We narrowed to 344 results for: ada

1. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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   Blog Post

   ...Genetic compensation triggered by mutant mRNA degradation." Nature 568.7751 (2019): 193. PubMed PMID: 30944477...

2. CRISPR Activation: A Practical Guide

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   ...doi.org/10.1038/nmeth.3871 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes...

3. Fluorescent Proteins 101: Aptamer Fluorophores

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   ...nucleotide-based ligand binders would prove to be highly adaptable tools. The key to aptamers’ flexibility is their...

4. Mesothelioma - Causes, Symptoms, and Treatment

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   ...like the Asbestos Disease Awareness Organization (ADAO) are working toward such a ban, and the president...

5. Antibiotic Resistance: An Old Solution but a New Problem

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   ...bacteriology and drug resistance.  It seems as though, nowadays, we cannot make it a full month without a new,...

6. Using Video to Share Your Science: We Share Science

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   ...published in scientific journals flies under the radar and gains little notice. A 2009 research study found...

7. Adeno-associated Viruses (AAVs) for Genome Editing

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   ...and pAAV-TK-Acceptor) so that they can be easily adapted to edit your gene of choice. These vectors are ...

8. Plasmids 101: Expressing shRNAs for RNA Interference

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   ...(2021). Life of RISC: Formation, action, and degradation of RNA-induced silencing complex. Molecular Cell...

9. Antibodies 101: Normalization and Loading Controls for Western Blots

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   ...the gel (n=3). Data is presented as mean ± SEM. Adapted from Analytical Biochemistry, vol 440, Gilda, J...

10. Five Popular Model Organisms, Part 2

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    ...plant species and metabolic properties affecting adaptations.  Arabidopsis thaliana flowers. Image from...

11. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    Blog Post

    ...solution is to include an in-frame degron, or degradation sequence, upstream of the 5’ splice donor and...

12. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    ...that a fusion protein is cleaved by proteases. Nowadays, with ample structural information available, ...

13. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    ... visualization of two colors. Similarly, Flybow adapts Brainbow 2.0 for use in Drosophila melanogaster...

14. Plasmids 101: Biotinylation

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    .... The biotin-avidin interaction has also been adapted for use in immunohistochemistry and antibody labelling...

15. Quick Guide to Near-Infrared Fluorescent Proteins

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    ... changes occurring due to protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova ...

16. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    ...parts. Gibson assembly meets CRISPR Gibson can be adapted to more complicated cloning schemes, such as those...

17. Treating Muscular Dystrophy with CRISPR Gene Editing

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    ...low-frequency indels will not cause frameshifts. Figure adapted from Osterout et al. (2015b) under a CC-BY 4.0 ...

18. Technologies Enabled by NanoLuc® Luciferase

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    Blog Post

    ...accuators. The researchers took a FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase...

19. PiggyBac-ing Through the Genome Editing Field

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    .... Jonkers, M. van Lohuizen, A. Berns, and D. J. Adams, “A high-throughput splinkerette-PCR method for ...

20. Viral Vectors 101: Optogenetic Tools

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    ...10.1016/j.sbi.2019.05.006 Vierock, J., Rodriguez-Rozada, S., Dieter, A., Pieper, F., Sims, R., Tenedini...