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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 536 results for: SHI

Showing: 301 - 320 of 536 results

  1. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ... they looked for a biological explanation, a relationship between the genetic dependencies they identified... ancestries. While there were some exciting relationships identified, 48% were found to be artificial,...

  3. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...budget narratives, logic models, established partnerships and collaborations, and commitments to and proof...close-out a grant. Grant professionals also build relationships with foundations and government grantmakers....

  5. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ... If you are miserable at home or work, your relationships will suffer in both the short run and the long...agree on before you get enmeshed in a serious relationship. #5 Thou shalt not sweat the small stuff The...

  6. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...vectors expressing BDNF in the hippocampus causes a shift towards antidepressant behavior in rats in the forced...neuroscience to reveal insights into the complex relationship between genes, neurons, and behavior. There ...

  7. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...mouse an attractive tool to probe gene-function relationships in defined substructures of the CNS as well ...Rochester Medical Center. He studies gene-function relationships in the CNS using CRISPRa as a member of the ...

  8. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...represents the proportion of cells that have either a frameshift or 21+ bp indel. This score is a useful measure...Represents the proportion of cells that have either a frameshift or 21+ bp indel. This score is a useful measure...

  10. Bioinformatics at Addgene

    Type
    Blog Post
    ...and how our search for solutions led us to a partnership with Seqera Labs.              DNA sequencing...Creating a bioinformatics workflow in Nextflow Establishing a complete pipeline however, required more than...

  11. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...be time-consuming or even very frequent, but establishing a few minutes every month dedicated to thinking...Originals how non-conformists move the world. Penguin Publishing Group. Day, F. (2019). Embrace your weird face...

  12. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...Antibody here!   Anderson, Z., Zhu, H., Riedel, T., Moshinsky, D., Meijers, R. (2024). Western Blot for Anti-V5...and in ternary complexes with other proteins, establishing CRBNmidi as a promising tool for future structure-guided...

  13. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ... typhimurium, S. ordonez, Serratia marcescens, Shigella spp., Vibrio cholerae, Xanthomonas campestris ...10.1111/1574-6968.12241  Keen, N. T., Tamaki, S., Kobayashi, D., & Trollinger, D. (1988). Improved broad-host-range...

  14. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...difficult to treat in the future. Patients not finishing the whole course of antibiotics prescribed to ... cleanliness and personal hygiene standards. Handwashing is an essential component of preventing infection...

  15. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F (2014) Genome-scale transcriptional...Mammalian Protein Complex Structure, Function, and Membership Using Genome-Scale Fitness Screens. Cell Systems...

  17. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...are listed below: EMSA (Electrophoretic Mobility Shift Assays) ELISA (Enzyme-Linked Immunosorbent Assay...Biotinylated probes in the electrophoretic mobility shift assay to examine specific dsDNA, ssDNA or RNA–protein...

  18. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...of a problem when the excitation wavelength is shifted to longer wavelengths. Therefore, the use of bright...25831507 PubMed Central PMCID: PMC4394297. 16. Iwano, Satoshi, et al. "Single-cell bioluminescence imaging of...

  19. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...year, Russell and Hirata from the University of Washington reported a surprising finding - when delivered...effective for introducing non-sequence specific frameshift mutations through aberrant double strand break...

  20. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...it. The other half of each day you can spend accomplishing small tasks (like... preparing plasmids to send... Send out as many emails as possible While accomplishing any one of these things won’t mark an end to...
Showing: 301 - 320 of 536 results