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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 938 results for: Kin

Showing: 161 - 170 of 938 results

  1. Pipetting Protocol

    Type
    Protocol
    ...amounts of liquid (think: 0.1 µL to 1 mL). When working in a laboratory, properly dispensing liquid ensures...plunger. Remove the entire pipette from the container making sure not to touch the sides of the container with...

  2. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...syringe and a blunt edge 18 ga Hamilton needle, taking care to avoid bubbles (Figure 1). 8 mL of 15% iodixanol...facing the 40% step. Collect up to 5 mL per tube, taking care to avoid collecting the proteinaceous material...

  3. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...Wear a lab coat, eye protection and gloves when working with this chemical. Note: If you add EtBr to your...the DNA. Note: When using UV light, protect your skin by wearing safety goggles or a face shield, gloves...

  4. AAV ddPCR Titration

    Type
    Protocol
    ...the risk of contaminating reagents we recommend making small aliquots of master mixes, primers, and probes...wells. Cover the cartridge with the DG8 gasket, making sure that it is secure. Transfer the cartridge ...

  5. CRISPR Library Amplification

    Type
    Protocol
    ... 30-37 ℃, 225 rpm for 1 hour. After the 1 hour shaking period, pool and gently mix the four tubes. Perform...transformation efficiency! Ensure that no arcing is taking place during electroporation. Arcing would manifest...

  6. Lentivirus ddPCR Titration

    Type
    Protocol
    ...wells. Cover the cartridge with the DG8 gasket, making sure that it is secure. Transfer the cartridge ...the risk of contaminating reagents we recommend making small aliquots of master mixes, and primers/probe...

  7. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...business of iPSC clone picking, a 100-fold decrease is the difference between picking 100 versus 10,000 clones...efficiency.   But what’s better: PAM-blocking or guide RNA blocking mutations? This depends entirely on...efficiency of guide RNA blocking mutations. So if the locus does not allow blocking the PAM sequence (e.g...intended mutation while taking advantage of the improved efficiency provided by blocking mutations. Essentially... incorporation of the blocking mutation. Despite the fact that both the blocking mutation and our intended... same CRISPR/Cas-blocking mutation but not the intended mutation. Here, the blocking-only templates will...used CRISPR/Cas9 extensively for the purpose of making sequence-specific changes in human induced pluripotent...

  8. Finding Your Perfect Job After University

    Type
    Blog Post
    ...experience working in a developing tropical country. This is why I set up a placement working in a rural...work-life balance compared to working in research. My previous experience working in Industry was really valuable...sustainable. After working at this company for several years, I was ready for a change.  Working abroad: Research...new science graduates and the pro’s and con’s of working in different sectors. Luckily, I have worked for... decide for themselves.  Here is my experience looking for the perfect job. Summer work experience in... explain why I changed jobs. I highly recommend taking on a similar position in your field of interest...and problem solving. I believe that is something lacking during degree courses; they never really prepare...

  9. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...insufficient blocking) or reduced signal (too much blocking.) If you’re having issues with your blocking, first...it, try making fresh running buffer. Old or improperly made running buffers can cause all kinds of odd ..., degraded or improperly mixed blocking buffer, or insufficient rocking during incubation steps.  Many...pattern in your background, try asking more experienced labmates or looking it up online. You’ll find many...ResearchGate’s western blot forum.  Blocking Don’t skimp on the blocking time! Fill the time with smaller...  Troubleshooting Blocking reduces the amount of background in your blot, so blocking issues can either...you can try either longer blocking times or higher concentrations of blocking buffer. If you see a low ...

  10. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...room, so it is a good idea to start thinking about public speaking early and often. Two of my jobs have...supposed to be done. They will start looking at their phones. Speaking longer just makes you seem unprepared...choosing controls, or mixing clear solutions—public speaking is skill that any scientist can learn.  Any time...how to get it, especially if introverted ), and networking (how to get people to remember you). What better...Watch other people give great talks We are not talking about lab meeting here. Lifeless presentation of... Series. Watch how these speakers talk without looking at their slides or notes. Listen to their stories...and policy content. Watch Joanne give her "Not" Networking 101 Talk 2. Tailor every talk to the occasion...
Showing: 161 - 170 of 938 results