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We narrowed to 986 results for: IGH@;

Showing: 641 - 680 of 986 results

  1. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ... LEGO and science: The potential is endless One night, while opening a LEGO set with my husband, I thought...that resembles a real laboratory. It only took one night to build the first model, but because we are [email protected]. Who knows, your story might just get “legofied.” Many thanks to our guest blogger...

  2. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...Chemistry symposium and a career night. The society also visits high schools to promote chemistry degrees...scientific meetings for students to attend. It also hosts high level academic debates for its members to become...

  3. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...arrive. Getting off the plane in Bozeman Sunday night, it was easy to spot meeting participants. They ...the ones holding poster tubes (or as our baffled flight attendant called them, "long, skinny things") and...thinking I was an engineer. The talks we heard Monday night represented engineering first and foremost, from...

  4. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ... allow his team to work mostly at home. Having a high degree of freedom and diversity built into their...new things, diversity of responsibilities, which might include training or teaching others, research, and...sometimes taken to an extreme by scientists) Are highly mobile and can move to a new workplace if opportunities...

  5. 10 Ways to Share Your Science!

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    Blog Post
    ...telling it another way. You’ll know you are on the right track if they start asking you questions. Remember...Escape from the Ivory Tower.”  9)   If you think you might want to get serious about science writing, look ...News. Ask your institutional news office if there might be opportunities to blog or write for them. 10)  ...

  6. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...bacteriostatic reagents can kill bacteria when used at high concentrations (and vice versa). Looking around ...Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media hotter than about 55oC...55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline is used. ...

  7. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...Baradaran says.  Baradaran’s path to science began in high school. “I had a great chemistry teacher in 10th...medicines can have on human health. As a teenager in high school, I knew that this is what I wanted to do,...ClonTracer Barcoding Library, a library that “enables the high-resolution tracking of more than 1 million cancer...

  8. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...and it is under copyright, you will need to obtain written approval from the copyright owner and will ...treatment conditions like drugs (Chemogenetics) or light (Optogenetics) to activate receptors? If you’re ...

  9. Fluorescent Biosensors for Measuring Autophagic Flux

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    Blog Post
    ...Keima accumulates in autolysosomes, this ratio is high. When untargeted, Keima can be used to measure bulk...autophagy-related microtubule-associated proteins 1A/1B light chain 3B, or LC3. LC3 is a commonly used marker ...oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy Resources on Addgene.org...

  11. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ...forward. Here are a few out-of-the-box ideas that might jump start your learning. 1. Create a peer mentoring...what’s a scientist to do with no live audience in sight? You could do a talk virtually for your peer mentoring...university or use your current contacts to ask who they might suggest you speak with. See this post that’s all...

  12. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...their secondary structure, palindromic nature, and high GC content. This instability can cause partial loss...through ITRs due to their secondary structure and high GC content, but some companies now offer Sanger ...example, CAG is a strong promoter that drives very high, ubiquitous expression, while the human synapsin...

  14. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...makes them want to know more. This could be by outright stating the big-picture problem, by asking your...science storytelling. You can introduce your results right at the beginning of your communication, use conflict...storytelling options, and they all can be effective in the right circumstances.   However, in the midst of all these...

  15. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...the ‘bells and whistles’ – including microscale light emitting diodes, thermal sensors, and microfluidic...channels. These features enable the fibers to emit light for optogenetic purposes, measure temperature, study...construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused to mCherry to the...

  16. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ...Delivery The RNP delivery method consistently leads to high on-target editing and low off-target editing rates...SpCas9 The closer your edit is to the PAM site, the higher the efficiency of HDR repair. Check out this post...post to see if an engineered Cas9 variant might work for your experiment. PITChing MMEJ as an Alternative...

  17. What Do I Do Now? Academic v. Non-Academic Career Decisions

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    Blog Post
    ...surprisingly more rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually... <- Can move between the two (helps to know the right people) -> How can you make a decision about ...interests and passions is another great way to shed light on what paths to pursue. Spend some time really ...

  18. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...’d like to show our gratitude on the blog by highlighting some of the great posts written by Addgene depositors...to activate neurons and neuronal pathways using light and chemicals respectively. Didem Goz Aytruk from...

  20. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...determine genome-wide translational frequency. Although highly useful, this technique requires extensive sample... nucleus to bind cognate untranslated mRNAs. The high specificity and resolution of this system are achieved...SunTag Catch up on Your GFP Background Choose the Brightest Fluorescent Protein Resources on Addgene.org ...

  22. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...but thought we could learn a little bit more about high throughput DNA prepping by trying out NEB’s new ...generating a wide range of insert sizes and also produced high yields of quality libraries from small amounts of...summarize my experience in my first blog post! I highly recommend doing the same if you ever go to a workshop...

  23. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...viral samples. Read on to learn how aqueous samples might be affected by dry ice, and specifically how dry... removing the caps, incubating the tubes in an upright position for 30 seconds, and then recapping the...to prevent any pH change, as we still observed a slight change in pH (from 7.4 to 6.8). It’s possible that...

  24. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...as virus? “Like all things in science, getting a high titer Cas9 lentivirus prep takes a bit of time and...lentivirus, we are providing lentiviral preps containing highly requested gRNAs including those targeting EGFP,...library has retained gRNA representation, produce high titer lentivirus from the library, ensure gRNA representation...

  25. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...Director of Biology, Lianna Swanson, suggested that I might be able to attend the 10 Years of iPSCs Symposium...or tertiary, or non-existant). On the very first night of the iPSC symposium, a panel of thought leaders...Resources on the Addgene Blog Learn about Efforts to Fight Rare disease with iPSCs Read About Addgene's Outreach...

  28. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...narrow down search results by adding more terms.  Weighting We determined the relative importance of different...given resource, and designed the search engine to weight search results differently according to where a...matches of a name in the depositor field will rank higher than matches of the same name in the publication...

  30. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...their former interns to fill entry-level positions right out of college. Prior to March 2020, internships... a nationwide database of  STEAM internships for high school, college, and graduate students. In order...interview, inspect your technology, background, and lighting for a smooth performance. Most importantly, make...

  31. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...complicated? Well, while the techniques are straightforward, the systems – the cells growing and producing...And on top of that you need to worry about getting high enough expression of your protein of interest. If...by using Addgene’s Blue Flame award to identify highly requested GCE plasmids. But they’d also love scientist...

  32. Viral Vectors 101: The AAV Retrograde Serotype

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    Blog Post
    ...variant that efficiently infects neurons, directs high-level transgene expression, and has retrograde functionality...genetic tool delivered by the AAV.   AAVrg requires high titers for efficient retrograde transport. For recombinase-dependent... at the injection site (Fischer, KB. 2019). The higher the titer, the more likely you are to see spurious...

  33. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...this context, but also specifically study it. They might be researching enhancer elements that control the...research describe the production of plasmids that might be useful for your work. The links below direct ...techniques, the posts below may steer you in the right direction! CAPTURE-ing Chromatin Interactions: Using...

  34. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...of mutant target (as low as 20 pg or 0.02%) in a high background of wild type targets. Comparatively, ...M. A Digital PCR-Based Method for Efficient and Highly Specific Screening of Genome Edited Cells. PLoS...PubMed Central PMCID: PMC4735761. 6. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas...

  35. Plasmids 101: Blue-white Screening

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    Blog Post
    ...). As shown on the representative plate to the right, colonies with an insert-containing plasmid have... plates at 4C for few hours after the initial overnight incubation increases pigment precipitation, enhances...colonies. Take care in making your plates: X-gal is light and temperature sensitive and needs to be added ...

  36. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...interested in the same things you are on a deep level. Right away you have something in common. Namely, the scientific... field will know about grants and projects that might help your work Tenure can depend on your national...some of my best studies were designed over late night poker games at Gordon Conferences). You don’t want...

  37. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ... read an 11-page Cell paper - and sometimes, it might actually take me less time to read the book.  Intuitively...science communication, but the more you use, the higher cognitive load you are asking your audience to ...like the professor who once decided that to teach high school students PCR, they first had to understand...

  38. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...zygotes at 18 hr post fertilization. They observed higher targeting efficiency than in iPSCs, but with the...scientists proceed with caution and with proper oversight from regulatory and ethics committees comprised... Check out Our New CRISPR Topic Page Read about High Fidelity CRISPR Variants Target Multiple Genes at...

  39. Antibodies 101: Conjugation

    Type
    Blog Post
    ...antibodies that produce detectable signals like light or color. In this post, we’ll go over some of the...chemiluminescent reaction. Chemiluminescence, which is light produced by a chemical reaction, can be detected...Whatever the conjugate, the molecules must somehow be tightly linked to your antibody. This chemical coupling...

  40. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...working with an endogenous locus. The most straightforward way to turn off expression permanently is to...- this section is for you! Many of the tools highlighted in this section can be used to turn expression...
Showing: 641 - 680 of 986 results