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Showing: 301 - 320 of 878 results

  1. What's New in CRISPR - May 2019

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    Blog Post
    ...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...series, we’ll highlight a few of the new CRISPR plasmids available at Addgene. We will still periodically...Communications Biology Find the Cas9-PCV and PCV-Cas9 plasmids Selective curbing of unwanted RNA editing (SECURE...paper in mSphere Find the dCas9 and dCas9-Mxi1 plasmids If you have a new CRISPR tool you’ve recently...

  2. Plasmid Preps: Different Purity, Different Quantities, Different Uses

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    Blog Post
    ...Transformation introduces transformation grade plasmids into a bacterial cell. Transformation grade...that the preparation is pure from other nucleic acids (genomic DNA and RNA), proteins and chemical contaminants...Transfection grade DNA Figure 3: Need to introduce plasmids into mammalian cells? Use transfection grade DNA... Browse blog posts on plasmid cloning Read our Plasmids 101 blog posts Learn about the differences between...Addgene.org Read the molecular biology reference Find plasmids for your research Brush up on molecular biology...

  3. Seeing Red: Simple GFP Photoconversion

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    Blog Post
    ... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources... PubMed. McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL. A bright and photostable photoconvertible... Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach OM,...Resources Relevant Blog Posts Plasmids 101: Green Fluorescent Protein Which Fluorescent Protein Should I...

  4. Tag Your Favorite Yeast Genes with Ease

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    Blog Post
    ...Addgene distributes several ready-to-use, modular plasmids, combining fluorescent tags, epitope tags, protease...). Additionally, Dr. Formosa has deposited six plasmids with a multiple cloning site in place of the epitope...Jürg Bähler have deposited a large collection of plasmids for genome modification in yeast developed by ...Bähler et al.(2) describe a modular collection of plasmids for a wide variety of genome modifications in ...Longtine et al.(3) describe a complimentary set of plasmids for use in S. cerevisiae, with the additional ...

  5. Addgene is Expanding Our Viral Vector Service!

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    Blog Post
    ... more.  What will be available?  Over 3000 AAV plasmids in the Addgene repository will be available for...Packaged on Request service. A small subset of AAV plasmids will have technical or legal restrictions that...AAV1, AAV2, AAV5, AAV8, or AAV Retrograde (AAVrg) capsids.  Please be aware that this ordering process will...both our depositors and requestors.  Requesting Plasmids available for Packaged on Request consideration...its performance.   Titers We expect titers for plasmids packaged in AAV1, AAV5, AAV8, and AAV Retrograde...

  6. 2023 and 2024: Reflections and Looking Ahead

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    Blog Post
    ...18,359 viral items, and 166,247 plasmids. We welcomed 18,899 plasmids, deposited from 1,106 labs in 504...factor library, in lentiviral vectors. At 3500 plasmids, this represents the largest single deposit in...September, we released the fourth edition of our Plasmids 101 eBook. This updated version of one of our ...to announce that we are now able to ship rabies plasmids in our repository to international requestors....

  7. pSiM24: Simplifying Plant Genetic Engineering

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    Blog Post
    ...GreenGate Plasmids Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More Plasmids 101: Origin...transfer, studying the stability of modified Ti plasmids during plant infection, as well as plasmid yield...in two species: Agrobacterium and E. coli. Like plasmids used in other systems, binary vectors are cloned...Addgene Check out Addgene’s plant vectors! Find the plasmids from this paper at Addgene. Other relevant blog...

  8. Quick Guide to All Things Lentivirus

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    Blog Post
    ...Murine leukemia virus (MoMLV) and consisted of two plasmids: the packaging plasmid containing all the structural...the packaging signal Psi). Because the packaging plasmids lacked the psi signal, the genes encoding the ...generation of lentiviral vectors consisted of three plasmids encoding 1) the lentiviral vector genome which...stable expression of the transgene   Transfer plasmids The transfer plasmid is the vector you clone your... with your transfer plasmid and your packaging plasmids. For this, you can use the transfection reagent... and to get access to additional protocols and plasmids.  References 1. Miller, A. DUSTY, and C. A. R...

  9. A History of Genome Engineering in Popular Culture

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    Blog Post
    ... our CRISPR guide Find CRISPR plasmids for your research Browse plasmids for base editing References ...novel involves Dr. Moreau who creates human-animal hybrids. This book is published 30 years after the seminal...of the DNA get translated into the twenty amino acids. Once scientists established that DNA encoded all...cleavage specificity of a homing endonuclease. Nucleic Acids Research 30:3870–3879 . https://doi.org/10.1093/... (2003) Reprint: Molecular Structure of Nucleic Acids. Annals of Internal Medicine 138:581 . https://doi.org...

  10. Year of the Camelid: Antibody Style

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    Blog Post
    ...Assembly has declared 2024 the International Year of Camelids. The declaration is intended to raise awareness...populations around the world. Here at Addgene, we love camelids too, though not just for the reasons the UN describes... 12–15 kDa Ease of cloning and expression from plasmids, due to only needing one open reading frame Ability...generate and more widely adopted. So thank you to the camelids who inspired this versatile family of tools, the...Membrane Proteins More resources from addgene.org  Plasmids encoding nanobodies at Addgene Sybody Generation...

  11. Understanding GPCR Signaling Using TRUPATH

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    Blog Post
    ...effects.  Bryan Roth’s lab created a collection of plasmids called TRUPATH to interrogate 14 of the 16 non-visual...the TRUPATH system, cells are transfected with plasmids expressing the GPCR of interest, a Gα-Rluc8 plasmid...TRUPATH be used for? The Roth lab used the TRUPATH plasmids in a number of different experimental approaches...drugs to further understand their mechanism. These plasmids are available as a collection, facilitating screening...Addgene.org Browse the catalog of fluorescent protein plasmids Find biosensors for your experiments ...

  12. Sleeping Beauty Awakens for Genome Engineering

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    Blog Post
    ....12137. PubMed. Find the Plasmids at Addgene Kowarz Lab plasmids Izsvak Lab plasmids  ...The use of non-viral vectors, like transposons, avoids many, though not all, of these defenses. For some...for insertion in the correct orientation. These plasmids use a novel synthetic RPBSA promoter, which is...

  13. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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    Blog Post
    ...Addgene.org Find plasmids in Addgene's COVID-19 collection Find SARS-COV-2 pseudotype virus plasmids from Addgene...variants. “We created a series of spike expressing plasmids which encode spikes found on all of the different...response. The Balazs lab was quick to deposit their plasmids at Addgene to accelerate ongoing COVID-19 research...research. “I hope that by sharing the plasmids other scientists would be able to expand upon the work and...

  14. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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    Blog Post
    ...AraC and other promoter systems, head over to our Plasmids 101: Inducible Promoters article.) Modifying AraC... and shared with Addgene. You can find all the plasmids deposited from Di Ventura's lab, including the...nuclear export system (LEXY) here. Optogenetic plasmids from the Khammash lab can also be found here. ...Learn more about inducible promoters Visit the Plasmids topic overview page Resources on Addgene.org ...Molecular Biology Reference Download Addgene's Plasmids 101 eBook ...

  15. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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    Blog Post
    ...depositing your plasmids encoding antibodies, ScFvs, and nanobodies to Addgene! The plasmids will be made...working with the community to build a resource of plasmids encoding recombinant antibodies as well as other...through distributions of research materials, such as plasmids and viral vectors. However, it typically takes...Resources on Addgene.org Find recombinant antibody plasmids at Addgene ...

  16. Addgene Begins Distribution of Recombinant Antibodies

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    Blog Post
    ...resource: Recombinant Antibodies! In addition to plasmids and viral vectors, you’ll now be able to order...will have unique Research Resource Identifiers (RRIDs) for each antibody, making it easy for you to determine...other recombinant affinity reagents, share your plasmids via Addgene! Email [email protected] to learn...support, we have enabled scientists to easily share plasmids with each other. Together, we can transform the.../elife.43322 Bandrowski, AE, Martone, ME (2016) RRIDs: A Simple Step toward Improving Reproducibility ...

  17. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post
    ...homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and...editing efficiency. Smaller size At only 887 amino acids in length, NgAgo is shorter than SpCas9 (1368 aa.... Epub 2016 May 2. PubMed PMID: 27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan...24531762. PubMed Central PMCID: PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, ...DNA-guided nuclease that targets cognate DNA.” Nucleic Acids Res. 43(10) (2015):5120-9. PubMed PMID: 25925567...

  18. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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    Blog Post
    ...Looking for Optogenetics Plasmids? Plasmids for Optogenetics Research (Addgene) Plasmids from Ed Boyden's lab...conditions. Addgene: You've contributed various plasmids to Addgene over the years, many of them very popular... lab Sign up to receive email alerts when new plasmids from the Boyden lab become available. Visit Ed...

  19. Antibodies 101: Introduction to Antibodies

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    Blog Post
    ... are between 5-8 amino acids long, with a typical length of 5 or 6 amino acids (Cruse et al., 2004). Because...Antibodies may recognize a linearized sequence of amino acids, or only in their 3D conformation. Types of antibodies...sensitivity, for example by changing certain amino acids to improve its stability. Additionally, the choice...nanobodies, or synbodies, can be produced from plasmids, saving time and money. Nanobodies and synbodies...Addgene’s Antibody Plasmid Collection includes plasmids that express antibodies, nanobodies, and ScFvs...

  20. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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    Blog Post
    ...Protein Biosensors Browse Plasmids from the Tsien Lab Browse the Michael Davidson Collection ...fluorescent proteins everyday.  The late Michael Davidson and Roger Tsien played enormous roles in enabling...their legacy, we recently launched the Michael Davidson and Roger Tsien Commemorative Travel Awards. These... we’re proud to announce our first two Michael Davidson and Roger Tsien Commemorative Travel Awardees:...
Showing: 301 - 320 of 878 results