We narrowed to 633 results for: gats

...partition is individually assessed for a positive or negative (digital) signal at the end of the reaction (Figure...contain any target DNA at the onset of PCR are negative, whereas those that did are positive. Since there...

...experience of our website, the different website navigation paths, the organization and completeness of our...all Addgenies involved). The new homepage and navigational elements help users who prefer to browse and...

... blog post, we covered how to use positive and negative selection in plasmid cloning.  But oftentimes...experiment, you’ll want the proper positive and negative controls for your PCR.  Find the details and learn...

...editing speed dictates which phage variants can propagate faster than the rate of dilution (passage from...off-target DNA and off-target RNA activity. To mitigate this drawback, the lab introduced an additional...

...mutations and truncations that can make vector propagation and sequencing a headache. How do I maintain ...integrity and prevent unwanted mutations?  During propagation, mutants rapidly replicate in a growth culture...

...extremely tricky to find: each of the factors can negatively impact both translation and fidelity of translation...possibilities for researchers, from selective protein ligation through introducing ncAAs with protein modifications...

... methylase adds a methyl group to the adenine of GATC stretches of DNA Dcm methylase adds a methyl group...and will therefore have methylated adenines in any GATC sequences found in the plasmid. When the PCR products...

...each one requires different elements for vector propagation. This post, along with a future companion post...yeast plasmid) allows for 50+ copies to stably propagate per cell. The copy number of these vectors can...

... use an input signal that both positively and negatively controls the output. In this case, plasmid copy...increases its expression. However, if you introduce negative regulation by using a promoter that is responsive...

...specific protein by combining it with the proximity ligation assay (PLA). The clever combination of FUNCAT ...endogenous protein complexes in situ by proximity ligation." Nature methods 3.12 (2006): 995-1000. PubMed...

...several years, it seems that the more polarized (negative) the action potential of a cell, the more differentiated...Brook T., et al. "Use of genetically encoded, light-gated ion translocators to control tumorigenesis." Oncotarget...

...large number of targets, and can require specific conjugates. And, of course, once you have them, you’ll need...antibodies, along with a higher likelihood of aggregation (Bates, 2019). They are small enough to be produced...

...problems. Why didn’t my PCR work? Why didn’t my ligation work? Why are there so many bands on my Western...process.  Step One: Identify the problem and gather information to help you understand it. Step Two...

...His work focuses on the enzyme hydropersulfide-propagating enzymes 3-mercaptopyruvate sulfurtransferase ...withdrawal to explore how these cells may encode the negative affect associated with drug withdrawal.  “Together...

...Later this week, postdocs and students will again gather in Boston - this time with the aim of finding solutions...programs? What would be the effects, positive and negative, of changing the workforce structure? Helping...

...spacer of each loxP site and the two strands are ligated, resulting in the reciprocal crossover event. This...sequence. Among these are loxN (GtATACcT), lox2272 (GgATACtT), and lox511 (GtATACAT). These variant lox sites...

...acquisition, storage, and utilization - and on self-propagation by reproduction. Both physiological processes...controlled by deliberate and compulsory actions instigated by the central nervous system signaling to peripheral...

...and selectable markers that allow students to propagate the plasmids in either E. coli (ori and ampR) ...and instructor resources is available at our Investigations in Molecular Cell Biology Website. Many thanks...

... culture supernatant and can be purified by centrifugation. Gag-Cas9 fusion protein The MLV Gag protein...which is both technically challenging and can negatively impact zygote viability. Nanoblades, however,...

...where and when certain genes are expressed to investigate their role in a specific behavior. This blog ...preceding the experiment. Handling can however negatively interfere with certain tests and is not always...