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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 538 results for: sequence

Showing: 301 - 320 of 538 results

  1. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...behavior, many many images of brain regions, or sequences of microbial DNA they all seem to require some...biological tools. In addition to the researcher using sequencing technologies to study microbiome interactions...

  2. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ... of DNA to create a synthetically designed DNA sequence. There are multiple methods of DNA assembly available...experimental and primer design based on fragment sequences and the polymerase used in the amplification. ...

  3. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...eukaryote (S. cerevisiae) to have its genome fully sequenced There are several human disease models established...Genome manipulation K. phaffii’s genome has been sequenced for some time, clearing the greatest hurdle for...

  4. Antibodies 101: ChIP

    Type
    Blog Post
    ... downstream methods, such as qPCR or next-gen sequencing. ChIP is an incredibly useful application for...suitable for isolated and purified DNA, such as sequencing or qPCR. Yay!  Where’s the challenge in that?...

  5. Mammalian RNAi Tools

    Type
    Collection
    ...double-stranded RNA (dsRNA) that matches the mRNA target sequence. The dsRNA can be delivered as shRNA (short hairpin...remaining single-stranded siRNA then binds the target sequence and RISC cleaves and degrades the mRNA. RISC includes...

  7. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...scaffold and cloning sites for inserting the guide sequence (pSpCas9(BB)). The 2A-GFP allows for screening... plasmid for cloning and expressing new shRNA sequences. For more information, see the Addgene’s pLKO.1...

  8. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...are necessary for recognition and cleavage. This sequence flexibility indicates that gRNAs could be incorporated...tRNA-gRNA and tRNA-gRNA-tRNA, with short 5’ spacer sequences preceding gRNAs, to test for gRNA cleavage. gRNAs...

  10. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...libraries will require access to next generation sequencing technology. These libraries are lentiviral ... including a tool for getting optimized guide sequences for activating any human gene, go to http://sam.genome-engineering.org...enabling systematic investigation of the cellular consequences of repressing or inducing individual transcripts...

  11. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...PEDAR strategy to delete and replace long genomic sequences. On the ninth day of CRISPR, Addgene gave to me...

  13. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...flanked by unique restriction sites so you can swap sequences effortlessly.  We built these plasmids for flexibility... flanking an RNA payload to insert the payload sequence into the genome. The team established that they...elements allowed for scarless insertion — no UTR sequence added to the genome (Figure 4). Figure...

  14. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory...

  15. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ... vector-free cells Genome integration Need to sequence cells to verify excision didn’t introduce mutations...integration Multiple transfections required Need to sequence to verify there’s no genomic integration Replicating...gene(s) of interest flanked by terminal repeat sequences, which the PB transposase recognizes. Following...can occur at the transposon insertion site, so sequence-verification is required. Non-integrating...

  16. The 12 Days of CRISPR

    Type
    Blog Post
    ...editing for cleavage-free genomic knock-in of long sequences🎵On the 2nd day of CRISPR, Addgene gave to me:...

  17. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    ...diseases. Spontaneous mutations that change protein sequence affect arginine (Arg) amino acids more frequently...facilitated by CRISPR reagents and other specific gene sequences available from Addgene. Dr. McMurray hopes that...metabolic diseases with severe and life-threatening consequences. They result from defects in different steps...

  19. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...gene may have errors introduced to the nucleotide sequence during the DNA repair process. The addition of...recombinase catalyzes the recombination of DNA sequences located between two loxP sites. The loxP sites...
Showing: 301 - 320 of 538 results