Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 539 results for: genome

Showing: 301 - 320 of 539 results

  1. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...-stranded intermediate into the single-stranded genome that's then packaged into AAV’s protein shell, ...: AAV titering by qPCR quantifies the number of genome-containing viral particles that are present in ...

  2. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...CNS). AAVs are popular tools because: 1) their genomes are easy to manipulate, 2) they have long-term ... it’s parent AAV.PHP.B: 1x1011 vs 1x1012 viral genomes (vg) per mouse. Figure 3 shows a gross comparison...

  3. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... sites, the phage integrates into the bacterial genome flanked by two new recombination sites (attL-left...fragments may be cloned: PCR fragments, cDNA or Genomic DNA and is available for all kind of organisms ...

  4. Editor's Choice, July 2016

    Type
    Blog Post
    ...with over 700 views) was an update on new CRISPR genome editing technology, Cpf1. Written by our Science...

  5. CRISPR Library Amplification

    Type
    Protocol
    ...RNAs targeting different genes in an organism's genome, for example. Amplification is usually necessary...

  7. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...purity testing, and sterility testing, plus viral genomic sequencing to confirm the identity of the packaged...

  10. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...alone, and this is expanding as we obtain more genomic data about plasmids (Shintani et al., 2015). Now...22153351 Shintani M, Sanchez ZK, Kimbara K (2015) Genomics of microbial plasmids: classification and identification...

  11. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...which can become costly and impractical for whole-genome (and often, whole-plasmid) sequencing.  Prior to...well as the ability to sequence an entire viral genome in one read. We also use Nanopore sequencing to...

  13. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...  IPTG-inducible expression of T7 RNAP from the genome; does not restrict methylated DNA fhuA2 lacZ:...expresses T7 RNA polymerase (RNAP) from the bacterial genome under control of the lac repressor, which is inducible...

  15. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems. Ahmed A. Gomaa, et...

  17. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...sequence, such as the Human papilloma virus (HPV) genome, via a crRNA. An ssDNA-fluorescently quenched (...Learn more about Cas12a (Cpf1) and its multiplex genome editing abilities Resources on Addgene.org Checkout...

  18. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...flanking regions comprised of upstream and downstream genomic sequences (Figure 2B).   Figure 2: A comparison... boxes. Highlighted blue regions show flanking genomic sequence. Created on BioRender.com. When it...

  19. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ... Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn about Science Communication using...
Showing: 301 - 320 of 539 results