We narrowed to 877 results for: HA

... tyrosine generating a GFP variant (GFPY66) that has a shifted emission spectra. Thus a successful base...luciferase. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable...

...high for both plasmids, so each plasmid “thinks” it has a higher copy number than it actually does, and neither...large, low copy virulence plasmids. This strategy has been successful in displacing, or “curing” the virulence...

...lab to split my cells later. Eating with friends has a somewhat immediate deadline - even the best of ...can be quite difficult. In my experience, planning has 3 components: A mechanism for blocking off time ...

...instances where it does. 1) When the backbone vector has multiple BbvCI sites. If these sites have opposite...example like this. 2) When your gene of interest has a BbvCI site(s). If so, the nicking mutagenesis protocol...

...properly into the right and left hemisphere. Addgene has several plasmids that contain SHH, you can find them...in nonmuscle systems in the fly. Spaghetti squash has been studied in many contexts within the fly including...

...FLP efficient The first version of FLP discovered has a temperature optimum of 30 °C and is therefore inefficient...evolution led to the identification of FLPe which has a temperature optimum of 37 °C (Buchholz, Angrand...

... virulence (Tollefson et al., 1996) – cell death has been reported when using vectors, and immune inactivation...the infection to sometimes result in cell death, has led to interest in using adenoviral vectors for vaccine...

...eBook, How to Get a Faculty Position. This edition has substantial expansions on the chalk talks and Zoom...I also incorporated example documents. This part has generated a lot of positive feedback. Many of these...

...applicable in DSB-induced genome engineering, it has biological importance for the repair of breaks that...directed repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made...

...mycoplasma contamination. Mycoplasma contamination has been shown to arise from a variety of sources such...line for mycoplasma and discard it if the culture has become overly confluent or too old. Ensuring that...

...bacteria and extremophiles for biotechnology and has been directly involved in supervising many successful...

...soon as the homepage opens. You’ll notice it now has two options: “Search Repository” and “Deposit Materials...

... evenly with a sterile spreader until all liquid has been absorbed by the agar. This usually takes 1-2...

...payment during checkout. Placing your order online has some benefits. All of the information that is entered...

...cornerstone for The Addictive Brain. Our community has also requested live question and answer sessions ...

...Addgenies what their favorite career tip is, or what has helped them the most. Take a look below! In the lab...

... Currently in its 16th year, Science in the News has two chief goals. First, SITN works to bridge the ...

...available from several vendors. The precipitation method has an unfortunate side effect of messing up the reaction...

...less than 5 cents per transformation. This protocol has worked well for us for multiple E. coli strains, ...

...is important to determine how much phosphorserine has actually been incorporated into the full-length protein...protein. Up to 80% p-Ser incorporation into E17TAG GFP has been achieved with the Rinehart system (6); however...

...tough job! Kamel Khalili’s lab at Temple University has demonstrated two potential strategies for CRISPR-...approach might be more successful. “Shock and kill” has the advantage of killing cells infected with HIV-...

... through the impact of COVID-19. I think Addgene has done a really good job at figuring out how to operate...

...bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages like reduced off-target activity and decreased...

...Good bye 2020! What a year it has been for all of us. As we eagerly await 2021, we've looked back on ...

... Addgene Blog Learn How the Clon Tracer Library Has Been Used in Cancer Lineage Tracing Read How CRISPR...

...another field maybe, and publish Join a lab that has multiple collaborations, even with Industry labs ...

...or authors’ own web sites. 9)      The year 2013 has also been the year of the massive open online course...

...sealer and press the ‘Seal’ button. After the plate has been sealed, proceed to thermocycling. Thermal Cycling...

...characterization of over 1,000 mutations, and the group has deposited several hundred plasmids from this study...

...small number of people, deserves our attention and has huge potential for the development of therapeutics...

...Platform at the Broad. His mom always told him that he has the best words and fingers of a completely normal...

...outside the team, and bring information back. No team has all the answers – reinventing the wheel is inefficient...

...FRCaMP (itself based on mApple, which means it likely has the blue-light photoswitching issue). Although similar...CRISPR/Cas9 experiments By Ashley Waldron CRISPR/Cas9 has revolutionized biotechnology — partly because of ...

...reproducible” does not necessarily mean that someone has replicated it precisely. You can find a great discussion...easier to understand. The need for great stories has, however, made it difficult to publish so-called ...

...cost-effective method that our lab (the Szymanski lab, which has recently moved from the University of Alberta in ...Edmonton, Canada, to the University of Georgia, USA) has developed that allows for a phage genome to be probed...

...in state machine technology? Biological research has produced a massive amount of information regarding...two-input gene regulation programs. The software has two components - a database of pre-compiled register...

...different species/isotypes and that each secondary has a different color fluorophore that you can image ...abundant in your samples (with a primary antibody that has the same species and isotype as the primary antibody...

...would not end up in a life sciences career but life has a way of making unexpected decisions. As someone ...

...to treat a 25 y old woman with CF whose infection has failed all #antibiotics. We need lytic non-lysogenic...

...PAM motif, averaging 19 targets per gene. The lab has previously used the library with SpdCas9 to perform...