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We narrowed to 451 results for: abo.5

Showing: 301 - 320 of 451 results

  1. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...Retroviruses and cancer. Rev Infect Dis. 1982 Sep-Oct;4(5):1015-25. DOI: 10.1093/clinids/4.5.1015 Lee C.S., ...clinical settings. AAV is also a cool tool in the laboratory! It is ideal for whole animal administration,... be helpful to you. Interested in learning more about a specific virus? We have you covered with guides..., adenovirus, and AAV. If you’re wondering more about what applications these viruses are good for, we...

  2. 7 Tips to Secure a STEAM Internship This Summer

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    Blog Post
    ...will end up with a stellar letter of support.        5. Application submission  Set a calendar reminder for...Rose is passionate about outreach among low-income communities to educate parents about resources that can...automated filters of consideration. If you are worried about any eligibility provision, don’t guess. Simply reach...

  3. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    Blog Post
    ...phase, the number of plasmids per cell increases 4-5 fold (for pUC plasmids) or ~2 fold (for p15a, R6K,...is useful for many metabolic engineering and synthetic biology goals. In metabolic engineering, the expression...or can interfere with the precision needed in metabolic engineering or other synthetic biology circuits...

  4. Visualizing Protein Turnover In Situ

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    Blog Post
    ...PMID: 25775042. PubMed Central PMCID: PMC4414919. 5. Yarmolinsky, Michael B., and L. Gabriel. "Inhibition... FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid, azidohomoalanine...both quantitative and spatio-temporal information about the turnover of specific endogenous proteins in ... interested in neurobiology.  She is passionate about communicating science to all audiences. Right now...

  5. Plasmids 101: The protein expression toolbox

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    Blog Post
    ...degradation. Nature Chemical Biology. May 2018. 14(5): 431-441. PubMed PMID: 29581585. Additional resources...which can be used with this approach - read more about them on our CRISPRa blog! Fig. 1 CRISPR ... is a lot of overlap with the systems discussed above, just with more elements of control. Tet promoters...to be site-specifically knocked in. This can be labor intensive and time consuming. If your native protein...

  6. Antibodies 101: Polyclonal Antibodies

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    Blog Post
    ...for your species. Affinity purification Only up to 5% of the IgG antibodies produced by an animal are specific...  Read about another common type of antibody for research, monoclonal antibodies Learn about the advantages...Once the initial antibody response starts to wane about a month later, the animal is given additional booster...

  7. Top Requested Lentivirus and AAV of 2016

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    Blog Post
    ...tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse brain. PLoS One. 2013 Sep...Resources on the Addgene Blog Learn More About Lentivirus Learn more About AAV Get the Scoop on Our Viral Service...

  8. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post
    ...mechanism, but their small packaging capacity of 4-5 kb limits the delivery of larger transgenes, such ... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...tracing using the transgenic zebrafish model, ubi:zebrabow. In this system, cells express various fluorescent...

  9. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ... are safe! Colony screening Pick a few colonies (5-10) and grow them in a small culture volume (2 or ...they get old, they lose competency). If all the above is correct, you should probably have a second and...blog posts Read blog posts on plasmid cloning Read about different plasmid elements Resources on Addgene.org...

  10. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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    Blog Post
    ...were never stored on dry ice/exposed to CO2 (Fig. 5). Basically, the CO2 goes in and the CO2 goes out....return the sample back to the original pH. What about putting it back in the freezer? We then tested whether...

  11. OCaMP: A New Calcium Indicator for Neural Imaging

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    Blog Post
    ...CC-BY-NC-ND 4.0 International license. Figure 5: A) Simultaneous fluorescence (upper trace) and electrophysiological... view of traces corresponding to the dashed box above. The number of action potential (AP) spiking events...

  12. CasPEDIA: A Functional Classification of Cas Enzymes

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    Blog Post
    ...as well as their orientation relative to the gRNA (5’ or 3’). In some cases, there is no requirement, which...you are a structure junky who just wants to learn about Cas domains. CasPEDIA either directly has this information...

  13. Optogenetics + CRISPR, Using Light to Control Genome Editing

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    Blog Post
    ...DNA damage. Far-red light, however, can penetrate 5 mm past the surface of skin and is less phototoxic...633–639 . https://doi.org/10.1038/s41592-019-0436-5 Mathony J, Hoffmann MD, Niopek D (2020) Optogenetics...of the genome editing process. Read on to learn about the various light-controlled CRISPR tools available...reaching the amber stop codon, TAG. (To learn more about site-specific incorporation of amino acids using...

  14. 22 Hot Plasmid Technologies from 2014

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    Blog Post
    ...Synth Biol 2014. Feb 5 (Web); DOI: 10.1021/sb4001504. For further reading about Nicola Patron's MoClo.... Steinfeld et al., ACS Chem Biol 2014 May 16;9(5):1104-12. Heinemann et al., FEBS Lett 2012 Oct 19...creating a single transcriptional unit (Ex: a promoter, 5’UTR, coding region, and terminator). Next, up to six...2012 Mar 20;109(12):E690-7. For further reading about SpyLigase technology, read the Addgene interview...in optimizing TALEN assembly and activity? The laboratory of Takashi Yamamoto has created a complete TALEN...pGreen-IIS based destination vectors. To learn more about the GreenGate cloning system, see the detailed plasmid...

  15. Viral Vectors 101: Systemic Capsids

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    Blog Post
    ...administered at a total dose between 1 x 1011 and 5 x 1011 vg per animal (i.e., 10 and 50 µL of vector...across the Blood-Brain Barrier. Molecular Therapy, 27(5), 912–921. https://doi.org/10.1016/j.ymthe.2019.02.013...targeting distinct brain cell types. Nature Methods, 17(5), 541–550. https://doi.org/10.1038/s41592-020-0799... for their use. Then, we’ll provide information about commonly used and emerging capsids to help you decide...our Caltech Systemic Capsids page to learn more about that collection. Table 1: Systemic capsid plasmids...slices Several of the systemic capsids mentioned above have also been tested on ex vivo brain slices obtained...

  16. Developing Lab Management Software for Biology

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    Blog Post
    ...situations where you learn biology terms (plasmid, 5-prime, CRISPR, growth strains, vector backbone, etc...you access to hard data about what steps are most likely to fail. Our laboratory manager would definitely...can be (there will always be confusion about this though) about the fact that containers are distinct ... Laboratory management software is not a requisite for a functioning lab, but it is for a scaleable lab...techs through the process. The problem? As we said above, any one of these steps can fail but, in our current...gooey contents of said containers, think carefully about how you want your software to treat those encoded...this case, the physical considerations of the laboratory make object composition a smarter choice than...

  17. Seven Tips for Using LinkedIn as a Scientist

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    Blog Post
    ... inquiry, you always want to read before sending. 5) You can use LinkedIn before an interview, but be ...include a note about where you met IRL (in real life) and perhaps what you talked about (or how good the... this rule, but not that many). I’m asked a lot about requesting connections to complete strangers for...have been able to tell when some start-ups were about to be bought by seeing certain groups of people ...using social media as a scientists Read blog posts about networking ...

  18. How to Prepare for an Industry Interview

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    Blog Post
    ...place for drafting my stories. In total, I prepare 5-6 stories for an interview, since one story usually...you do to get ready for this interview? Maybe the above scenario sounds familiar to you. It was my experience...positions often required a one-hour presentation about a prior research project. In total, the interviews... find lists that have lots of general questions about job responsibilities, performance evaluations, professional...require. Frequently they will ask you to present about your prior research. This is a chance to highlight...

  19. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    Blog Post
    ...linear equation (remember y = mx+b?). Figure 5: A standard curve is generated by plotting OD against...blog Learn about the advantages and disadvantages of using monoclonal antibodies. Read about other techniques... require two different antibodies. As mentioned above, it can also be used to characterize antibodies ...concentration of proteins in an ELISA well As mentioned above, the measurement of the concentration of proteins...

  20. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    Blog Post
    ...Curr Protoc Microbiol. (2013); Chapter 14:Unit14D.5. PubMed PMID: 23686825. PubMed Central PMCID: PMC4135440...general consideration for behavioral testing in a laboratory, a high level of experimental control is required... after stereotaxic injection. Find more details about choosing AAV or lentiviral vectors in our previous...it’s crucial to appropriately time the injection about two weeks before the behavior test. This time window... Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...
Showing: 301 - 320 of 451 results