...drive high levels of expression of certain viral components; according the manufacturer these changes result...number of harvests will all impact the titer. Importantly, optimized conditions for one transfer plasmid...producing lentivirus, it is time to titer. One important consideration when titering a lentivirus is the...

...publication.   3’UTRome The 3’UTRs of mRNA are important for post-transcriptional regulation in C. elegans...system allows scientists to both spatially and temporally knock-down proteins of interest.  Plasmids for...intersectional strategy using a split intein for refined spatiotemporal transgene control in Caenorhabditis elegans...

...Calcium is arguably one of the most important metal ions in biology (sodium and potassium are up there...blood clotting, and as we all know, a structural component of bones and teeth. It is an enzyme co-factor,..., and an allosteric modulator. Because of its importance, scientists have developed calcium sensors to...

...pair to another base pair at a specific site. 2. Temporal, spatial, or conditional control of CRISPR activity... way to make those genome edits. But, just as important is a way to flip the switch and turn off CRISPR...The ability to turn off CRISPR activity is an important step towards CRISPR-based therapies and for more...

...homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. Lu et al. Plant J.....  Controls  When designing experiments, it’s important to include proper controls to assess the behavior...target proteins, especially if the termini are important for their interaction. You can read our previous...

...well as the introduction of the cancer-fighting component, CAR. Clinical trials are underway to explore ...effectiveness, and delivery. Patient safety is important for allogenic therapies to prevent a graft vs....vs. host response. Off-target effects are also important safety considerations for all CRISPR therapies...

..., and North America. Diagnostics will play an important role in helping to monitor and slow this spread...American-lineages). Strain discrimination is especially important in the current outbreak because of the neurological...of Zika Virus Using Programmable Biomolecular Components." Cell (2016). PubMed PMID: 27160350. Find Plasmids...

...that infect bacteria), many of which are doing important work in our microbiomes. Given that viruses are...undergo DNA rearrangements. Depending on the composition of the viral vector and the repeat sequences ...ensuring viral production is to perform PCR against components of the viral vector using the harvested viral...

...was the outdoor market where one can shop for components of traditional Chinese medicine. There were hundreds...field. Nobel Laureates and the ever expanding importance of RNA The second keynote lecture was presented...in his lab. RNA is a structural and functional component of the ribosome complex and Dr. Steitz quipped...

...-aggressives, who are usually antagonistic and impolite, Complainers, who spend most of their time moaning...solutions that will meet both of your needs. 6) Put important agreements in writing One of the most frequent...from your supervisor. Whenever possible, discuss important issues in person or over the telephone instead...

... purposes. We’re here to help with that! Four important factors to consider for any experiment are: ...Efficiency For cloning in particular, another important consideration is: The number of inserts ......, like Gibson or SLIC, since one can pool the components and define their final order through sequence...

...combs. To make genetic crosses properly, it is important to isolate females from males while they are still... after hatching into their adult phase. It is important to clear out the parent crosses from the container...feature when setting up genetic crosses. More importantly, this knowledge will stop you from embarking ...

...publishing is important to you, make sure that your project will not involve proprietary compounds or information...communicate your science to others and advocate for the importance of your work. In my experience in industry, you...

...setting up assembly reactions in which a single component was purposefully omitted. Since any assembly is... no blue colonies were obtained if any single component was omitted. Screening for successful Golden ...The future of assembly DNA assembly methods are important tools for many areas of science, and researchers...

...carefully engineered to support reliable functional composition. In other words, these parts can be used over...YouTube primer on BIL gates.) Addgene: Why is it so important to you that these parts be made freely available...language is put into practice? Endy: I think it's important to understand that these platforms, or pieces ...

...presentation because, in my opinion, it was more important to communicate the applications of CRISPR over...use it? This last section was arguably the most important, and it was a little worrisome for me since bioethics...biology, are moving so quickly that it’s basically impossible for textbooks to keep up. Even the most ambitious...

...your samples, the relationships between cellular components, and the accessibility of your target epitope...work by dehydrating and precipitating cellular components. This method of fixation is good for epitopes...away your target (for example, some cytoskeletal components) and you want to fix and permeabilize in one ...

...by ddPCR begins with diluting the virus. It is important to note that the dynamic range of the ddPCR is...PCR plate. Maintaining a high droplet count is important for calculating the concentration of your sample...separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150 base pairs...

...analyzable transgenic in hand, so it is pretty important to get this process right the first time. I’ll...to transform when they begin flowering. It is important to perform the transformation not long after flowering...s time to select for transformants. It’s very important to select multiple T1 plants from each transformation...

...gut, and other organs to the CNS, are also an important target for gene delivery, especially for the study...3293995. Additional Resources on the Addgene Blog Important considerations when using AAVs Learn more about...research. Visit Our AAV Guide Page for info on AAV components, common uses of AAV, and much more! Find ready-to-use...