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We narrowed to 528 results for: genome

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Showing: 301 - 320 of 528 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector for mammalian genome editing Tet-pLKO-puro...Fusion Protein Function: Viral Vector Delivery, Genome Modification, mRNA Regulation, and More Selectable...can also be used for for viral vector delivery, genome modification, reporter assays, shRNA expression... efficiency, but do NOT integrate into the host genome Addgene has an extensive collection of plasmids...nondividing cells, integrate into host genome pLenti CMV GFP DEST - Lentiviral Gateway destination...Retroviral Easy and safe to use, integrate into host genome MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian...efficiency, but do NOT integrate into host genome pAdTrack-CMV - Shuttle vector for...

  2. Microbiology Resources

    Type
    Collection
    ...bacterial genome editing is not always straightforward. Read our CRISPR Methods for Bacterial Genome Engineering...using CRISPR in your lab, specific CRISPR tools for genome editing in bacteria, and more. Synthetic Biology...range of organisms. Find CRISPR tools for use in genome editing of bacteria. CRISPathBrick Multiplex Plasmid...opens in a new window) JGI Integrated Microbial Genomes and Microbiomes (IMG/M) (Link opens in a new window...NCYC) (Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window) Return...

  3. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...ion channels, despite having an otherwise normal genomic background, can even lead to the formation of whole...reprogrammed regenerating tissues without using genome engineering. Planarian worms cut into three segments... 2018) available at Addgene: MerMAID2 - A metagenomically discovered marine anion-conducting channelrhodopsin...

  4. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...or if you want to amplify sequences from a large genome organisms like mouse or human. There are several...amplifying a template, particularly from a large genome, you can recheck your primer design or optimize...

  5. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...resource from Jennifer Doudna’s lab at the Innovative Genomics Institute. What is CasPEDIA? CasPEDIA is an online...Beyond SpCas9 More resources from the Innovative Genomics Institute      CRISPRpedia IGI’s digital resources...

  6. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...researchers have adapted the components for use in genome manipulation. Cre recombinase, originally named... sequence does not occur naturally in any known genome other than P1 phage, and is long enough that there...

  7. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...Plasmid Collections Structural Genomics Consortium Plasmids Structural Genomics Consortium Plasmids You may...Plasmids from the Structural Genomics Consortium (SCG), which are primarily used for determining protein...like... David Waugh Lab plasmids The Structural Genomics Consortium is a not-for-profit organization that...

  8. Viral Production

    Type
    Collection
    ...sequencing of the Viral Genome Next-generation sequencing is performed on viral genomes isolated from the final...confirm the identity and integrity of the viral genome and the absence of unexpected DNA contaminants....pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] ...Electron Microscopy The ratio of empty to full (i.e., genome containing) AAV particles within representative...of a lentiviral vector, incubated for 72 h, and genomic DNA is extracted. Primers and probes targeting ...harvesting cells several days later, and isolating genomic DNA. This DNA is then used as a template to amplify...

  9. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...William RA, et al. "Serine recombinases as tools for genome engineering." Methods 53.4 (2011): 372-379. PubMed...Blog Learn How Piggy Bac Vectors Can Be Used for Genome Editing Learn about Michael Koeris' Journey from... Pages Check Out More Lu Lab Plasmids Find More Genome Engineering Tools  ...

  10. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...recombinase systems, how to use them to modify the genome or control gene expression, and find plasmids for...derive from fungi or bacteriophages and are powerful genome engineering tools used to precisely manipulate ... payload containing an attB target site into a genomic attP site. So far, their practical application ...site-specific recombinases can be used to modify the genome or control gene expression or skip ahead to browse... Vectors . Flp-IN: A single FRT site within the genome of a host cell line serves as the target location...stable integration of the gene of interest into the genome at the defined FRT site. Multiplexing: Orthogonal... may cause repetitive recombination leading to genomic instability and cell death. It is important to ...

  11. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...embryo derived-ES cells.They also conducted whole genome sequencing in selected control and CRISPR-Cas9 ...predicted off-target effects, they limited their whole genome sequencing to a small percentage of edited embryos...

  13. Important Considerations When Using AAVs

    Type
    Blog Post
    ...transcription. However, with scAAV the effective genome size usable is shortened (from ~4.8 kB [8] to ~...performance profiles and should be suitable for genome editing. These vectors can be used to improve targeted...adeno-associated virus serotypes: impact of larger genomes on infectivity and postentry steps." Journal of...

  14. AAV ddPCR Titration

    Type
    Protocol
    ...AAV titer range of 5E+12–5E+13 GC/mL, where GC is genome copies and generally represents the physical number...

  15. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...generation packaging systems further split the viral genome by removing the viral Rev gene from the packaging...This system improves safety by dividing the viral genome into additional plasmids thereby increasing the...

  16. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...this protein must undergo as it interrogates your genome. If you are interested in experiencing Cas9 up ...Addgene Blog Learn All about CRISPR Use CRISPR for Genome Visualization Tips for a First Time CRISPR User...

  20. Distribution to Industry

    Type
    Collection
    ...Plant Expression, Genome Editing Jim Haseloff 79 parts plasmids and vectors for genome engineering in the...Sinai Health System Plasmids from the Structural Genomics Consortium Solute Carrier Proteins (SLCs) from...vector libraries for CRISPR/Cas9-mediated saturation genome editing of human TP53 exon 5–8. Phagemid Synuclein...
Showing: 301 - 320 of 528 results