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Showing: 301 - 320 of 625 results

  1. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...kinase. This hybrid construct senses light, and an obligate chromophore allows the system to respond to various...photosensor senses various light inputs, and an obligate chromophore allows the system to respond. In far-red...

  2. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...luciferase-containing plasmid is most often used to investigate the effect of regulatory elements, such as promoters...Pathways Known cellular signaling pathways can be investigated in a high-throughput screen using luciferase...

  4. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...experience of our website, the different website navigation paths, the organization and completeness of our...all Addgenies involved). The new homepage and navigational elements help users who prefer to browse and...

  5. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...extremely tricky to find: each of the factors can negatively impact both translation and fidelity of translation...possibilities for researchers, from selective protein ligation through introducing ncAAs with protein modifications...

  6. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ... methylase adds a methyl group to the adenine of GATC stretches of DNA Dcm methylase adds a methyl group...and will therefore have methylated adenines in any GATC sequences found in the plasmid. When the PCR products...

  7. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...our SnapGene powered plasmid maps far easier to navigate. These practical and effective changes will give...restriction enzymes that are commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more...

  9. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...partition is individually assessed for a positive or negative (digital) signal at the end of the reaction (Figure...contain any target DNA at the onset of PCR are negative, whereas those that did are positive. Since there...

  10. A Deep Dive into BioBuilder

    Type
    Blog Post
    ... into modules that students and teachers can investigate together. Teachers decide which BioBuilder lessons...means to rapidly prototype DNA circuits and logic gates. This BioBuilder-Caltech collaboration has resulted...

  12. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ... blog post, we covered how to use positive and negative selection in plasmid cloning.  But oftentimes...experiment, you’ll want the proper positive and negative controls for your PCR.  Find the details and learn...

  13. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...each one requires different elements for vector propagation. This post, along with a future companion post...yeast plasmid) allows for 50+ copies to stably propagate per cell. The copy number of these vectors can...

  15. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...specific protein by combining it with the proximity ligation assay (PLA). The clever combination of FUNCAT ...endogenous protein complexes in situ by proximity ligation." Nature methods 3.12 (2006): 995-1000. PubMed...

  17. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...large number of targets, and can require specific conjugates. And, of course, once you have them, you’ll need...antibodies, along with a higher likelihood of aggregation (Bates, 2019). They are small enough to be produced...

  18. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...problems. Why didn’t my PCR work? Why didn’t my ligation work? Why are there so many bands on my Western...process.  Step One: Identify the problem and gather information to help you understand it. Step Two...

  20. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...Later this week, postdocs and students will again gather in Boston - this time with the aim of finding solutions...programs? What would be the effects, positive and negative, of changing the workforce structure? Helping...
Showing: 301 - 320 of 625 results