Skip to main content

We narrowed to 665 results for: SIM

Showing: 301 - 320 of 665 results
  1. The MTA at Addgene

    Type
    Blog Post
    ...agreement, read on!  What is the MTA?  An MTA is simply a contract or agreement that states the terms of...clearly communicated. And that’s where MTAs, and similar agreements, shine.  These types of agreements allow...
  2. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ... you have something to ask or say. It can be as simple as “it was nice meeting you.” Include follow-up...person on LinkedIn I once helped by answering one simple question so I accepted the connection invitation...
  3. Components of CRISPR/Cas9

    Type
    Blog Post
    ...using CRISPR/Cas9 for genome editing, researchers simply need to express a gRNA designed to direct Cas9 ...these arrays, they did not know their functions and simply called the repeated elements “direct repeats” and...
  4. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...strong collaborative research team motivated by similar visions and goals? We continue our conversation...and a friend. George Church runs his lab fairly similarly to his advisor Wally Gilbert: “Very interdisciplinary...
  5. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...can either express the plasmid DNA transiently (similar to bacteria) or incorporate the genetic material.... Positive selection in mammalian cells works similarly to that in bacteria and a table of the most commonly...
  6. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...management, and how does it relate to science? In the simplest of terms, a software product manager helps ensure...could code the best operations software possible. Similarly, when the developers needed to know information...
  7. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ..., where multiple fluorescent tools are used simultaneously to monitor or control different cell types ...4.0 International license. Figure 5: A) Simultaneous fluorescence (upper trace) and electrophysiological...
  8. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...Packaging using 293T cells This method is very similar to lentiviral packaging methods. The three components...constructs. Why do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone...
  9. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ... an advanced search option. For the former, you simply type in identifier keywords (Cas name, RefSeq ID...phylogenetic nomenclature and discover orthologs with similar properties (Makarova et al). CasPEDIA is an evolving...
  10. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...organisms. This is often cDNA, shRNA, or something similar, and is contained on the transfer plasmid. If expression...different from gamma-retrovirus, but they perform very similar functions to infiltrate the host cell and deposit...
  11. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...this post in the graphic below - including some simple comparisons. The easy multiplexing afforded by...integrated RNA and CRISPR/Cas toolkit in human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. ...
  12. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...experiences. If you’re hearing multiple people with similar misconceptions, it’s time to rework your strategy...group was just really tired and needed a slower, simpler presentation than I had planned; other times students...
  13. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...variables (scFvs) are ~27kD engineered fusion proteins similar to a Fab fragment but created through engineering...different antigen; binding of each can be either simultaneous or independent of the other (Shahied, 2004)....
  14. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...successfully used in conditions that are the most similar to yours. Take a look at the publications cited...application, a publication successfully used it in a similar way as you plan to. To secondary or not to secondary...
Showing: 301 - 320 of 665 results