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TypeBlog Post... community is generating a massive amount of descriptive transcriptomics data. Custom CRISPR screens designed...videos and text on the website for more detailed descriptions of how to use the software. Here is a brief ...sequences, and more. Output_Compact: A text file that describes which genes were included, and how many gRNAs ...
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CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...like AAV. In addition to the mammalian option described below, plasmids for making polycistronic gRNAs...oligonucleotide assembly. Unlike some of the plasmids described above, this vector does not contain Cas9, so you... are similar to the Csy4 polycistronic system described above, except that they use an endogenous nuclease... -
Congrats to the 2019 Deck The Lab Winners
TypeBlog Post...AnoeskaMoosdijk @Saskia_deMan @SILS_UvA @addgene #SciArt #fluorescence #LCAM #DeckTheLab pic.twitter.com/pYBL3aLK4f...from lab gloves. The tree was adorned with a bioluminescent tree topper, tube “lights,” and autoclave tape... -
Addgene Protocols Go Viral: AAV Purification and Titration
TypeBlog Post...article describing a sucrose supplementation strategy to improve yield. In addition, as described in this... -
Newly Updated AAV Data Hub!
TypeBlog Post...your experiment* Functional data, if the report describes a functional vector such as a channelrhodopsin...site as well as the target region, if the report describes a retrograde serotype An abstract that summarizes... -
Editor's Choice, July 2016
TypeBlog Post...Fessenden at Brigham and Women's Hospital. This post describes how to target and use FRET flurophores for a variety...phenomena and about how fluorophores other than fluorescent proteins can be incredibly useful in FRET experiments... -
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog Post...researchers at Japan’s Hiroshima University who described their new tool in Scientific Reports in June. ... gap. As Sakuma and colleague Takashi Yamamoto describe in their recent study, the construction system... -
Microbes: Look, Listen, and Tell
TypeBlog Post...paper thin and very portable microscope. Less fluorescence admittedly, but still so beautiful! Enabling...agar art have a look at our Microbiology and Fluorescent Protein Resources and get started! I learned... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog Post...activated and can cleave fluorescent probes of dsDNA and RNA in trans. Fluorescent signal indicates the presence... -
Addgene's Top Blog Posts from 2020
TypeBlog Post...service features 210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is...Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate color! They'... -
Editor's Choice, September 2016
TypeBlog Post...Jessica Sacher from the University of Alberta and describes a technique you can use to find phage proteins...molecular biology tool, P1 phage. 2) The post describes an ingenious but simple technique to screen for... -
What's New in CRISPR - September 2019
TypeBlog Post...Cas9 and a donor vector to create knock-ins of fluorescent proteins, fusion proteins, or transcriptional...interest and on either side of the cargo (ex: fluorescent protein gene) on the donor vector. Therefore,... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog Post... Michael Davidson Fluorescent Protein collection, a ‘One-Stop Shop’ for Fluorescent Proteins, and plasmids... -
Working 9-5: an Addgene Co-op
TypeBlog Post...process When I first saw the comprehensive job description for this co-op, I was immediately interested ...had stood out to me because it gave me a full description of the tasks I would be responsible for, in comparison... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog Post... reagents from 20 contributors, including 61 fluorescent probes, representing imaging from 47 different...the Addgene blog Antibodies 101: Multiplex Immunofluorescence Negative Can Be Positive: Open AAV Data with... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog Post...Two new human lentiviral CRISPR library systems described in companion papers in Science in December were...Additionally, a concurrent Nature Biotechnology article describes the development of a mouse lentiviral CRISPR library... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post... optogenetic tool (CsChrimson) attached to a fluorescent indicator (tdTomato), as the effector. They also...expression in flies Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog Post...fascinating and fun look at her outreach work with fluorescent microbes. Check out her animation about the Hawaiian...repository (like these new Rainbow Bacterial Fluorescence Labeling Plasmids from the Barbier lab). Bright... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog Post...to bind fluorescent proteins derived from A. victoria the functionalized nanobodies described here can... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post... a feature inaccurately. The best example is fluorescent proteins, which often come in closely related...misidentification, we augment the database with new fluorescent protein variants. The future of DNA feature ...