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TypeBlog Post...This post was contributed by guest blogger Gairik Sachdeva. Healthcare consulting is a fast-paced field...their knowledge to a variety of problems. In this post, I’ll share what I’ve learned as a healthcare consultant...and occasionally being faced with “shifting goal posts” as a part of a case, consulting can sometimes be...
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Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post... View Other CRISPR Blog Posts Additional Resources Zhang lab Cpf1 blog post on Benchling ...experimentally confirmed. To prevent new editing post-HDR, repair templates should remove the PAM sequence... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post... Resources Read other posts about life at Addgene!Read more of our careers posts! ...successful event at your company. The point of this post isn’t to make you all jealous that you don’t work...Spread word of your plans by email, gong, evite, posters, megaphone, or your company’s intranet. Don’t forget... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post...We've discussed promoters before so in this blog post we’ll cover basics of multicistronic vectors: why... plasmids. We've combined our Plasmids 101 blog posts and additional resources into one downloadable PDF... 2008 May 11.) PubMed. Read more Plasmids 101 posts or browse Addgene's Plasmid Guide for more molecular... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post...are transcribed from the RNAP III promoters. This post features promoters for general RNAP II and RNAP ...constructs and we will discuss these in a future post on viral vector parts. Promoter specificity Aside...Resources on the Addgene Blog Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about Reporter... -
A History of Genome Engineering in Popular Culture
TypeBlog Post...This post was contributed by Kartik Lakshmi Rallapalli, a graduate student at the University of California...preference than the ZFNs. Genetic Engineering Era: Post CRISPR CRISPR The strategy of using a protein to...the Genome Editing Trading Card seen in this blog post?Download high resolution versions here. Many thanks...Additional resources on the Addgene blog Find more blog posts about CRISPR Learn more about the history of CRISPR... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post...This post was contributed by guest blogger Eleanor Wang, a Research Specialist in Patrick Hsu's lab at...continue my scientific endeavors. I hope that this post can provide some perspective and advice to any undergraduate... you. Be proactive Rather than applying only to posted job offerings, the best research experiences typically... offer application fee waivers. One of the blog posts that helped me quite early on was Christine Liu’...graduate and/or undergraduate classes Postdoctoral researcher (postdoc) Getting more scientific and professional...or 9-to-5 job. I had to ask to find out what a “postdoc” is, and that “PI” stood for principal investigator...admin duties Staff scientist: similar role to a postdoc but more permanent Visiting scholar/student: visiting... -
Plasmids 101 eBook 4th Edition
TypeBlog Post...sections, including our popular "Dimers and Multimers" post. It's also been re-organized and updated with a ... -
Beginner's Guide to Viral Vectors
TypeBlog Post...virus experiment. Subscribe to Viral Vector Blog Posts from Addgene Need more information on viral vectors... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog Post...graduate student in the Roth lab, for editing the blog post. References Olsen RHJ, DiBerto JF, English JG, Glaudin...blog Browse all of our fluorescent protein blog posts Find other fluorescent biosensors Resources on ... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog Post...which contains the viral genome. In today’s blog post, we’ll focus on the AAV transfer plasmid and take...and combinations out there not discussed in this post, you’ve got the knowledge to be able to design a...WPRE The Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) forms a tertiary... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog Post...) and subject age at time of injection. How long post-injection did you wait until performing the experiment...process of designing your experiment, read our blog post on important considerations when using AAVs. Experimental... -
New and Upcoming Viral Vectors - December 2019
TypeBlog Post...For more details on this technology, see our blog post Overview of AAV Encoded Calcium Sensors). Plasmid...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral ... -
Intro to Our Summer SciComm Series!
TypeBlog Post...for our first post, I thought it best to define language you’ll see throughout the posts and introduce...field, often through manuscripts, seminars, or posters. It is important to note that it is the actual ... -
The Strength of Story Telling
TypeBlog Post... This post was contributed by guest blogger Jack Leeming Editor of Naturejobs, part of Nature magazine...Resources on the Addgene Blog Check out our blog posts on science communication Find interviews scientists... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog Post...using CRISPR/Cas9 RNPs? Check out this post Check Out Other Blog Posts on CRISPR Additional Resources on ... -
Tips for Improving Your Next Manuscript
TypeBlog Post...This post was contributed by guest blogger Sean Stacey who recently attended both online and on site ...publications. My classmates consisted of graduate students, post-grads, and early career scientists. Over the course... -
Using Addgene's New Viral Service for Your Research
TypeBlog Post...vector educational resources as well, with both posts on the blog and pages on the Addgene website. blog.addgene.org...virus. You can find all of our viral vector blog posts here. If you have virus expertise you’d like to ... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post...springs My husband, Paul Blainey, at that time a post doc in the Quake Lab, volunteered to do the sampling... other Cas proteins Read all of our CRISPR blog posts Read about GeoCas9, another thermostable Cas9 Resources...was wonderful to learn that one project led by postdoc Dr. Stephanie Schmidt not only produced partially... -
FastCloning: A Newer, Simpler Cloning Technique
TypeBlog Post...This post was contributed by guest blogger Meghan Monroy, a graduate student in Protein Science at the...on Your Plasmid Background with our Plasmids 101 Posts Resources on Addgene.org Find Empty Vectors for...