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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 661 results for: SIM

Showing: 301 - 320 of 661 results

  1. Components of CRISPR/Cas9

    Type
    Blog Post
    ...using CRISPR/Cas9 for genome editing, researchers simply need to express a gRNA designed to direct Cas9 ...these arrays, they did not know their functions and simply called the repeated elements “direct repeats” and...

  3. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...strong collaborative research team motivated by similar visions and goals? We continue our conversation...and a friend. George Church runs his lab fairly similarly to his advisor Wally Gilbert: “Very interdisciplinary...

  4. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...can either express the plasmid DNA transiently (similar to bacteria) or incorporate the genetic material.... Positive selection in mammalian cells works similarly to that in bacteria and a table of the most commonly...

  5. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...management, and how does it relate to science? In the simplest of terms, a software product manager helps ensure...could code the best operations software possible. Similarly, when the developers needed to know information...

  8. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ... an advanced search option. For the former, you simply type in identifier keywords (Cas name, RefSeq ID...phylogenetic nomenclature and discover orthologs with similar properties (Makarova et al). CasPEDIA is an evolving...

  9. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...organisms. This is often cDNA, shRNA, or something similar, and is contained on the transfer plasmid. If expression...different from gamma-retrovirus, but they perform very similar functions to infiltrate the host cell and deposit...

  10. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ..., where multiple fluorescent tools are used simultaneously to monitor or control different cell types ...4.0 International license. Figure 5: A) Simultaneous fluorescence (upper trace) and electrophysiological...

  11. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...Packaging using 293T cells This method is very similar to lentiviral packaging methods. The three components...constructs. Why do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone...

  13. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...this post in the graphic below - including some simple comparisons. The easy multiplexing afforded by...integrated RNA and CRISPR/Cas toolkit in human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. ...

  15. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...Assembly, Golden Gate etc.), and try to find the simplest way to do it (i.e. avoid assembling too many fragments...help you at this stage and that can be used to simulate in silico cloning. This will definitely help in...

  17. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...experiences. If you’re hearing multiple people with similar misconceptions, it’s time to rework your strategy...group was just really tired and needed a slower, simpler presentation than I had planned; other times students...

  18. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...variables (scFvs) are ~27kD engineered fusion proteins similar to a Fab fragment but created through engineering...different antigen; binding of each can be either simultaneous or independent of the other (Shahied, 2004)....

  19. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...successfully used in conditions that are the most similar to yours. Take a look at the publications cited...application, a publication successfully used it in a similar way as you plan to. To secondary or not to secondary...

  20. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...conjugated in the lab. In the indirect approach, you can simply keep a stock of conjugated secondary antibodies...regardless of the number of protein targets, simplifying multiplex assays.  A brief note Since the indirect...
Showing: 301 - 320 of 661 results