We narrowed to 1,115 results for: STI

... a summer role as a Research Scientist at the Institute of Cancer Research in Sutton, located on the edge...pediatric kidney cancer. This was particularly interesting as I had just completed my thesis on the study...tend to pay better. I worked in a Microbiology testing facility, where I tested materials for bacterial...bacterial contamination. It was a really interesting job at first, everything was very new, and everything was...job received many applications, employers were requesting that only scientists with an MSc or higher apply...to help cover the costs. My MSc courses were interesting and luckily genetics is a sector which has been...been rapidly growing. These factors left me optimistic that I would find a genetics related role soon ...

...seems captivated. Expertly navigate questions Answering questions is arguably the most difficult part ...Anticipate questions: When practicing with your colleagues, ask them to brainstorm potential questions you may...presentation veteran, but have difficulty answering questions. Or perhaps you’re simply attending, but uncertain...constructive criticism, and ask thought-provoking questions. For example, if you’re a virologist presenting...thinking about discussing a figure, jot down the question you were trying to answer, the method you used...your time: We often get so nervous when asked a question that we don’t fully hear or understand what we...have many times answered a completely different question than the intended one. Take a deep breath, and...

...cap: institutions of higher education nonprofit entities related to or affiliated with institutions of ...Having a lead role or critical role at a prestigious institution: This criterion is consistently under scrutiny...exempt from the cap if the applicant works at institutions of higher education, a related or affiliated...would not have been possible. Display of work in artistic exhibitions or showcases (not usually applicable...Proactivity is very important in this case.  You can still assist your PI in the peer review process, but remember...to self-petition. You need the support of your institute to do this one. Universities will usually only...professors. Casseus Law has seen that research institutions are willing to sponsor postdoctoral researchers...

... placing the dipstick into a wash solution removes contaminants while nucleic acids stick around. Finally...requirement for lab equipment. Plus they create plastic waste. Kits can also have the annoying tendency... bonds with water molecules and coaxes it into sticking to the hydrophobic glass filter. Each glass filter...commercial kits, but in less time. The authors estimate that a gravity-based column plasmid maxiprep kit...slurry that has a milk-like appearance. To make DNA stick to the glass particles in glass milk, gel slices...solubilizes both DNA and agarose, and 2) it helps DNA stick to the glass. After binding DNA, the glass particles...detergent, and ball bearings. Then a cellulose dipstick is dipped into this mixture to absorb the nucleic...

...substrates and the ACP and SFP Synthases. Users may still generate their own fluorescent or non-fluorescent...

...Content last reviewed: 23 October 2025 Do you have suggestions for other plasmids that should be added to this...

... pCAG-T7-TALEN(Sangamo)-Destination series, pCAG-Golden-Gate-Esp3I-Destination Takashi Yamamoto pcDNA-TAL-NC2...they used CRISPR to create OsTIR1-expressing cell lines by inserting OsTIR1 into the "safe harbor" AAVS1...Recent studies carried out by Sebastian Lourido and colleagues constitute an important step toward understanding...Alexander Stark and colleagues at the Research Institute of Molecular Pathology have developed an alternative...publication, Kroeze et al. describe their enhanced TANGO arrestin recruitment assay incorporating 314 codon-optimized...bacteria, the process of cloning, combining, and testing these different parts can be quite arduous. The...your gene of interest and cloned into provided destination vectors using low-cost, one-pot golden gate cloning...

...ordering food - everything is covered.   So why are we still printing and gluing pieces of paper in notebooks... needs The familiar paper-based lab notebook is still a standard approach to record-keeping in many labs...In their ideal form, ELNs provide users with a holistic means to manage their work, combining data, team...researchers. The full story is available here. Our questions focused on researchers’ current practices, their...maintenance and support.” Besides that, academic institutions tend to think about the long term, so they need... once they establish the system within their institution. In the study, respondents were also asked about... knowledge of electronic lab notebooks into 9 questions that will help you start and define what exactly...

... order.  Still have questions? Visit the Addgene Help Center If you have additional questions about our... my order? All of these questions are really getting at one bigger question: How does a customer place...answers to other ordering questions here. In addition to answers to common questions about the ordering process... discussing some of our most frequently asked questions related to the ordering process: I placed an... purchase order, which is an official document listing materials and pricing information that is sent ...repository. We distribute it on behalf of depositing institutions. Addgene cannot fulfill orders based on PO documents...

... target recruitment and nicking, producing STITCHR. STITCHR can make a wide variety of genomic edits —...recombinant antibodies for Glypicans DNA editing with STITCHR Dynamic protein labeling with TurboCas Enhancer-AAV...researchers' favorite features and limitations of existing backbones, we're excited to share our very own... embryonic development and cancer. But there is still much to learn about their myriad functions and therapeutic... therapeutic potential. Our partners at the Institute for Protein Innovation (IPI) recently released a...-guided genome editing, think beyond Cas9 with STITCHR: a combined CRISPR / retrotransposon system with...added to the genome (Figure 4). Figure 4: STITCHR consists of a nicking Cas9 fused to a shortened...

... a function of digestion time. For phage DNA that is refractory to enzymatic digestion, however, a physical...diverse biological entities on our planet, but we still know collectively little about how they infect and...RBPs, the host recognition factors of phages) constitute one of the most popular classes of phage proteins... the process of capitalizing on phage RBPs as diagnostic tools and therapeutics. In addition, the strategic...recognize on a host cell. To make matters even more interesting, much of what a phage faces as it approaches ...library of phage DNA This first step involves digesting phage genomic DNA into 1-3 kb (approximately gene-sized...units, which can be purchased cheaply, are small plastic containers that can easily be attached with a hose...

...at allencell.org and the Allen Institute website at alleninstitute.org. A classic challenge in cell biology...at allencell.org and the Allen Institute website at alleninstitute.org. References 1. Roberts, Brock, ...contributed by the gene editing team at the Allen Institute for Cell Science. Learn more by visiting the Allen...large tags into a precise location in the genome is still inefficient, particularly in human cell lines. Furthermore...genomic tagging experiments. Browse the Allen Institute for Cell Science Plasmid Collection Fluorescent...edited hiPS cell lines generated using the Allen Institute for Cell Science Plasmid Collection, please visit...Many thanks to our guest bloggers from the Allen Institute for Cell Science. This post was contributed by...

... do during your training is meet interesting people doing interesting things. I call this building relationships...will be asking the questions, so be prepared. There are many good lists of questions on the internet. To...learning new things from other scientists doing interesting work. Consider this to be like any other research...mind that this is a good time to ask personal questions about salary, opportunities for advancement, challenges...challenges and disappointments. These more personal questions can elicit the most valuable insights you will...people like to help One good place to look for interesting connections is your university’s alumni database...too busy to respond to you). You will uncover interesting opportunities by meeting with people working ...

...last week.  This fantastic city-wide effort was led by two amazing postdocs, Kristin Krukenberg and Jessica...oft quoted statistic that only 15% of current postdocs will be academic principal investigators. She also...for science—we have powerful tools, beautiful questions, and smart scientists.  At the same time, he pointed...He charged the postdocs to “Grab ahold of your destinies” and also suggested some concrete actions that...audience to use their useful energy and to choose institutions with allies engaged in training excellence to...action Each of the speakers in the panel had interesting words of experience and encouragement.  Dr. Sibby... training programs (like at the Keck Graduate Institute), funding for training (such as the NIH BEST award...

...Cre-dependent Flp-dependent Cre and Flp-dependent Constitutive (non-cre dependent) Serotype AAV1 AAV2 AAV5 ...

...end of your analysis process. You’ll still need to do statistical analysis to determine if there are any... differences of twofold or greater with good statistical significance (Taylor, 2017). With the right conditions...some general helpful principles (and I recommend Bustin, 2017 for a more in-depth review.)  Your amplicon...different efficacies DART-PCR No Raw Data Statistically defined log-linear phase   Table 2: ... them back before reporting the data!   Statistical analysis  Getting your RQ value isn’t the end... Yuan, 2006 has a good overview of different statistical analysis methods that pair well with slightly...step, remember we here at Addgene frown upon statistical laxness in all its forms. Reporting your data...

...outstanding questions you have, well before experimental crunch time. Don’t: Wait to ask questions and/or ...already read a paper or protocol. If you have questions while reading these materials (maybe a step in...then you’ve done previously) you can bring those questions to your training! All of these things will help...physically where relevant) and identify any gaps or questions you have, then bring them to your mentor. Pro...They can then plan accordingly in case you have questions or need a little assistance at some point during...yours is! For instance, some people like to ask questions throughout training, while others prefer to review...notes, process what they’ve just learned, and ask questions later – maybe even the next day. In the latter...

...first process by simply copying and pasting pieces from our existing script into the script block of the...several sequential computational tasks for which existing script(s) have been developed for a singular purpose...looking for was the ability to re-use or borrow existing code and control how the various pieces were connected...and optimize our pipeline. After reviewing and testing several platforms for engineering pipelines, we...resources. Now, we could iterate our development and testing cycle faster due to the ease of jumping back and...sequences on a 96-well plate basis, but for rapid testing and refinements, we specified a select small number...time and connect each subsequent process to the existing ones. Once our first process was working as expected...

...nucleic acid detection is crucial for clinical diagnostics, genotyping, and biotechnological advancements...DETECTR demonstrate the power of CRISPR-based diagnostics. SHERLOCK- Specific High Sensitivity Enzymatic...lab demonstrated that SHERLOCK could reliably distinguish between Zika and a closely-related virus, Dengue... an array of applications including clinical diagnostics (e.g. pathogen or virus detection), therapeutics...purified from patient samples and can be read using a dipstick in under one hour. While the lab points out that... protocol serves as a platform for advancing diagnostics.     DETECTR- DNA Endonuclease Targeted CRISPR...lab demonstrated that DETECTR could accurately distinguish between two similar types of HPV, HPV16 and HPV18...

...vector systems. With the appropriate entry and destination vectors, one can use Gateway to clone a gene ...generated entry clone and the attR sites of the destination vector. This reaction is catalyzed by the LR ...containing the ccdB gene is excised from the destination vector.  Once the BP and/or LR reactions are ...select the positive clones. The entry clone and destination vector carry different antibiotic resistance ...gene is present in the donor vectors and the destination vectors prior to recombination, and it is exchanged...expression clone, it is important to choose the destination vector that best fits your experiment. This choice...doxycycline-inducible pLIX_403. The chosen attR destination vector will recombine with the attL-entry clone...