We narrowed to 309 results for: pET

...bacterial strain and you will not need to obtain more competent cells and retransform. Bacteria on an LB agar ... the tube and use a sterile loop, toothpick or pipette tip to scrape some of the frozen bacteria off of...

...stab or glycerol stock . Using a sterile loop, pipette tip or toothpick, touch the bacteria growing within...plate, to create streak #2. Using a third sterile pipette tip, toothpick, or sterilized loop, drag through...

...restriction digest. Equipment Electrophoresis chamber Pipetman Pipet tips Reagents Liquid DNA aliquot of your plasmid...

...autoclave. Only mechanical pipetting should be done in the laboratory. Don’t mouth pipette! There may be some ...

... formulation buffer and sample and make sure to pipet back and forth a few times to mix the iodixanol ...buffer. Use a P1000 to the bottom of the filter and pipette up/down and wash off the walls of the filter to...

...needed because several microliters can be lost in pipetting. For example, if you want to load 1.0 μg in 10μL...

...annealed oligos). Transform 2-3μL into your favorite competent bacteria and plate. Be sure to pick multiple colonies...

... LB in a 2 L Erlenmeyer flask. Using a sterile pipette tip or toothpick, select a single colony from your...

...Interpretation Uncut vector - Checks viability of competent cells and verifies the antibiotic resistance of...