We narrowed to 472 results for: Sele

1. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   Blog Post

   ...and tsPurple chromoproteins to create negative selection markers for allelic exchange. These vectors contain...

2. Truncated gRNAs for Regulating Gene Expression

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   ...challenge of attempting to simultaneously and selectively induce both cutting and regulation of expression...

3. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   ...started, too much time for this project was spent selecting guides, designing primers, and running analyses...

4. Addgene's Cancer Collection Pages Connect the Research Community

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   ... Plasmids and Resources page, investigators may select a pathway and identify any genes within that pathway...

5. Google Forums Round Up: First Impressions of NgAgo

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   ... Addgene. Addgene performs Sanger sequencing on select regions of all distributed plasmids as part of ...

6. PITChing MMEJ as an Alternative Route for Gene Editing

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   ...and the PITCh- and locus-specific gRNAs Step 4: Select for puromycin resistant cells Step 5: PCR amplify...

7. Light Sheet Fluorescence Microscopy

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   ...Stelzer’s group described the single-plane or selective-plane illumination microscope (SPIM). In order...

8. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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   ... a fitness cost and are eliminated by natural selection, gene drives distort inheritance in their favor...

9. Lentivirus Plasmids

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   ...expression of shRNA; puromycin selection. See plasmid 21916 for Neo selection. Wiederschain 11578 pSico 3rd...with EGFP marker. See plasmid 14749 for Thy1.1 selection. Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible...shRNAs or miRNAs, and use a wide variety of drug selection markers. See article for more variations. Campeau...-puro 3rd CMV driven expression of cDNA. Puro selection. Shih 25737 pSLIK-Hygro 3rd Tet-based inducible...Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin selection. See plasmid 21915 for Puro resistance. Wiederschain...

10. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    ...Best Way to Elute and Store Your Plasmid DNA? Selecting Your Plasmid Purification Kit Resources on Addgene.org...

11. Course-Based Undergraduate Research Experiences (CUREs)

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    ...   Fig. 2: Students in an MDH CURE replaced select residues of human mitochondrial MDH (hMDH2) with...

12. AAVs CREATed for Gene Delivery to the CNS and PNS

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    ..., Pravdo PL, Simpson BP, et al. Cre-dependent selection yields AAV variants for widespread gene transfer...

13. Simplify Cloning with in vivo Assembly

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    ...specifically cuts methylated DNA, so it will selectively destroy the template, but not newly synthesized...

14. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...Fusing FP fragments to your PIPs requires careful selection of FP fragments and, preferably, some information...

15. Summer SciComm Series: Modes of Communication

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    ...start can save a lot of trouble in the long run.  Selecting the method Once you’ve answered the above questions...

16. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    ...linearly distant regions close together. gRNA Selection and Filtering for Off-target Effects We first ...

17. Plasmids 101: Colony PCR

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    ...make sure to use the appropriate antibiotic for selection.   Lysing bacteria and setting up PCR reactions...

18. CRISPR Antimicrobials

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    ...resistant bacteria, and inappropriate antibiotic usage selects for resistant bacteria, allowing them to multiply...

19. Tips for Screening with Yeast Two Hybrid Systems

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    ... histidine-auxotrophic host strains are used to select for binding-dependent expression of the HIS3 gene...

20. CRISPR Plasmids - Single-Strand Break (Nick)

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    ...Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI... Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI ...Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions...