-phospho species on PhosTag gels as described above. Reagents and protocols Sep-OTSλ plasmid...

...pathological condensates, including oncogenic transcription, proliferation of leukemia cells, and assembly...easily visible chromoproteins in a cell-free transcription-translation system (Figure 6). This approach...CRISPRkit uses a cell-free system to carry out transcription and translation of chromoprotein and gRNA plasmids...

...applications, including transgenesis, gene-trap screens, and gene editing. How does the piggyBac™ system...effects, such as with signaling molecules or transcription factors, or when the gene is constitutively-..., since there is no selection, you will need to screen somewhere between one hundred and one thousand ...

...promoter/enhancer regions. Blue/white screening can be used for screening of transformants in cloning process...Optimized STARR-seq (Self-Transcribing Active Regulatory Region) : Genome-wide screening of enhancers using ... protein half-lives, making them useful as transcriptional reporters, but are less-than-ideal protein ...mammalian systems. ID Plasmid Luciferase Type(s) Description PI 64784 pGL3-Basic-IRES Firefly Insertion of...Vector for investigating regions controlling transcription Debrya Groskreutz 18760 MSCV IRES Luciferase...Vector for investigating regions controlling transcription Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla...Vector for investigating regions controlling transcription Pete Stecha 106292 pLS-SV40-mP-Rluc Renilla ...

...VP16 transcriptional activation domain). For simplicity, other promoter elements and transcription machinery...Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9-VPR transcriptional activator..., the TetR protein binds to tet O, blocking transcription of the downstream gene. If tet or one of its...to tetracycline and releases tet O, enabling transcription (Figure 1). While TetR and tet O could be a ...tetracycline-controlled transactivator (fusion of TetR with VP16 transcriptional activation domain); rtTA: reverse tetracycline-controlled...to eliminate binding of endogenous mammalian transcription factors to further minimize background expression...plasmids use "TetO" or "Tet-O" in their name or description, meaning they contain a TRE or tet O and can ...

...below. Return to top Cas9 SAM transcriptional activation plasmids and screening library CRISPR/Cas9 Synergistic...RNA 48137 : PX165; SpCas9 Return to top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries...and mouse) for performing genome-wide knockout screens. The libraries are available in two formats: 1 ...) is an engineered protein complex for the transcriptional activation of endogenous genes. It consists...database (23,430 isoforms) for gain-of-function screening. For more information, please visit the Addgene... references are below. Available plasmids are described below: 61591 : PX601; CMV-driven SaCas9; U6-driven...sgRNA to a constitutively Cas9-expressing mouse. Described here are AAV vectors that can be combined with...

...Antibody-independent methods may include analysis of transcriptomic or proteomic data from several different tissues... feasible and (2) large-scale proteomic and transcriptomic datasets already exist.   Example: You want...tissues. You know that there is a wealth of transcriptomic data from this sample type, so you use your...

...brightness of individual fluorophores. Table 1 describes the brightness of a few commonly used fluorophores...brightness levels on your smartphone or computer screen), and you should be able to properly label many...panel! Designing a Panel In this example, I will describe a basic panel (Table 2, Figure 5) for a hypothetical...

... drug discovery by big pharma (i.e. HTRF and LanthaScreen) (Degorce et al., 2009), measuring intracellular...are critical in setting up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET...it all together The FRET donors and acceptors described above can be paired together to measure a wide...

...: dCas9 fused to transcriptional activators or repressors can modulate transcription in a reversible manner...palindromic repeat sequences. (1) The CRISPR array is transcribed to make the pre-CRISPR RNA (pre-crRNA). (2) The...in vitro. In 2013, Cong et al. and Mali et al. described CRISPR-based genome editing in mammalian cell ... of new applications. The first CRISPR papers described two main categories of genome edits. First, the... locus makes it especially amenable to genetic screens. Pooled gRNA libraries can be used to identify ...are available for gene knockout, as well as transcriptional activation or repression. Combined with the...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology...

...Empty Backbones Plasmid Description Industry Return to top Blue Plasmid Description Industry Return to top...top Cyan Plasmid Description Industry Return to top Green Plasmid Description Industry Return to top mEmerald... mEmerald Plasmid Description Industry Return to top Yellow Plasmid Description Industry Return to top...top Orange Plasmid Description Industry Return to top Red Plasmid Description Industry Return to top Photoactivatable...photoactivatable fluorescent proteins. Plasmid Description Industry Return to top Content last reviewed:...

...Library Name Type PI Description SARS-CoV-2 Spike (S) Ectodomain and RBD Libraries Screening Timothy Whitehead...scientists. Antibodies ID Recombinant Antibody Description PI 211756 Anti-SARS-CoV-2 Nucleocapsid Protein...Plasmid Distribution to Industry page. ID Plasmid Description Industry PI Additional Addgene resources SARS-CoV... SARS-CoV, MERS, BAT-CoV Plasmids ID Plasmid Description Industry PI Due to export control regulations...plasmids related to these genes below. ID Plasmid Description Industry PI Additional Addgene resources ACE2... protease (Mpro) activity reporters Reverse transcriptase, protease, and polymerase constructs for SARS-CoV...Neutralizing nanobody plasmids And more! ID Plasmid Description Industry PI Addgene blog Finding nucleic acids...

... ID Plasmid Depositing PI Description Golden Gate TALEN 2.0 38142 RCIscript-GoldyTALEN Daniel Carlson ...new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic...with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI FusX TALEN Assembly System Contains...expression of TALENs from a truncated CAGs promoter. RCIscript-GoldyTALEN is designed for in vitro synthesis ...strong nuclear localisation tags and repress transcription initiation when targeted to DNA sequences within...destination vectors can be used to create TALE-transcription factors and are used at the final assembly step...TALEN kit, (ii) a lacZ fragment for blue/white-screening in E.coli , (iii) CAG promoter and Kozak sequence...

... (V70) (Plasmid #188537) . These reagents are described in detail in Mohler et al. (2023) and help deliver...at the central position. Introducing Hi-P for screening phosphorylation-dependent protein-protein interactions...domain of NEDD4-2 (Pooled Libraries #111705-8) . Screening kinases The Mode #1 phosphosite library can also...Phosphorylated Isomers (iSPI) The iSPI resource described in Galloway et al. (2022) is adapted from the ...Iterative Synthetically Phosphorylated Isomers (iSPI) described in: Gassaway, B. M., Li, J., Rad, R., Mintseris...in a new window) rEcoli XpS and a new pSerOTS described in: Mohler, K., Moen, J. M., Rogulina, S., & Rinehart..., 551 KB) Human Serine Phosphopeptide Library described in: Barber, K. W., Muir, P., Szeligowski, R. V...

...- a CRISPR Screen Tool (Link opens in a new window) Design gRNAs for custom CRISPR screens targeting a...in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's downloadable eBook about...CRISPR pooled libraries for large-scale functional screens. Available for knockout, activation, inhibition...For use with genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology. Developed by the Xiaole ...batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new window) ...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...started by the Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene...

...at the bottom of this page. For more in depth descriptions of each serotype, check out Addgene's blog on...Browse Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306 pAAV-FLEX-tdTomato... Browse Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-FLEX-tdTomato CAG tdTomato...Browse Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP CAG EYFP...Browse Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...Browse Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...

... by abundant transcripts, leading to falsely lowered levels for less abundant transcripts. However, adding...can even be used to detect a single copy of a transcript. But this sensitivity can sometimes cause as ...

...Other tyrosine recombinases include Dre, R, Nigri, SCre, VCre, and Vika. These systems work by sequential...light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...Return to top Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...Return to top Dre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...Site-Specific Recombinase Plasmids ID Plasmid Description Gene/Insert Vector Type Promoter PI Return to...target site (lox, FRT, rox, etc.) ID Plasmid Description Vector Type PI Additional Addgene resources Use...clones, and making transgenic mice. ID Plasmid Description Gene/Insert Vector Type PI Pooled Libraries Pooled...

...find more information. Delivery Method Species Description Article PI Lentivirus Human Expression of human...human Klf4, Oct4, c-Myc, and Sox2 as VP-16 transcriptional activating fusions in single lentiviral vectors...human iPSC generation OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts...Progenitor Cells Lentiviral Human Cooperative Transcription Factor Induction Mediates Hemogenic Reprogramming...Oligodendrocyte Progenitor Cells Lentiviral Mouse Transcription factor-mediated reprogramming of fibroblasts...fibroblasts into functional astrocytes by defined transcription factors. Stem Cell Reports. 2015 Jan 13;4(1)...Neurons Lentiviral Human Chemical modulation of transcriptionally enriched signaling pathways to optimize the...