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Showing: 481 - 510 of 810 results

  1. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...plasmids in the library are differently sized, smaller plasmids may be amplified preferentially, skewing...Brunello library is shown in Figure 2. You can use a small PCR product because the plasmids in the library ...

  2. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...yourself to be as productive as you would be in a normal situation. For some other resources on working...Science, or Resources to Engage Kids at Home. It’s normal and okay to feel overwhelmed right now, but hopefully...

  3. Hot Plasmids - January 2023

    Type
    Blog Post
    ...alternatives - RNAi and Cas13. CRISPR-Csm doesn’t degrade proximal RNAs nor does it have high levels of off-target...changes of non-target genes and RNA-seq of a nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from...

  5. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...examining relationships between microbes and insects, mammals, and plants, among others. I particularly enjoyed...doesn’t love a book series that features a young female protagonist, some clever magic, and a satisfying...

  6. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...recently released our serotype testing AAV, which are small (20 ul) samples of EGFP-expressing AAV packaged ...resting fluorescence and can be used for imaging small neuronal processes (dendrites and axons). jGCaMP7c...

  7. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...generations of iGluSnFR had limitations: they were not optimal for experiments requiring high signal to noise ...fusion to the PDGFR transmembrane domain in the mammalian expression pMinDisplay vector. GPI contains a ...

  8. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important elements...enzymes cut at known sites within the ITR. However, small deletions and point mutations will go undetected...

  9. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ... Assembly method from New England Biolabs® has minimal upfront requirements and allows you to expediently...generation, green chemistry and understanding the minimal genome. Many of these techniques require more complex...

  10. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...PAM-distal side of the break and 91bp on the PAM-proximal side, consistently supported higher frequencies...attained without any of the error-prone repair that normally accompanies Cas9 cutting. We still don’t know ...

  11. Components of CRISPR/Cas9

    Type
    Blog Post
    ...or closely related Cpf1) to cut the genome and a small RNA (gRNA) to guide this nuclease to a user-defined...used for CRISPR-mediated genome editing, these two small RNAs have been condensed into one RNA sequence known...

  13. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... attain cell-specific expression in transgenic animals, where cre expression is restricted to a particular... which can then be exclusively activated by the small molecule clozapine N-oxide (CNO) (Krashes et al....

  14. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...reverse tetracycline-controlled transactivator) is normally inactive and cannot bind the tetracycline response...Similarly, the various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique promoters...

  15. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...H2B (histone)-GFP reporter into the genome of mammalian cells.  Figure 1: The SRIRACCHA reporter...create GEmCherry2. The Denham lab made several other small changes creating 2 other versions but these did ...

  16. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ... replication, repairing mismatched basepairs or small indels that occur during DNA synthesis, and protomoting...preceded by GA or followed by TC) would be cut as normal. If not taken into account, this blocked site could...

  19. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...resulting in two independent Fab fragments with small tails of the hinge region. Size: ~55 kDa.  Fab -...handful of advantages over full antibodies. Their smaller size allows them to get into samples more efficiently...

  20. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...problem is, when it happens to other people, it’s normal and quickly forgotten, and when it happens to you...noises were just last week!  Nerves It’s completely normal to be anxious, nervous, and/or apprehensive. I ...

  22. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ... possibly because in vitro conditions did not optimally support growth of diverse species. These results...Carlotta, et al. "Metagenomic engineering of the mammalian gut microbiome in situ." Nature methods 16.2 (...

  23. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...becoming available to the community. This is no small task, however, at a repository that consistently...supplements), suggested sequencing primers, or mammalian selection markers. You don’t need a fancy database...

  25. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...specific, targeted DNA modifications in transgenic animals, embryonic stem cells, and/or tissue-specific cell...6276558. 2. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage ...

  27. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...persistent immunological reconstitution with a normally functioning T cell repertoire (1). In this example...icosahedral, non-enveloped virus that carries a small, approximately 4.7 kb single stranded genome. AAV...

  28. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...environmental plasmids, are all around us! A plasmid is a small piece of DNA that can replicate independently from... genetic parasites. Cryptic plasmids tend to be small and poorly studied, but some researchers have suggested...

  29. Hot Plasmids February 2024

    Type
    Blog Post
    ... it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA...editing frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines...

  30. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...transitioned between different lab groups without having a formal interview process. Industry interviews, however...structured. I met with several individual and/or small groups, usually starting with the hiring manager...
Showing: 481 - 510 of 810 results