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We narrowed to 734 results for: son

Showing: 301 - 320 of 734 results

  1. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...the statistical independence assumption of the Poisson process. This modification also eases the interpretation...orders.  Our model factors the likelihood in our Poisson process into two components: 𝜃x(t) and 𝜓z(t)....contains an intractable integral of the product of a Poisson and multivariate log-normal likelihoods. Effects...

  2. An Introduction to Adenovirus

    Type
    Blog Post
    ...genes that apparently contribute to virulence (Tollefson et al., 1996) – cell death has been reported when...6301263. Servellita, V., Sotomayor Gonzalez, A., Lamson, D. M., Foresythe, A., Huh, H. J., Bazinet, A. ...10.1182/BLOOD-2007-02-062117. PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, ...

  3. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...qPCR runs before a new one is needed. For these reasons, we are in the process of transitioning our titering...for the detection of low copy numbers. For this reason, it can be used in the detection of rare sequences...PMID: 24328707. PubMed Central PMCID: PMC3991984. Song, Neng, et al. "Detection of circulating Mycobacterium...

  4. Tips for arabidopsis transformation

    Type
    Blog Post
    ...Arabidopsis is a fantastic model organism for many reasons, not the least of which is ease of transformation...a protocol for antibiotic selection, check out Harrison et al., 2006. Tips: Don’t plate seeds too densely... 24032037. PubMed Central PMCID: PMC3759027. 2 Harrison, S. J. et al. (2006) ‘A rapid and robust method...

  5. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...vertebrate embryogenesis. To illustrate some of the reasons that zebrafish have been embraced as a model organism...examine the consequences of manipulating genes. One reason for the success of zebrafish as a model organism... 2016). One common and robust strategy uses a transposon called Tol2 to randomly integrate foreign DNA...

  6. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ (2018) NmeCas9 is an intrinsically high-fidelity.... Kim E, Koo T, Park SW, Kim D, Kim K, Cho H-Y, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim ...Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered...

  7. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...mammalian cell lines. However, in those same comparisons, the AbuGoot lab reported no detectable effect...and can be easily programmed for new genes. In comparison with other large insert techniques like HITI ... Rachel Leeson...

  8. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...screening! This blog was written by Addgenie Hannah Dotson.  Resources and references References Genome-...protocols.  References McDade JR, Waxmonsky NC, Swanson LE, Fan M. Practical Considerations for Using Pooled...  Hannah Dotson...

  9. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...meeting who felt isolated at a conference for any reason was a Singleton. Figure 1: The Binning Singletons...Gorski, Mark O. Martin, Maureen Berg, Michael D. L. Johnson, Tiffany Lowe-Power, Eric Griffis, Janet Iwasa,...Dunivin, Jen Greenwich, Susanna Harris, Heather Hendrickson, Jonathan Jacobs, Mo Kaze, Christina Kellogg,...

  10. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ... can arise, and has arisen, for many different reasons. Exposure to antibacterial drugs provides a selection...households by observing proper cleanliness and personal hygiene standards. Handwashing is an essential...spread of antibiotic resistant bacteria from one person to the next. For more information and tips on preventing...

  11. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...lack of documentation and high turn-over of lab personnel. If you are unsure of the background of the lines... such as serum, other cell lines, or infected personnel and can persist undetected; unlike infections ..., Kassem H.S., Pepper S.D., Hey Y., Ward T.H., Margison G.P. “Mycoplasma infection significantly alters...

  12. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...samples during cryo-EM grid plunge freezing By Rachel Leeson Cryo-EM samples can be damaged at the air-water...  Anzalone, A. V., Gao, X. D., Podracky, C. J., Nelson, A. T., Koblan, L. W., Raguram, A., Levy, J. M....Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., … & Weissman, J. S. (2021). Genome-wide...

  13. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ... a fluorophore (Figure 1).   Figure 1: Comparison between how direct immunofluorescence and indirect... will focus on this technique here. One of the reasons that indirect IF is more common is that secondary...primary, resulting in signal amplification. Another reason is cost effectiveness. Generating and validating...

  14. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...different projects, you might not be able to make any reasonable progress on any one of them. That’s why, as I...recently started using Trello to manage larger, multi-person projects. Day-to-day, flexible to-do lists. Because...Bored and Brilliant” for learning more fantastic reasons to remove yourself from distractions every once...

  15. Evolution of Lab Techniques

    Type
    Blog Post
    ...be quantified by the strength of the signal in comparison to a control sample. Southern blotting applications... sequencing of many samples at once for later comparison. These next-generation sequencing techniques ...Sanger, Frederick, Steven Nicklen, and Alan R. Coulson. "DNA sequencing with chain-terminating inhibitors...

  16. Finding Your Perfect Job After University

    Type
    Blog Post
    ...being mentally challenged. For these and other reasons, I started  looking for other career options after...government loans to study a MSc, so I had to take out a personal bank loan. I saved money from my role in Industry...or another country of the Commonwealth. Another reason why I chose Australia was because it is an English...

  17. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...cross country boundaries for both professional and personal development, is essential to excelling in the ...adapt their mentoring styles to the mentee’s personality and professional needs at any time. In its Pilot...advisor. For their own part, mentors receive immense personal gratification as they witness their student’s ...

  18. Important Considerations When Using AAVs

    Type
    Blog Post
    ... were suitable for our needs for the following reasons: Safety: Since AAVs are nonpathogenic, we are...Ready-to-Use Retrograde AAV Preps at Addgene 2) Comparison of genetically engineered receptors (i.e. chemogenetics...applications, we may also be able to use these comparisons to enhance our understanding of receptor physiology...

  19. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...better plan your transductions and use a range of reasonable MOIs. 2. Create a monoclonal Cas9-expression ... gRNA plasmids on our website. There are a few reasons to target different regions of the gene with multiple...testament to how far genome engineering has come. For comparison, in 2007 it took about 3 months to knockout a...

  20. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...that contains sequence complementary to the dsRNA (Wilson and Doudna, 2013). RNAi consists of two non-exclusive..., Deans RM, Li A, Bassik MC (2016) Systematic comparison of CRISPR/Cas9 and RNAi screens for essential...:dev186452 . https://doi.org/10.1242/dev.186452 Wilson RC, Doudna JA (2013) Molecular Mechanisms of RNA...
Showing: 301 - 320 of 734 results