We narrowed to 903 results for: nes

... shift requires deliberate preparation and a willingness to embrace new responsibilities. After my second...between technical and managerial roles, and I've witnessed several colleagues do this while changing industries...

...the different populations. MFI is the average brightness from all cells that are positive for the marker...Prathima has a research background in protein mutagenesis and cancer immunotherapies. She is currently ...

...involved every Addgenie in the company. Our Finance, Business and Legal teams had to pave the way for this project...support for export and shipping on dry ice. The Business and Finance Teams continue to facilitate the formation...

...microscope has been a powerful tool in almost all disciplines. In biology, innovative new imaging approaches...shows an image of differentiating monocyte with planes and hardware to it. Right panel shows workflow ...

..., a large set of headphones can act as a Do Not Disturb sign, even if the headphones aren’t actually playing...

...inhibition library that inhibits over 18,000 human genes and is used for genome-wide inhibition screening... of the extensive supply of transgenic reporter lines that supply a variety of GFP expression patterns...

...Ramsden DA (2014) The fidelity of the ligation step determines how ends are resolved during nonhomologous end...Protocol for Genomic Deletions in Mammalian Cell Lines [Video] Pooled CRISPR Libraries Offer Genome-Wide...

...complete. The nanobody based RANbody platform from the Sanes Lab overcomes this limitation and allows for the...its sequence in the comments below. References Sanes, J.R., & Yamagata, M. (2018). Reporter-nanobody ...

...we wanted to walk through some of our favorite milestones and share some thoughts from the community that... From day one, we built systems for now-common business practices, like accepting e-signatures and designing...

...but this advantage likely won’t transfer to all genes we’d like to target. In addition, as on-target editing...about High Fidelity CRISPR Variants Target Multiple Genes at Once with CRISPR Resources on Addgene.org Browse...

...essential to verify a number of key properties (brightness, photostability, oligomerization) of a number...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular...

...GFP-tagged Atg8p or LC3: ATG8 is part of a group of genes that affect autophagy (ATG) in yeast. Atg8p (“p”...stands for protein) associates with autophagosomal membranes, so tagging it with GFP allows for tracking the...

... CRISPR having effects on various biological disciplines, the base technology itself is constantly improving...collaboration with the Joung Lab, they used targeted mutagenesis to create HypaCas9 (SpCas9 N692A/M694A/Q695A/...

...silence–but it was thorough and coherent.  To be honest, if my paper hadn’t been ridiculously more complex... in front of a very confused group of visiting Chinese undergrads. You get nervous and freeze, or forget...

...acid, proteins containing two or more adjacent histidines are present in most production systems and can...can affect protein function. B, The string of histidines in the tag is typically between 6 to 9 residues...

...transparency Another major benefit of preprints is their openness. Preprints can be read freely by anyone and are... work too openly too soon or simply a lack of awareness of the benefits of preprints. Some worry about...

...you cannot test them all, so how do you know which ones to try?  Read on and we will provide you with some...in knock-out or knock-down down tissues and cell lines; orthogonal strategies that compare antibody assay...

...methods are often significantly longer than direct ones.  Even though the secondary antibody incubation ...to improve direct methods and avoid the indirect ones. Some Western blots, for example, use a primary ...

...versatile method to identify the expression of transgenes of interest. Furthermore, fluorophore variants...resulted in abundant expression of hM4Di in plasma membranes in both species (bottom panel in Fig. 1). Our ...

... (ChRs) makes them light-responsive. When light shines on excitatory channelrhodopsin-expressing neurons...channelrhodopsins tools have low conductance, but also confines channelrhodopsin activation to a small volume ...

...words —> decreasing frustration —> increasing the happiness. My unofficial motto in my Product Manager role...effective approaches for clearing up communications lines in your professional life, let me know that, too...

...can be the best way to get in touch if you have business or follow up. Use the "Contact info" link right...or comment on anything that is not relevant to business or volunteer organizational work. LinkedIn is ...

...time. Searching for literature My go-to search engines for finding scientific literature are Google Scholar...and download what seems interesting or relevant. Harness the power of the Google search engine and sign ...

...you through to a successful outcome and the good ones take this responsibility seriously. As I make my...Vivo Group whose tagline is Bridging Science and Business. Maybe you just need help getting through your...

..., and as we all know, a structural component of bones and teeth. It is an enzyme co-factor, a crosslinking...with other measurements, specifically fluorescent ones? Many of the available biosensors use a single GFP...

... C. elegans embryo when all essential embryonic genes are individually depleted via RNA interference (...that can bind to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In ...

...Since polyclonal antibodies contain a variety of clones and thus the potential to bind many different sites...secondary antibodies rather than generally reactive ones. And make sure to check for any reported cross-reactivity...

...genomes, scientists can control expression of their genes of interest. While recombinases are powerful gene... knockout library that targets human epigenetic genes was deposited by Kivanc Birsoy. Find the library...

...Tips for designing your poster Check conference guidelines To begin, you should always follow conference...we’ve just presented some standard formatting guidelines for making a scientific poster, you shouldn’t...

...fiber optics need to be polished to significant smoothness to prevent drastic loss of efficiency between...diamond polishing paper of sequentially increasing fineness helps to maintain a consistent stream of light...

...more I realized that it cuts across multiple disciplines, which really epitomizes what the makerspace ...Caenorhabditis Genetics Center (CGC) at the University of Minnesota has declared our makerspace an educational institution...

...proteins both transiently or through stable cell lines. Both methods produce high protein yields if transfection...cells)  that allow for continuous expression of genes integrated into the insect cell genome. Both of ...

...fewer transcripts of their antibiotic resistance genes and can therefore be cultured in media containing.... Spiking your media with supplements such as magnesium salts, buffering agents, and/or supplying additional...

...and test them in their labs. Figure 2: Guidelines to predict pegRNA efficiencies with pridict.it...any prime editor experimentalist, and especially shines once large-scale prime editing experiments come...

...other colonies containing smaller, recombined backbones that may replicate faster. For this reason, if...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

...CRISPR plasmids categorized by year of deposit. Outlines show a representative proportion of CRISPR plasmids...of tools seen in Figure 3, indicates that the usefulness of popular CRISPR tools is both enduring and ...

... the hierarchy of web pages the plasmid page is nested in. You can see in the example above that the bread...Database has more information about common vector backbones.  The backbone modifications listed can refer ...

...that could directly impact patients. And, to be honest, I’ve never enjoyed grant-writing. The more I talked...academia, however, writing papers and grants is an inescapable and essential part of being able to continue ...

...provides the AAV replication (rep) and capsid (cap) genes. AAV replication uses the host’s polymerase, but... the pHelper plasmid which expresses adenovirus genes which help mediate AAV viral replication; and 3)...

...can be identified. Even if some mammalian cells lines have similar morphologies, being familiar with these...Twice a week often works for most fast-growing cell lines (such as HEK293, which multiplies every 16 hours...