We narrowed to 315 results for: idua

...LB agar plates are frequently used to isolate individual (clonal) colonies of bacteria carrying a specific...copy number refers to the number of copies of an individual plasmid within a single bacterial cell (Figure...

...post transfection in individual harvests or a combined harvest where all the individual harvests are pooled...

...overdrying, watch the pellet and carefully remove any residual ethanol wash and add RNase-free water or TE as...

...Many of the precautions are meant to protect individuals from potential accidents such as spills. BSL-...

... or restriction enzyme -based cloning to test individual clones before use of more expensive forms of ...

...components except for the DNA. Aliquot your DNA into individual tubes and then add the appropriate amount of ...

...take a fresh tube, thaw it one time and aliquot individual tubes of 5, 10 or 20μL for storage at -20°C. ...

... then dispense to the second stop to expel any residual liquid in the tip. Before releasing the plunger...

.... For details on how to streak a plate to get individual colonies and to generate liquid bacterial cultures...

...Finished Plasmid Finally, you will need to pick individual bacterial colonies and check them for successful...

...Multiply the volume of each reagent by the number of individual PCRs you wish to perform and add 10% extra to...

...can hold 5-10 mL of agar and on which you can individually distinguish a maximum of ~100 colonies Pro-Tip...

...Finished Plasmid Finally, you will need to pick individual bacterial colonies and check them for successful...

... Shake the gel gently between washes to remove residual water. Load the gel into the chamber of the SDS-PAGE...

...Benzonase is an endonuclease that will degrade any residual DNA carried over from the packaging process. Incubate...