We narrowed to 333 results for: cki

1. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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   Blog Post

   ...different from how it’s used in other systems. Luckily, you don’t have to look far for plasmids that have...

2. Antibodies 101: Introduction to Immunofluorescence

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   ...permeabilization, antigen retrieval, and sectioning. Blocking Block is used to reduce non-specific binding...

3. Antibodies 101: Single Chain Fragment Variables (scFvs)

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   ... signal is boosted and enables single molecule tracking in living cells. The HA frankenbody is an HA tag...

4. The Fluorescent Vegetables in Aptamer Soup

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   ...bright, high-affinity complex for RNA labeling and tracking." ACS chemical biology 9.10 (2014): 2412-2420....

5. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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   ... so, the WHO recommends one strategy: testing, tracking, and social distancing. With countries like South...

6. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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   ...Surveillance Mechanism That Eliminates Transcripts Lacking Termination Codons. Science 295:2258–2261 . https...

7. CRISPR 101: Off-Target Effects

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   ...enzyme swaps will only reduce off targets where nicking/cleaving of DNA occurs, not for deadCas activator...

8. A Control for All Seasons

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   ...noise. This can be done either by modifying the blocking conditions or by substituting for a more specific...

9. Viral Vectors 101: AAV Variables That Matter

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   ... factors can also affect your AAV experiments. Luckily, the other posts in our Viral Vectors 101 series...

10. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    ...methods section found within a published manuscript. Luckily, many organizations have been working to provide...

11. How to Design Your gRNA for CRISPR Genome Editing

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    ...gRNAs to choose from. With so many possibilities, picking a gRNA with an optimized sequence is of primary...

12. Generating Mouse Models Using CRISPR/Cas9

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    ..., Nakauchi H (2018) Intra-embryo Gene Cassette Knockin by CRISPR/Cas9-Mediated Genome Editing with Adeno-Associated...

13. Building Global Connections with the International Mentorship Program USA-EUROPE

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    Blog Post

    ...2014-15, University of Oviedo): “I couldn’t be luckier with my mentor, since she does research at UK in...

14. Live and Let Dye: Self-Labeling Protein Tags

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    ...time points.   Figure 3: Single-molecule tracking of actin filament assembly-regulating proteins...

15. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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    ...Read about Homology Directed Repair Use Knockout/Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR...

16. When Fidelity Matters: A frank discussion about ligase fidelity

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    ... a method relies on accurate ligation of nicks lacking gaps or mismatched base pairs (as in the case for...

17. Transferable Skills Guide: Teamwork

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    ...yourself. Instead of thinking “What’s my angle on attacking this problem?”, try thinking “Who can I assemble...

18. Finding Your Science Policy Path

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    ...Relationship building is key in science policy. Luckily, there are many mechanisms in place to ensure your...

19. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    ...inversion, and then lox sites excision, therefore locking the opsin into the correct orientation to be transcribed...

20. Antibodies 101: Flow Cytometry

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    ...43:404–409 . https://doi.org/10.1063/1.1685647 McKinnon KM (2018) Flow Cytometry: An Overview. Current...