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Showing: 301 - 320 of 576 results

  1. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...study the effect of gene knockout in vivo and in vitro, as well as to insert targeted mutations through...gRNAs may be used to target genes with overlapping activity. Multiple gRNAs can also facilitate deletions ...

  3. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...skills such as critical thinking, decision making, creativity, or data analysis. Conveniently, these are all... and taking on independent projects, you will inevitably develop problem solving skills passively. But...

  5. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies are produced...staining of tissue because they have limited cross-reactivity with commonly used anti-rodent or -rabbit secondary...

  7. dTAG - You're it!

    Type
    Blog Post
    ...depleting a protein-of-interest, increasing target selectivity, overcoming resistance to inhibitors, and inducing...performance, great depth of degradation, absolute selectivity, broad utility for degrading targets in different...

  9. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...important implications for the fusion protein effectivity), and a fluorescent protein tag for visualization...VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair pathways, Amit Choudhary's...

  12. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...provides a noninvasive method for manipulating cell activity, especially neuronal firing. What is chemogenetics...Stone O, Roth BL, Jin J (2015) The First Structure–Activity Relationship Studies for Designer Receptors Exclusively...

  13. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...could in theory promote off-target binding. The activity of SpRY was validated in double stranded cleavage...assessment of the PAM compatibility and editing activities of Cas9 variants. Nucleic Acids Res. 2021 Sep...

  14. Hot Plasmids - October 2020

    Type
    Blog Post
    ...researchers can explore synaptic integration and connectivity without the constraints of antibody labeling...exclusively in huge bacteriophage genomes. It’s active in vitro and in human and plant cells. Its small size offers...

  16. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...polyadenylation generally serves to promote transcript longevity in eukaryotes and promote transcript degradation...virus 40 late polyadenylation signal by using in vitro analyses. Molecular and Cellular Biology. 1992. ...

  18. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...bead assay (Figure 2). After finding no cross-reactivity, antibody candidates underwent affinity screening... that recognizes a NNGG PAM and has comparable activity to SaCas9. A new yeast inducible CRISPRi library...
Showing: 301 - 320 of 576 results