We narrowed to 526 results for: sequence

...selection cassette with 5′ and 3′ flanking sequences matching the sequences upstream and downstream of the open...

...work in a very narrow window proximal to the PAM sequence, some base editing systems create a wide spectrum...

...However, to allow for more room for regulatory sequences while still fitting Cas9 and its gRNAs into one... systems: St1Cas9 requires a very specific PAM sequence that limits the number of targetable loci, and...enrichment on streptavidin and next-generation sequencing). Using this sensitive method, they found that...

...edits (St. Martin et al., 2018). To circumvent sequencing and the need for a DSB in their previous reporter...need for single cell isolation and downstream sequencing (Standage-Beier et al. 2019). TREE is a real-...codon of AUG immediately downstream of a kozak sequence. The Dow lab hypothesized that if they created...

...targeting system could be used to routinely deliver sequences of interest to cells with efficiencies of 1-40%...CRISPR is remarkably effective for introducing non-sequence specific frameshift mutations through aberrant...template. It is this application - the creation of sequence-specific knockins - for which the AAV approach...currently focuses on determining the mechanisms and consequences of cohesin gene inactivation in human cancer...

... however, Cas9 allows you to easily change the sequence targeted by the activator by simply providing ... consist of plasmids that over-express coding sequences from a given cell type or organism, have been ...several popular CRISPR-based tools for altering DNA sequence or regulating gene expression.     References ...

...dendrites under the control of internal ribosome entry sequence in a viral vector Hillel Adesnik 111066-AAV1 pAAV-hSyn-dLight1.1...Cre-dependent expression; soma localization targeting sequence GENIE Project 172908-AAV1 pGP-AAV-syn-FLEX-Ace2N...Cre-dependent expression; soma localization targeting sequence GENIE Project 174007-AAV9 pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST...and ChrimsonR are separated by cleavable peptide sequence P2A. Mark Histed 179459-AAV1 pAAV-EF1a-DIO-JEDI...

...pipeline and accelerated the processing of our sequencing data.”  Recently, Seqera published a case study...

...vectors and plasmids with repeating sequences. Repeating sequences within a plasmid can undergo intramolecular...

...host machinery to make additional copies. ori sequences are generally high in As and Ts. Why, you ask?...that affect plasmid copy number  Although the sequence and regulation of the ori dramatically affect ...

...often used to quantify DNA for next generation sequencing. Unlike absorbance-based methods, fluorescence-based...method is often used preceding next-generation sequencing or microarray studies and is not used as much...

...discussion, we sent it for sequencing. The gene was successfully sequenced, confirming that we had made...

...protein – you first need the DNA sequence that encodes it. That DNA sequence then needs to be incorporated...

.... For example, Dave Grawoig is using nanopore sequencing at BosLab to perform proof-of-concept experiments... to collect various soil samples, extract DNA, sequence it, and analyze the data to characterize the microbiomes...

.... Different antibodies bind different protein sequences called epitopes and scientists can use these to...different targets, the scientists fused them to sequences called complementarity determining regions (CDRs...

...to target an ssRNA of interest, for example, a sequence specific to a virus or pathogen. Once Cas13 recognizes...recognizes and binds to the programmed sequence, it can promiscuously cleave surrounding ssRNA molecules...SHERLOCK. Cas12a is targeted to a specific DNA sequence, such as the Human papilloma virus (HPV) genome...

...amino acid sequence required for BirA recognition and biotinylation. This 13 amino acid sequence is termed...Biotin can be added to either terminus of a DNA sequence during a Polymerase Chain Reaction (PCR) by using...

... Both the plasmid (blue, backbone) and the DNA sequence of interest (green, insert) are cut with restriction...in the direction you want - usually by Sanger sequencing or further restriction digests. Of course there...appropriately sized insert, you should send it for sanger sequencing using primers that will allow you to read over...

... downstream methods, such as qPCR or next-gen sequencing. ChIP is an incredibly useful application for...suitable for isolated and purified DNA, such as sequencing or qPCR. Yay!  Where’s the challenge in that?...

...eukaryote (S. cerevisiae) to have its genome fully sequenced There are several human disease models established...Genome manipulation K. phaffii’s genome has been sequenced for some time, clearing the greatest hurdle for...