Addgene

We narrowed to 336 results for: ada

Showing: 301 - 320 of 336 results

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Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

Type

Blog Post

...Genetic compensation triggered by mutant mRNA degradation." Nature 568.7751 (2019): 193. PubMed PMID: 30944477...
Antibodies 101: The Four ELISAs and When to Use Them

Type

Blog Post

...measure antibody levels, scientists have since adapted it for a host of different proteins and small molecules...
Plasmids 101: Stringent Regulation of Replication

Type

Blog Post

...Figure 2: Prokaryotic chromosomal replication. Adapted from BioRender template. Since DnaA binding...
Plasmids 101: Broad Host Range Plasmids

Type

Blog Post

...contains structural elements that are versatile and adaptable. Variation in ori structure allows plasmids to...
An Inside Look into Addgene's Dev Team

Type

Blog Post

...in iterative chunks, we have the flexibility to adapt and easily respond to changing needs of the teams...
Plasmids 101: Codon usage bias

Type

Blog Post

...structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms often need...
CRISPR Activation: A Practical Guide

Type

Blog Post

...doi.org/10.1038/nmeth.3871 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes...
Adeno-associated Viruses (AAVs) for Genome Editing

Type

Blog Post

...and pAAV-TK-Acceptor) so that they can be easily adapted to edit your gene of choice. These vectors are ...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

...that a fusion protein is cleaved by proteases. Nowadays, with ample structural information available, ...
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Type

Blog Post

... visualization of two colors. Similarly, Flybow adapts Brainbow 2.0 for use in Drosophila melanogaster...
Plasmids 101: Biotinylation

Type

Blog Post

.... The biotin-avidin interaction has also been adapted for use in immunohistochemistry and antibody labelling...
Five Popular Model Organisms, Part 2

Type

Blog Post

...plant species and metabolic properties affecting adaptations. Arabidopsis thaliana flowers. Image from...
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Type

Blog Post

...solution is to include an in-frame degron, or degradation sequence, upstream of the 5’ splice donor and...
Quick Guide to Near-Infrared Fluorescent Proteins

Type

Blog Post

... changes occurring due to protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova ...
Antibodies 101: Normalization and Loading Controls for Western Blots

Type

Blog Post

...the gel (n=3). Data is presented as mean ± SEM. Adapted from Analytical Biochemistry, vol 440, Gilda, J...
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Type

Blog Post

...parts. Gibson assembly meets CRISPR Gibson can be adapted to more complicated cloning schemes, such as those...
Treating Muscular Dystrophy with CRISPR Gene Editing

Type

Blog Post

...low-frequency indels will not cause frameshifts. Figure adapted from Osterout et al. (2015b) under a CC-BY 4.0 ...
Technologies Enabled by NanoLuc® Luciferase

Type

Blog Post

...accuators. The researchers took a FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase...
Viral Vectors 101: Optogenetic Tools

Type

Blog Post

...10.1016/j.sbi.2019.05.006 Vierock, J., Rodriguez-Rozada, S., Dieter, A., Pieper, F., Sims, R., Tenedini...
Viral Vectors 101: AAV Variables That Matter

Type

Blog Post

...Brans, M. A. D., Luijendijk, M. C., Garner, K. M., & Adan, R. A. H. (2010). Optimization of Adeno-Associated...

Showing: 301 - 320 of 336 results

Results per page:

Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

Antibodies 101: The Four ELISAs and When to Use Them

Plasmids 101: Stringent Regulation of Replication

Plasmids 101: Broad Host Range Plasmids

An Inside Look into Addgene's Dev Team

Plasmids 101: Codon usage bias

CRISPR Activation: A Practical Guide

Adeno-associated Viruses (AAVs) for Genome Editing

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Plasmids 101: Biotinylation

Five Popular Model Organisms, Part 2

Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Quick Guide to Near-Infrared Fluorescent Proteins

Antibodies 101: Normalization and Loading Controls for Western Blots

Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Treating Muscular Dystrophy with CRISPR Gene Editing

Technologies Enabled by NanoLuc® Luciferase

Viral Vectors 101: Optogenetic Tools

Viral Vectors 101: AAV Variables That Matter

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