...multiple recombinases can be used together to designate specific patterns of recombination. Finally, Rubin...

...He charged the postdocs to “Grab ahold of your destinies” and also suggested some concrete actions that...

...with genes knocked out in the insulin signaling cascades in only 4 months compared to a year or greater...

...already made for them,” Baird said. “They are all designed in the same vector backbone with very similar ...

...deposited at Addgene by over 250 scientists in Europe. Despite these fantastic numbers, a lot of European scientists...

...entertainment, politics, K-12, etc.” Addgene's team includes many PhD scientists who have stepped away from...

...results. GeoCas9 RNP editing in mammalian cells Besides being able to take some heat, GeoCas9 can introduce...

...These sensors generally follow the same pattern described above - a sensing domain induces changes in a ...

...should also be easily understood and consistent in design and layout. 2. Be concise in your writing  When...

...distributed more than 100 times. We hope these desktop trophies will serve as badges of honor for our ...

.... Traditional restriction cloning is one of the oldest of these techniques and is a multi-step process...

...spontaneous swimming. References Arrenberg, Aristides B., Filippo Del Bene, and Herwig Baier. "Optical...

...plastic is a substantial portion of lab waste. Despite the fact that researchers represent a minority ...

...References: del Valle Rodríguez A, Didiano D, Desplan C (2011) Power tools for gene expression and clonal...

...and morale. Especially when your collaboration includes scientists working in different time zones, it...

... to succeed. As a team, we talked through slide design, discussion items, big takeaways, and timing. The...

...onset) may be best served by this type of targeted design. Alternatively, a disease model can be generated...

...Each trap uses a slightly different approach (described in detail below), but fundamentally the reporter...

...available in the Addgene viral vector repository! This includes the first Addgene prep packaged in the AAV9-X1.1...

...focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with actionable steps...

... with my favorite microorganism (Rhodobacter sphaeroides!) at the ASM booth. As well as being the Director...

...Career: Doer, Manager, Leader” by Charlotte Beers, divides workers into three cycles: Doers, Managers and ...

...best? What 3 things worked least well? Manager provides feedback (may be the same as self-assessment or...

...research, SciFund is really motivated more by the desire to help close the widening gap between science ...

...Gel Procedure Primer Design and PCR Design Primers. See our protocol on how to design primers . Note: (Link...sequence. FAQ How do I design primers? See our reference page on how to design primers for detailed instructions...Description of Polymerase Chain Reaction with protocol, tips and FAQ Protocols...DNA and the Taq polymerase, PCR requires free nucleotides [dNTPs; adenine (A), cytosine (C), guanine (G...the denaturing. Your 5’ and 3’ primers should be designed to have similar melting temperatures (Tm). Set...protocol on plasmid cloning by PCR to learn how to design primers for cloning purposes. What do I do if my... to work efficiently. Taq Buffer with MgCl 2 : Provides an optimal and stable chemical environment for...

... 1: Buffer exchange using a desalting column The 10 mL Zeba Spin Desalting Columns can accommodate up ...This protocol describes how to affinity purify recombinant antibodies from cell culture supernatant using...Western Blot Protocol Introduction This protocol describes how to affinity purify recombinant antibodies ...L2884 Aprotinin, Millipore Sigma A6279 Zeba Spin Desalting Columns, 7 kDa MWCO, 10 mL, Thermo Fisher 89893... buffer exchange using a Zeba Spin 7 kDa MWCO desalting column. If the concentration of the pooled sample...into 2 columns. Twist off the 10 mL Zeba Spin Desalting Column’s bottom closure and loosen cap. Place ...Spectrophotometer to see if the sample has reached the desired concentration. If the sample concentration is still...

...needed for TOPO cloning: 1. Create Your PCR Product: Design standard primers (no need to add unique restriction...

...someone how and why they left academia. Humans are designed to pay attention and enjoy anecdotal forms of ...

...your institution’s tech transfer professionals: Describe to your TTO how unprocessed agreements slow down...

...activity in the brain was measured with implanted electrodes. The Archon1 voltage reporter allows a way to...

...dynamic process into a static measurement and it provides limited information about what materials or organelles...

...of 173 known synthetic and natural genes.  In designing and optimizing their algorithm, they found that...

... A -C compatibility grouping is an arbitrary designation, and plasmids from the same incompatibility group...

...harder to build relationships when you are more desperate or needy so you can’t wait until you imminently...

... enhances productive collaborations as well as provides visibility for grant-driven research being done...

...this post, you’ve got the knowledge to be able to design a construct for the successful and specific expression...

...H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both...

...immunocytochemistry (ICC). Not all of the solutions described here may be as effective for other techniques,...

...In the case above, you can’t costain with your desired antibodies because the pair belong to the same ...

...and workflows where CRISPR nuclease activity is desired and time or protocol steps can be saved by flexibly...

...opposing forces or (2) the result of competing desires or (3) the presence of obstacles that need to be...

...colleagues started with the FP mNeptune684 (originally described by Li ZY, et al. PLoS One, 2016) and tested a ...

... own experiences founding and running miLab to describe some key lessons I learned while becoming a health...

...Coordinator’ came up at Abcam. Unlike other job descriptions for marketing positions posted on recruitment...

...beginnings of the field. Those engineered sensors were designed to replace synthetic calcium dyes, which had been...

...download the "Mentoring for Scientists" ebook, which includes the entire series of mentoring blog posts, the...

...how can future regulations impact your work? What design variables need consideration? Enrich your knowledge...

...oligo overlap cloning, you can design a set of oligos containing your desired restriction sites and add them...that will pay off for years to come. Design Briefly, we will design overlapping oligos that once annealed...procedure will simply differ in terms of primer design). Let's assume that your favorite vector has a ...digest of existing sites in the original vector. Designing oligos To add NdeI, PacI, AscI and MfeI sites ...between the EcoRI and SalI sites of the vector, we design a top oligo with each of the additional sites in...complementary C of the other oligo), but this would destroy the EcoRI and SalI sites in the final vector. Order...

...This protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected...Protocol Antibody Guide Introduction This protocol describes how to run an indirect Enzyme-Linked ImmunoSorbent...antigen to validate an antibody. This protocol will describe how to create a standard curve using known concentrations...antibody and an isotype control antibody to the desired concentrations in the antibody dilution buffer....HRP-conjugated isotype-specific secondary antibody to the desired concentrations in the antibody dilution buffer....Check the plate periodically to determine when the desired color change has occurred. The color will change... to optimize experimental conditions. When the desired color change has occurred, gently remove the plate...

...gel Watch the Video! How to Design a Primer Key considerations when designing primers Watch the Video! Sequence...Intro to the Lab Bench Introductory techniques designed to help you get started in the lab. Basic Molecular...antibody applications. Intro to the Lab Bench Name Description (Link opens in a new window) Link to Video Personal...materials on a balance Basic Molecular Biology Name Description (Link opens in a new window) Link to Video Making...without a commercial kit Plasmid Cloning Name Description (Link opens in a new window) Link to Video Restriction... targeting human and mouse genes. Virus Name Description Link to Video General Transfection Introduce ... your lentivirus preparation Antibodies Name Description Link to Video Transfection Introduce plasmid ...