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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 318 results for: idua

Showing: 301 - 318 of 318 results

  1. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...of resistant cells start proliferating and the individual wells become confluent, expand into larger vessels...Generation of monoclonal cell lines from expansion of individual A549 cells stably expressing Cas9. A549 cells...for 9 days. Single cells were then plated in individual wells of a 96-well plate and left undisturbed...

  2. Protocol - How to Streak a Plate

    Type
    Protocol
    ...plasmid DNA from it, you will want to isolate an individual clonal population (single colony) of bacteria... proceed to Recovering Plasmid DNA or use the individual colonies for other experiments....

  3. Water Bath Protocol

    Type
    Protocol
    ...different sizes in the lab. Some water baths contain individual slots for heating up tubes while other water ...manual. If you’re filling up a water bath with individual slots for tubes, remember that when you add the...

  4. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...LB agar plates are frequently used to isolate individual (clonal) colonies of bacteria carrying a specific...copy number refers to the number of copies of an individual plasmid within a single bacterial cell. Large...

  5. Lentivirus Production

    Type
    Protocol
    ...post transfection in individual harvests or a combined harvest where all the individual harvests are pooled...

  6. Kit Free RNA Extraction

    Type
    Protocol
    ...overdrying, watch the pellet and carefully remove any residual ethanol wash and add RNase-free water or TE as...

  11. Pipetting Protocol

    Type
    Protocol
    ... then dispense to the second stop to expel any residual liquid in the tip. Before releasing the plunger...

  15. Pouring LB Agar Plates

    Type
    Protocol
    ...can hold 5-10 mL of agar and on which you can individually distinguish a maximum of ~100 colonies Pro-Tip...

  17. Western Blot

    Type
    Protocol
    ... Shake the gel gently between washes to remove residual water. Load the gel into the chamber of the SDS-PAGE...

  18. AAV Production in HEK293 Cells

    Type
    Protocol
    ...Benzonase is an endonuclease that will degrade any residual DNA carried over from the packaging process. Incubate...
Showing: 301 - 318 of 318 results