We narrowed to 466 results for: Sele
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TypeBlog Post... resources on the Addgene blog Antibodies 101:Selecting the Right AntibodyAntibodies 101: Secondary AntibodiesAntibodies...
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Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...and tsPurple chromoproteins to create negative selection markers for allelic exchange. These vectors contain... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog Post... Plasmids and Resources page, investigators may select a pathway and identify any genes within that pathway... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post... Addgene. Addgene performs Sanger sequencing on select regions of all distributed plasmids as part of ... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post...and the PITCh- and locus-specific gRNAs Step 4: Select for puromycin resistant cells Step 5: PCR amplify... -
Lentivirus Plasmids
TypeCollection...expression of shRNA; puromycin selection. See plasmid 21916 for Neo selection. Wiederschain 11578 pSico 3rd...with EGFP marker. See plasmid 14749 for Thy1.1 selection. Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible...shRNAs or miRNAs, and use a wide variety of drug selection markers. See article for more variations. Campeau...-puro 3rd CMV driven expression of cDNA. Puro selection. Shih 25737 pSLIK-Hygro 3rd Tet-based inducible...Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin selection. See plasmid 21915 for Puro resistance. Wiederschain... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post... histidine-auxotrophic host strains are used to select for binding-dependent expression of the HIS3 gene... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...specifically cuts methylated DNA, so it will selectively destroy the template, but not newly synthesized... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...Fusing FP fragments to your PIPs requires careful selection of FP fragments and, preferably, some information... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post... Fig. 2: Students in an MDH CURE replaced select residues of human mitochondrial MDH (hMDH2) with... -
Summer SciComm Series: Modes of Communication
TypeBlog Post...start can save a lot of trouble in the long run. Selecting the method Once you’ve answered the above questions... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post..., Pravdo PL, Simpson BP, et al. Cre-dependent selection yields AAV variants for widespread gene transfer... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...linearly distant regions close together. gRNA Selection and Filtering for Off-target Effects We first ... -
Plasmids 101: Colony PCR
TypeBlog Post...make sure to use the appropriate antibiotic for selection. Lysing bacteria and setting up PCR reactions... -
CRISPR Antimicrobials
TypeBlog Post...resistant bacteria, and inappropriate antibiotic usage selects for resistant bacteria, allowing them to multiply... -
CRISPR Plasmids - Single-Strand Break (Nick)
TypeCollection...Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI... Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI ...Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions... -
CRISPR Plasmids - Base Edit
TypeCollection...Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI... Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI ...Publication Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...someone other than the actual author. Instead, selected images are said to be representative or the statistical... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...research for single or multiple disciplines – you can select the disciplines and the years. Which leads to the... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...balanced with predicted off-target activity when selecting guides. Guides were delivered as a pooled library...