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We narrowed to 658 results for: otos

Showing: 301 - 320 of 658 results

  1. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...encoded calcium indicators. Cold Spring Harbor Protocols. 2012 Jun 1;2012(6):pdb-top069609. Palmer AE, ...genetically targetable fluorescent indicators. Nature protocols. 2006 Aug;1(3):1057-65. Resources on Addgene.org...

  2. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...ligand co-developed by Bryan Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise...with the IPD of a cation channel, such as the serotonin 3 receptor, will promote depolarization in neurons...

  3. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...anti-mouse antibody. Others recognize specific isotopes within that species, like an anti-mouse IgG antibody...sample. There are species-on-species staining protocols that use antibody fragments to block the endogenous...

  4. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...a group discussion on the best strategies and protocols for different experiments.  In this blog post...and resources page Browse our molecular biology protocols Read our molecular biology reference ...

  6. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...reported the development of a rapid design and prototyping pipeline that can take pathogen sequence information...critical needs and to further develop our rapid prototyping and sensor assembly pipeline for future emerging...

  7. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... a new window) . Protocols The Allen Institute for Cell Science has several protocol videos available ...Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute for Cell Science has produced...

  8. AAV Packaged on Request

    Type
    Collection
    ... your AAV experiments, remember to share your protocols and results through Addgene’s Data Hub . By contributing...globe and shared our well-developed and trusted protocols and peer-reviewed techniques (Link opens in a ...preps. Data Hub Access Addgene’s Data Hub for protocols and results shared by other scientists using AAV...

  9. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...that is complementary to the target site, or protospacer, in the genome. Native Cas9 has two catalytic...which cleaves one strand of DNA upon binding the protospacer. The resulting double strand break (DSB) stimulates...

  11. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...Titration of baculovirus can vary depending on the protocol used. Plaque assays are standard but can take ...More resources on Addgene.org Addgene's virus protocols Viral Plasmids and Resources References Shaw, ...

  12. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ..., & Zoltowski, B. D. (2021). Cryptochromes: Photochemical and structural insight into magnetoreception...Matsuoka, R., Kimura, S., Miura, T., Ikoma, T., & Kusamoto, T. (2023) Single-Molecule Magnetoluminescence...

  14. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ... incubated together to form the RNP. Detailed protocols outlining these steps have been made publicly ...Polyethylene Glycol (PEG) mediated transfection of protoplasts and biolistic bombardment of immature embryos...

  15. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...across systems and species. The cost? Zeocin is genotoxic when not 100% inhibited, meaning it will induce...Addgene.org Addgene’s Basic Molecular Biology Protocols Over-Agar Antibiotic Plating Plasmids 101 eBook...

  16. An Introduction to Adenovirus

    Type
    Blog Post
    ...adenovirus type 5 (hAdV-C5) can be considered the prototype, although many viruses infecting humans (from ...oxfordjournals.aje.a113563. PMID: 6301263. Servellita, V., Sotomayor Gonzalez, A., Lamson, D. M., Foresythe, A., Huh...

  17. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ... absorbance of both is then measured on a spectrophotometer and concentration versus absorbance is plotted... time, etc., which can vary even when the same protocol is being followed.  Finally, standard curves must...

  19. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...that increased the fluorescence intensity and photostability of GFP. This also shifted its major excitation...Known Function S65T Increased fluorescence, photostability, and a shift of the major excitation peak to...

  20. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...increased particle stability; however, the protein is cytotoxic precluding the long-term expression of lentiviral...Overview of Lentiviral Packaging Plasmids Lentiviral Protocols and Resources More Lentiviral FAQs Answered by...
Showing: 301 - 320 of 658 results