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We narrowed to 1,006 results for: RING

Showing: 641 - 680 of 1006 results

  1. The Challenges of Cell Culture

    Type
    Blog Post
    ...majority of cell line problems come from people sharing cells: the black market of cell biology. Engaging...cell culture, microbiology, or virology without acquiring materials from this bank. I am often asked, “What...cells to be used and STR profiling to be conducted during projects to ensure the results are reliable. With...

  2. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...successful inhibition of hippocampal neurons. Initial monitoring Second, this may sound rudimentary, but before...exciting—and unanticipated—road of discovery. Monitoring behavioral output The appropriate behavioral ...science writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org...

  3. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...The first time I heard about FRET during a journal club, my guitarist brain automatically thought about... recently and is the most rigorous method for measuring FRET. FLIM measures the fluorescence decay time...MW. Fluorescent proteins for FRET microscopy: monitoring protein interactions in living cells. Bioessays...

  4. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...  Communication (or lack thereof): Information sharing gets more challenging the bigger an organization... can help, and if and when you find yourself transferring this skill to another job, it will be important...conflicts is not inevitable. When you know you are bringing this asset to your job, you can become a valuable...

  5. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...Immunofluorescence (IF), is an immunoassay that brings to light the cellular world. The technique allows...poorly in a given context, let your community know! Sharing your experience, positive or negative, will help...Immunofluorescence Staining. In: Methods in Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/...

  6. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...sequence in the way that they like, the design and engineering of fusion proteins is relatively straightforward...assessing oligomerization in cells. In addition to measuring stickiness, several researchers have also collected..., Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in single cells." PloS one 13.3...

  7. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...need to test for this in your system if you’re measuring expression over a period of weeks (in vitro) or...to turn on, or turn off, gene expression by administering an inducing agent like doxycycline (Favre et...cells you want to deliver to, and how you’ll be measuring your experimental output. You’ll also want to ...

  8. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...Hippocampus labeled with anti-Kv2.1 (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels...expect the target to be. You can test this by preparing a sample without the primary antibody, or with...Yong (Ed.), Biobanking (Vol. 1897, pp. 299–311). Springer New York. https://doi.org/10.1007/978-1-4939-8935...

  10. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...make lentiviral vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent...lines but reach their limits when it comes to transferring DNA into primary cells or non-dividing cells...virus. Even though this system was efficient at transferring the gene of interest to the appropriate cells...

  11. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    ...pipetting! We’ll release a new tip every two weeks during the school year. Our first quick tip, “Your Bench...  Want your quick tips even quicker? We’ll be sharing our favorite parts of every quick tip video on ...

  12. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...collection of memorabilia, even made an appearance during co-founder and CSO Melina Fan's words of welcome...you all so much for an amazing twenty years of sharing science!       ...

  13. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    ... What a fun video of Mario, dressed as Santa, delivering gifts to the Mushroom Kingdom. Kravitz Lab 2019...snowflakes, ornaments, lights, and took a group photo wearing holiday sweaters and Santa hats. There was even...

  14. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    ...Bacterial Expression Systems. Check out our Genome Engineering Overview and our CRISPR guide to find out how...how CRISPR is being used for bacterial genome engineering. To make it easier for you to find plasmids ...

  15. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...plasmid-based technologies. Commentary about scientific sharing and the open-access movement. At Addgene, we believe...the conversation! Do you have a question about culturing iPS cells? Confused by which system to use for...

  17. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ...standardized way. Since Addgene encourages the public sharing of aggregated data only, information on requesting...you find the dashboard useful when analyzing and sharing your own depositor metrics!  About the Author Joachim...

  18. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...packaged on request, you’ll see a banner below the ordering table. Click on it, select your desired serotype...find this service useful in your research. Happy vectoring! ...

  19. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...vectors repository since March.  Addgene is now offering an AAV2 CAG-driven tTA expresser from the Gradinaru...PdCO, deposited by the Yizhar lab. Addgene is now offering AAV1 and AAV5 preparations of several variants...

  21. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    ...direct impact on human health and look forward to hearing from her in the future. Future awardees We will...to learn more about future awardees and their inspiring research. Additional Resources on the Addgene...

  22. Editor's Choice, July 2016

    Type
    Blog Post
    ...Intern (and soon to be Addgene Scientist!) Mary Gearing, this post focuses on new data from the Kim and...concrete context. I recommend this post to anyone wondering if and how they can apply FRET in their own research...

  23. The Addgene Antibody Data Hub

    Type
    Blog Post
    ... your research, you've probably found yourself staring at a browser full of tabs, each open to a different... a report with a DOI for citation. If you’re considering requesting an Addgene antibody, the Data Hub ...

  24. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    ...extremely proud to be able to support scientists by bringing you all the plasmids you need. We're focused on... on our mission – documenting your plasmids, storing them, providing quality control and customer support...

  25. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...infected in the United States while caring for a patient, despite wearing protective gear, and now comes news...

  27. What's New in CRISPR - May 2019

    Type
    Blog Post
    .... coli genome-wide CRISPR inhibition Covalent tethering of DNA template to Cas9 SECURE base editors Nme2Cas9...genome-wide CRISPR inhibition library Covalent tethering of DNA repair template to Cas9 Homology-directed...

  28. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...few of these trainees are sufficiently prepared during their 6-12+ (!) years of training to get jobs. ...designing career exploration programs or peer mentoring groups. But for now, you can click here to register...

  30. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...ultrasound. In his research he applies naturally occurring bacterial “molecular buoyancy devices” (also known... Post on CRISPR Methods for Bacterial Genome Engineering Learn about Science Communication using Twitter...

  31. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ... service. We've had 11 blog posts on COVID-19 featuring plasmid tools from depositors who quickly shared...19 pandemic, we saw the need to expand reagent sharing even broader and began working with tech transfer...

  33. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...2016 was an exciting year for genome engineering research. A variety of new tools came out including ...here. References 1.Ran, F. Ann, et al. "Genome engineering using the CRISPR-Cas9 system." Nature protocols...

  35. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    .... “What is different is the plasmids you use to bring this into your target organism. We have designed...,” Lohmann said. “We strongly believe the free sharing of material is absolutely essential for scientific...

  36. Let There Be LITE Plasmids

    Type
    Blog Post
    ...transcription-activator-like effectors (TALEs) as anchors for genome engineering applications was still very new. Now, Konermann...flexible their LITE system could be by applying it to altering gene expression via epigenetics with the addition...

  37. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...viral service in late 2016 and requests are still pouring in! While our lentiviral service is going strong...which cells are successfully infected by an AAV by altering your injection site and choosing a specific serotype...

  38. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...Farewell 2019, and hello 2020! As we're gearing up to start a new decade, we’re also looking back at ...strategies for generating GFP fusion proteins. From considering protein size and shape, linker sequences, and...

  39. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ...broken down below for specific topics discussed during the interview. Happy listening! Listen to the interview... Sit'N Listen - In their podcast, the SITN team brings in scientists from a variety of fields as well ...
Showing: 641 - 680 of 1006 results