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  2. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...focus on development, along with the inherent trickiness of using and optimizing GCE, led to a large toolbox...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...

  3. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...microscope has been a powerful tool in almost all disciplines. In biology, innovative new imaging approaches...shows an image of differentiating monocyte with planes and hardware to it. Right panel shows workflow ...

  4. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...inhibition library that inhibits over 18,000 human genes and is used for genome-wide inhibition screening... of the extensive supply of transgenic reporter lines that supply a variety of GFP expression patterns...

  5. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...interviews on Al Jazeera and NPR, articles in magazines like Fast Company, opportunities to speak at conferences...be wondering how to get started. This blog post outlines 10 steps to take when starting to use Twitter ...

  7. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...involved every Addgenie in the company. Our Finance, Business and Legal teams had to pave the way for this project...support for export and shipping on dry ice. The Business and Finance Teams continue to facilitate the formation...

  8. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...Ramsden DA (2014) The fidelity of the ligation step determines how ends are resolved during nonhomologous end...Protocol for Genomic Deletions in Mammalian Cell Lines [Video] Pooled CRISPR Libraries Offer Genome-Wide...

  9. Summer SciComm: Preprints

    Type
    Blog Post
    ...Transparency Another major benefit of preprints is their openness. Preprints can be read freely by anyone and are... work too openly too soon or simply a lack of awareness of the benefits of preprints. Some worry about...

  10. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...you cannot test them all, so how do you know which ones to try?  Read on and we will provide you with some...in knock-out or knock-down down tissues and cell lines; orthogonal strategies that compare antibody assay...

  11. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...methods are often significantly longer than direct ones.  Even though the secondary antibody incubation ...to improve direct methods and avoid the indirect ones. Some Western blots, for example, use a primary ...

  13. Addgene: The First Twenty Years

    Type
    Blog Post
    ...we wanted to walk through some of our favorite milestones and share some thoughts from the community that... From day one, we built systems for now-common business practices, like accepting e-signatures and designing...

  14. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...silence–but it was thorough and coherent.  To be honest, if my paper hadn’t been ridiculously more complex... in front of a very confused group of visiting Chinese undergrads. You get nervous and freeze, or forget...

  15. To Each HIS Own

    Type
    Blog Post
    ...acid, proteins containing two or more adjacent histidines are present in most production systems and can...can affect protein function. B, The string of histidines in the tag is typically between 6 to 9 residues...

  16. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...words —> decreasing frustration —> increasing the happiness. My unofficial motto in my Product Manager role...effective approaches for clearing up communications lines in your professional life, let me know that, too...

  17. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...complete. The nanobody based RANbody platform from the Sanes Lab overcomes this limitation and allows for the...its sequence in the comments below. References Sanes, J.R., & Yamagata, M. (2018). Reporter-nanobody ...

  18. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...GFP-tagged Atg8p or LC3: ATG8 is part of a group of genes that affect autophagy (ATG) in yeast. Atg8p (“p”...stands for protein) associates with autophagosomal membranes, so tagging it with GFP allows for tracking the...

  19. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...but this advantage likely won’t transfer to all genes we’d like to target. In addition, as on-target editing...about High Fidelity CRISPR Variants Target Multiple Genes at Once with CRISPR Resources on Addgene.org Browse...

  22. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...can be the best way to get in touch if you have business or follow up. Use the "Contact info" link right...or comment on anything that is not relevant to business or volunteer organizational work. LinkedIn is ...

  26. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...genomes, scientists can control expression of their genes of interest. While recombinases are powerful gene... knockout library that targets human epigenetic genes was deposited by Kivanc Birsoy. Find the library...

  27. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ..., and as we all know, a structural component of bones and teeth. It is an enzyme co-factor, a crosslinking...with other measurements, specifically fluorescent ones? Many of the available biosensors use a single GFP...

  28. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...Since polyclonal antibodies contain a variety of clones and thus the potential to bind many different sites...secondary antibodies rather than generally reactive ones. And make sure to check for any reported cross-reactivity...

  30. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ... C. elegans embryo when all essential embryonic genes are individually depleted via RNA interference (...that can bind to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In ...

  31. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...fiber optics need to be polished to significant smoothness to prevent drastic loss of efficiency between...diamond polishing paper of sequentially increasing fineness helps to maintain a consistent stream of light...

  32. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...more I realized that it cuts across multiple disciplines, which really epitomizes what the makerspace ...Caenorhabditis Genetics Center (CGC) at the University of Minnesota has declared our makerspace an educational institution...

  34. Pooled Library Amplifications

    Type
    Blog Post
    ...other colonies containing smaller, recombined backbones that may replicate faster. For this reason, if...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

  35. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...CRISPR plasmids categorized by year of deposit. Outlines show a representative proportion of CRISPR plasmids...of tools seen in Figure 3, indicates that the usefulness of popular CRISPR tools is both enduring and ...

  36. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...that could directly impact patients. And, to be honest, I’ve never enjoyed grant-writing. The more I talked...academia, however, writing papers and grants is an inescapable and essential part of being able to continue ...

  37. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...provides the AAV replication (rep) and capsid (cap) genes. AAV replication uses the host’s polymerase, but... the pHelper plasmid which expresses adenovirus genes which help mediate AAV viral replication; and 3)...

  38. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...SpCas9 (derived from the bacterium Streptococcus pyogenes). Consequently, fewer genetic tools for targeted...nuclease-competent Cas9, obviating the need to generate new cell lines or transgenic animals expressing dCas9 as a means...

  39. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...fewer transcripts of their antibiotic resistance genes and can therefore be cultured in media containing.... Spiking your media with supplements such as magnesium salts, buffering agents, and/or supplying additional...

  40. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...can be identified. Even if some mammalian cells lines have similar morphologies, being familiar with these...Twice a week often works for most fast-growing cell lines (such as HEK293, which multiplies every 16 hours...
Showing: 641 - 680 of 905 results