We narrowed to 304 results for: pET

1. Protocol - How to Purify DNA from an Agarose Gel

   Type

   Protocol

   ...needed because several microliters can be lost in pipetting. For example, if you want to load 1.0 μg in 10μL...

2. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

   Type

   Protocol

   ...annealed oligos). Transform 2-3μL into your favorite competent bacteria and plate. Be sure to pick multiple colonies...

3. Protocol - How to Inoculate a Bacterial Culture

   Type

   Protocol

   ... LB in a 2 L Erlenmeyer flask. Using a sterile pipette tip or toothpick, select a single colony from your...

4. Protocol - How to Ligate Plasmid DNA

   Type

   Protocol

   ...Interpretation Uncut vector - Checks viability of competent cells and verifies the antibiotic resistance of...