We narrowed to 964 results for: app

1. What's New in CRISPR - Spring 2019

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   Blog Post

   ...inhibitors Using a bioinformatics and a screening approach, the Jennifer Doudna lab identified three different...

2. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

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   Blog Post

   ...easy to multiplex. Their design allows flexible swapping of fluorescent proteins and multiplexing is simple...

3. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

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   Blog Post

   ...Chapter 8: The importance of a mix of research approaches. Why researchers should have a mix of high-risk...

4. Your Top Requested Plasmid in 2016!

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   Blog Post

   ...generation of a gRNA that will target SpCas9 to the approrpriate cleavage site). Finally, in addition to the ...

5. Stepping into a Virtual Internship: My Experience at Addgene

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   Blog Post

   ... my first day and meet everyone but this never happened. I actually started at a later date and worked...

6. Reflections and Looking Ahead

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   ...milestones remind us of the extraordinary impact that happens when scientists come together to share their work...

7. What's New in CRISPR - December 2019

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   Blog Post

   ... toolkit named ORANGE (Open Resource for the Application of Neuronal Genome Editing) mediates targeted...

8. Editor's Choice, August 2016

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   Blog Post

   ...suggestions for us, feel free to comment below. Happy reading! Tyler J. Ford ...

9. Management for Scientists: Seeking Feedback

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   Blog Post

   ...feel they are wasting their time. The manager may appear to be too busy to be receptive.   Whatever the ...

10. Scientist Networking: What is an Informational Interview?

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    Blog Post

    ...opportunities for advancement, challenges and disappointments. These more personal questions can elicit the...

11. X-CHIME: Context Dependent Germline Knockout in Immune Cells

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    ... Figure 1: Schematic showing the X-CHIME approach. Figure from LaFleur, et al. 2024.   The X-CHIME...

12. SciComm with the Experts at Science in the News Part 2

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    Blog Post

    ...specific topics discussed during the interview. Happy listening! Listen to the interview with Science ...

13. Easing the Protein Purification Process with pCri

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    Blog Post

    ...comprehensive review of affinity tags and microbial applications." Biotechnology journal 7.5 (2012): 620-634....

14. Inside Addgene's 10 Year Anniversary Gala

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    Blog Post

    ...Years of Plasmid Sharing Or read more about what happens Inside Addgene   ...

15. Celebrating Accomplishments in the Lab

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    Blog Post

    ... few of those have been trips to breweries who happily exchange our expertise with lots of free beer.”...

16. Three Tips to Organize Your Lab Notebooks in the New Year

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    Blog Post

    ... used protocols & references easy to find It’s happened to all of us. It’s your first day back in the ...

17. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

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    Blog Post

    ...it is Yoda who is singled out as a bad mentor. Apparently, a good Jedi does not necessarily an excellent...

18. Tag Your Favorite Yeast Genes with Ease

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    Blog Post

    ...containing the modifying sequence flanked by approximately 50 base pairs of sequence homologous to the ...

19. Scientific Sharing in the Time of COVID-19: Databases and Resources

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    Blog Post

    ...is based in Canada, much of the information is applicable to researchers worldwide. “COVID-19 has spread...

20. 2023 and 2024: Reflections and Looking Ahead

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    Blog Post

    .... Through an expansion of our export license application service, we’re pleased to announce that we are...

21. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

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    Blog Post

    ...). IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells...

22. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

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    Blog Post

    ...experiment, that you can find what you need and feel happy and inspired to tackle your next science challenge...

23. Cre-ating New Methods for Site-specific Recombination in Drosophila

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    ...tubulin-GAL4. For Dre, Flp, KD, R, B2 and B3, approximately 50% of offspring carried both the recombinase...

24. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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    Blog Post

    ...YFP) to the new and improved fruit colors (e.g., apple, papaya, and tomato) and the photoactivatable fluors...

25. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

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    Blog Post

    ... lentiviral libraries and their demonstrated application offer proof-of-principle that CRISPR-Cas9 can...

26. What's the Best Way to Elute and Store Your Plasmid DNA?

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    Blog Post

    ...directly use your DNA preparation in any downstream application involving enzymatic reactions, a valid alternative...

27. Plasmids 101: What is a plasmid?

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    Blog Post

    ...broadly utilize plasmids across a wide range of applications. Some common plasmid types include cloning plasmids...

28. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

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    Blog Post

    ...resistance genes, toxin genes, and the sacB gene). By tapping into nature’s toolbox, scientists are finding more...

29. Sensing Neuronal Dopamine

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    Blog Post

    ...neurotransmitter. But aside from DA’s famous role as the “happy hormone,” it’s also involved in reinforcement learning...

30. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

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    ...spontaneous swimming  Researchers at the Isacoff lab applied optogenetics in zebrafish larvae to identify new...

31. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

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    Blog Post

    ...Commons 3.0 license, which requires users to give appropriate credit, provide a link to the license, and indicate...

32. Antibodies 101: Fc Effector Functions

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    ...determination, don’t generally affect antibody applications like ELISAs or Western blots, they are key in...

33. CRISPR 101: Drug discovery and CRISPR

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    ...discovery Present drug discovery takes one of two approaches. The first route involves identifying a gene/...

34. Cellosaurus: A Cell Line Information Database

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    Blog Post

    ...cells lines. A 2014 study in Science found that approximately one-third of cell lines used in scientific research...

35. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

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    Blog Post

    ... experiment to demonstrate the most exciting application for ARG’s: in vivo imaging. Specifically, arg1...

36. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

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    ... a Cas9 nickase and two gRNAs.  For nickase applications, a common question is: how should the gRNAs ...

37. Oh, The Places You Can Go: Careers in Science Communication - Product Development

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    Blog Post

    ...upon eScience by luck, by chance, by complete happenstance. It was on Indeed or one of the job search sites...

38. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

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    Blog Post

    ... and maintaining confidentiality where it is appropriate. This grows our relationship with our community...

39. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

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    Blog Post

    ...making it accessible to students.” Making science approachable is often the most challenging part of the job...

40. Bricking Science: Portraying Scientific Reality Through LEGO

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    Blog Post

    ...motion format for our videos as it is the most approachable for LEGOs. Stop motion consists of taking many...

41. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

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    Blog Post

    ...publication are also provided for the user. This approach serves to maintain consistency with published ...

42. Networking and Inspiring Young Scientists at Freshers Fairs

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    Blog Post

    ...sessions to help undergraduate students with their applications. The society currently has 1,283 members, who...

43. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

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    ...follow your passion and land a position you’ll be happy in once you’ve finally acquired that PhD. Resources...

44. 10 Ways to Share Your Science!

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    ...Morehead Planetarium. Similar things are likely happening in your community, and, if they aren’t, think ...

45. Choosing a Good Mentor for Scientists

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    ...away. Many scientists think, “Oh, but that won’t happen to me – I am different.”  Unfortunately, a bad ...

46. Summer SciComm Series: Master of SciComm

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    ...science communicator—with an understanding of how to appeal to a given audience and how to utilize a wide array...

47. RUBY-Red Siliques

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    ...culture), which is great for a wide variety of applications. But some techniques require you to select against...

48. Hot Plasmids and Viral Preps - September 2021

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    Blog Post

    ...several MYC-dependent cancer cell lines. It can be applied for identification of essential MYC binding sites...

49. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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    Blog Post

    ...reached the level of expression needed for most applications.   Figure 1: With blue light, the VVD...

50. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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    Blog Post

    ...which can limit expansion into new services. “We appreciate NIH’s vision and support for this project. This...