We narrowed to 650 results for: CHI;

1. Plasmids 101: TOPO Cloning

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   Blog Post

   ...mediated by vaccinia virus DNA topoisomerase I in Escherichia coli is sequence specific."  Proc Natl Acad Sci...

2. In Vivo Biotinylation of Bacterial Fusion Proteins

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   ... bacteria refuse to grow. Eventual success was achieved by inserting BirA into a pMal plasmid (NEB) thus...

3. The Strength of Story Telling

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   ...you don’t like it yourself. Be proud of your achievements and accept and acknowledge your failures. Foster...

4. Meet the New Addgene Board Members!

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   ...profound.” Frank Stegmeier, PhD Frank Stegmeier is the Chief Scientific Officer at KSQ Therapeutics and is an...

5. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

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   ...scientists with complete plasmid sequences. After launching our viral service in 2016, we adapted this platform...

6. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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   ... community resource. Instead of painstakingly searching for experimental details in research articles,...

7. New and Upcoming Viral Vectors - December 2019

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   ...While the various AAV serotypes can be used to achieve different patterns of expression, researchers often...

8. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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   ...Addgene also performs independent research? Since launching the viral service in 2016 Addgene’s research team...

9. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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   ... database. Through a series of simulations and machine learning, they created a classifier that predicts...

10. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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    ...offer numerous advantages, including more secure archiving, unambiguous molecular definition, and potential...

11. Safe Port in a Storm...How Addgene is Weathering the Pandemic

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    ...on track to succeed at the same time. I began approaching science funding organizations to request support...

12. Summer SciComm: Science Storytelling

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    ...way through a series of obstacles in pursuit of achieving knowledge. Or, as we in the storytelling business...

13. RUBY-Red Siliques

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    ...the segregation of the CRISPR construct could be achieved much easier than by PCR or a fluorescent microscope...

14. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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    ...salt-stress induced responses in Root System Architecture of Arabidopsis HapMap population in Amsterdam...

15. Hot Plasmids - January 2023

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    Blog Post

    ...promoter) drives expression of the tumor-seeking CAR (chimeric antigen receptor), whereas a ubiquitous promoter...

16. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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    ... Lins B, Mietzsch M, Heilbronn R, Van Vliet K, Chipman P, Agbandje-McKenna M, Cleaver BD, Clément N, Byrne...

17. Addgene Depositors Get More Citations

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    ... lends weight to the argument that Addgene is achieving its goal of making plasmid-based science more ...

18. With Our New Viral Service, We're Taking CRISPR Further!

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    ...infection and at least one screening-scale experiment achieving the gRNA representation recommended in the publications...

19. Learning Technical Skills at a Lab Central Workshop

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    ...Illumina sequencing and took a class at NEB’s new teaching space at Lab Central to learn all about it. LabCentral...

20. Reagent Repositories Are Speeding up Science During the Pandemic

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    ...CC-Y, Tsang JO-L, Huang X, Chai Y, Yang D, Hou Y, Chik KK-H, Zhang X, Fung AY-F, Tsoi H-W, Cai J-P, Chan...

21. Scientists Map the SARS-CoV-2-Human Interaction Network

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    ...millions, if not billions, of dollars, but by approaching it from a new direction, we hope to do it much...

22. What Good Citizenship Can Do for Reproducibility in Science

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    ...Cell Stem Cell.  She was the founding Editor-in-Chief of Cell Stem Cell, a role she held for 10 years,...

23. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    ...dynamic range and high brightness. This reporter achieves approximately 100-fold increase in fluorescence...

24. Early Career Researcher Toolbox: Free Tools for Science Writing

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    ...for writing scientific manuscripts: literature searching, reading, and organizing Tips for writing a scientific...

25. Networking Follow-up: I Met a Person. Now What?

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    ... was only one other person in my class who had children. When I had my baby, we became closer. She supported...

26. Celebrate National Book Month with Addgene

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    ...book club before, but I had a few reasons for launching this one. On a purely selfish level, I wanted ...

27. New and Upcoming Viral Vectors - September 2019

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    ...between peak fluorescence and resting fluorescence (achieved through lowering sensor resting fluorescence, ...

28. Pushing the Limits of DNA Assembly

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    ...of sgRNA libraries, with maximum complexity, is achieved with synthetic oligos with a randomized region...

29. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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    ...formation of a healthy microbiome. In order to achieve this goal, Fatima first needed an efficient and...

30. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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    ... found in DNA. It then recruits transcription machinery to the site to induce gene expression. Thus, genes...