We narrowed to 334 results for: cki;

1. Getting the Most from Your Lentiviral Transduction

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   Blog Post

   ...lentivirus to be time consuming, difficult, and lacking in reproducibility. Read on for some tips for getting...

2. Plasmids 101: Control Plasmids

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   Blog Post

   ...results can be compared; results from an experiment lacking the proper controls are invariably inconclusive...

3. Data Freedom: The Expansion of Data Sharing in Research Publications

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   Blog Post

   ...Instead of providing a “dead end” figure or table, clicking on the published chart could reveal the underlying...

4. The Fluorescent Vegetables in Aptamer Soup

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   Blog Post

   ...bright, high-affinity complex for RNA labeling and tracking." ACS chemical biology 9.10 (2014): 2412-2420....

5. Antibodies 101: Introduction to Gating in Flow Cytometry

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   Blog Post

   ...whether a tighter or looser gate makes sense. Here, luckily, the vast majority of my cells of interest lies...

6. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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   Blog Post

   ...different from how it’s used in other systems. Luckily, you don’t have to look far for plasmids that have...

7. Antibodies 101: Introduction to Immunofluorescence

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   Blog Post

   ...permeabilization, antigen retrieval, and sectioning. Blocking Block is used to reduce non-specific binding...

8. Antibodies 101: Single Chain Fragment Variables (scFvs)

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   Blog Post

   ... signal is boosted and enables single molecule tracking in living cells. The HA frankenbody is an HA tag...

9. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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   Blog Post

   ...Surveillance Mechanism That Eliminates Transcripts Lacking Termination Codons. Science 295:2258–2261 . https...

10. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    Blog Post

    ... so, the WHO recommends one strategy: testing, tracking, and social distancing. With countries like South...

11. CRISPR 101: Off-Target Effects

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    Blog Post

    ...enzyme swaps will only reduce off targets where nicking/cleaving of DNA occurs, not for deadCas activator...

12. A Control for All Seasons

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    Blog Post

    ...noise. This can be done either by modifying the blocking conditions or by substituting for a more specific...

13. Viral Vectors 101: AAV Variables That Matter

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    Blog Post

    ... factors can also affect your AAV experiments. Luckily, the other posts in our Viral Vectors 101 series...

14. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    Blog Post

    ...methods section found within a published manuscript. Luckily, many organizations have been working to provide...

15. Generating Mouse Models Using CRISPR/Cas9

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    Blog Post

    ..., Nakauchi H (2018) Intra-embryo Gene Cassette Knockin by CRISPR/Cas9-Mediated Genome Editing with Adeno-Associated...

16. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post

    ...gRNAs to choose from. With so many possibilities, picking a gRNA with an optimized sequence is of primary...

17. Building Global Connections with the International Mentorship Program USA-EUROPE

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    Blog Post

    ...2014-15, University of Oviedo): “I couldn’t be luckier with my mentor, since she does research at UK in...

18. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post

    ... a method relies on accurate ligation of nicks lacking gaps or mismatched base pairs (as in the case for...

19. Transferable Skills Guide: Teamwork

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    Blog Post

    ...yourself. Instead of thinking “What’s my angle on attacking this problem?”, try thinking “Who can I assemble...

20. Antibodies 101: Antibody Engineering and Directed Evolution

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    Blog Post

    ...8_2 McMahon, C., Baier, A. S., Pascolutti, R., Wegrecki, M., Zheng, S., Ong, J. X., Erlandson, S. C., ...