We narrowed to 523 results for: genome

...Collections Structural Genomics Consortium Plasmids Browse All SGC Plasmids Structural Genomics Consortium Plasmids...Browse plasmids from the Structural Genomics Consortium (SGC) for 3D protein structure studies Plasmid...

...Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector for mammalian genome editing Tet-pLKO-puro...Fusion Protein Function: Viral Vector Delivery, Genome Modification, mRNA Regulation, and More Selectable...can also be used for for viral vector delivery, genome modification, reporter assays, shRNA expression... efficiency, but do NOT integrate into the host genome Addgene has an extensive collection of plasmids...nondividing cells, integrate into host genome pLenti CMV GFP DEST - Lentiviral Gateway destination...Retroviral Easy and safe to use, integrate into host genome MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian...efficiency, but do NOT integrate into host genome pAdTrack-CMV - Shuttle vector for...

...William RA, et al. "Serine recombinases as tools for genome engineering." Methods 53.4 (2011): 372-379. PubMed...Blog Learn How Piggy Bac Vectors Can Be Used for Genome Editing Learn about Michael Koeris' Journey from... Pages Check Out More Lu Lab Plasmids Find More Genome Engineering Tools  ...

...bacterial genome editing is not always straightforward. Read our CRISPR Methods for Bacterial Genome Engineering...using CRISPR in your lab, specific CRISPR tools for genome editing in bacteria, and more. Synthetic Biology...range of organisms. Find CRISPR tools for use in genome editing of bacteria. CRISPathBrick Multiplex Plasmid...opens in a new window) JGI Integrated Microbial Genomes and Microbiomes (IMG/M) (Link opens in a new window...Program (Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window) Return...

...Plasmid Collections Structural Genomics Consortium Plasmids Structural Genomics Consortium Plasmids You may...Plasmids from the Structural Genomics Consortium (SCG), which are primarily used for determining protein...like... David Waugh Lab plasmids The Structural Genomics Consortium is a not-for-profit organization that...

...sequencing of the Viral Genome Next-generation sequencing is performed on viral genomes isolated from the final...confirm the identity and integrity of the viral genome and the absence of unexpected DNA contaminants....pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] ...Electron Microscopy The ratio of empty to full (i.e., genome containing) AAV particles within representative...of a lentiviral vector, incubated for 72 h, and genomic DNA is extracted. Primers and probes targeting ...harvesting cells several days later, and isolating genomic DNA. This DNA is then used as a template to amplify...

...embryo derived-ES cells.They also conducted whole genome sequencing in selected control and CRISPR-Cas9 ...predicted off-target effects, they limited their whole genome sequencing to a small percentage of edited embryos...

...101 eBook to introduce students to this amazing genome enegineering technology Resources at Addgene.org...

...recombinase systems, how to use them to modify the genome or control gene expression, and find plasmids for...derive from fungi or bacteriophages and are powerful genome engineering tools used to precisely manipulate ... payload containing an attB target site into a genomic attP site. So far, their practical application ...site-specific recombinases can be used to modify the genome or control gene expression or skip ahead to browse... Vectors . Flp-IN: A single FRT site within the genome of a host cell line serves as the target location...stable integration of the gene of interest into the genome at the defined FRT site. Multiplexing: Orthogonal... may cause repetitive recombination leading to genomic instability and cell death. It is important to ...

...transcription. However, with scAAV the effective genome size usable is shortened (from ~4.8 kB [8] to ~...performance profiles and should be suitable for genome editing. These vectors can be used to improve targeted...adeno-associated virus serotypes: impact of larger genomes on infectivity and postentry steps." Journal of...

...this protein must undergo as it interrogates your genome. If you are interested in experiencing Cas9 up ...Addgene Blog Learn All about CRISPR Use CRISPR for Genome Visualization Tips for a First Time CRISPR User...

...generation packaging systems further split the viral genome by removing the viral Rev gene from the packaging...This system improves safety by dividing the viral genome into additional plasmids thereby increasing the...

...spreading through wild populations. Whereas most genomic changes impose a fitness cost and are eliminated...advance enabling RNA-guided gene drives is Cas9 genome editing. Finally, gene drives are an inherently...

... genome engineering...

... iPS cells? Confused by which system to use for genome editing? Know a professor with a great story to...

...AAV titer range of 5E+12–5E+13 GC/mL, where GC is genome copies and generally represents the physical number...

...endonuclease ability, to bind to genes without editing the genome (Qi et al., 2013). To target specific sequences...Gootenberg JS, Nishimasu H, Nureki O, Zhang F (2014) Genome-scale transcriptional activation by an engineered...

... 3, and of course the rapidly changing world of genome engineering via CRISPR.  Though the individual ... a thing if CRISPR hadn’t made manipulating the genome cheap and relatively easy? Have all of these things...

...be delivered to cells both transiently and for genomic integration. Lab-engineered viruses will package...package your genetic cargo in lieu of their own genomes and deposit this cargo upon host infection. Packaging...

...the vector may undergo genomic rearrangements during replication. To avoid genomic rearrangements, try amplifying...