We narrowed to 658 results for: CHI;

...engineered Cas9 variant might work for your experiment. PITChing MMEJ as an Alternative Route for Gene Editing ...challenging, with protocols using dsDNA templates achieving ~1-10% efficiency. Easi-CRISPR uses a long ssDNA...

...Glick from SnapGene!  Ben Glick is President and Chief Scientist at SnapGene, and also a Professor of Molecular...Genetics and Cell Biology at the University of Chicago. He was an early depositor at Addgene, and conceived...

...work. A younger interest in being a writer kept chirping up. The evolutionary perspective brought by studies...with authors and referees. And I am constantly teaching – often explaining a technical piece of research...

...accompany him to YNP the following year. Geoff Schiebinger, another Quake Lab member joined for half of ...in YNP just as the weather was starting to turn chilly at the 8,000 foot elevation. Due to the 3,471 square...

...zebrafish embryo over an 80 minute time course.  By stitching together a stable scaffold and HA-binding regions...protein dynamics in vivo. In the future, this stitching technique could be applied to other epitopes and...

...protein (LanYFP) from the marine invertebrate Branchiostoma lanceolatum in 2012. Using structural modeling...monomeric green fluorescent protein derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407...

...119741  AAV5  AAV SYN flex PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation 119742  AAV5... AAV5  AAV SYN PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation Recombinases Plasmid...

... examine biosynthetic pathways A new tool for enriching ABE-targeted cells The CRISPR Corner New ready-to-use...-boxes genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent cancer cell ...

...sgRNAs, but they may or may not include CRISPR machinery (which can be supplied separately). Libraries ...systematically explore variations in the spike protein, teaching us more about viral evolution and antibody binding...

...disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they enter a cell...delivered to the cell. The cell then uses its own machinery to replicate the viral genome and proteins. This...

... research, we hope we can help all researchers achieve happiness in their careers regardless of their ...by non-homologous end joining (NHEJ). 7. Career Coaching for Scientists: Why and Where Do I Find One? ...

...things in parallel.” Alternating Hemiplegia of Childhood Melodi McSweeney (right), a graduate student at...the disease known as Alternating Hemiplegia of Childhood (AHC). Patients affected by AHC suffer from recurrent...

...complicated (and again, expensive) the different machines are, and how many regulations are in place to ... on the Addgene Blog Find planarian tools for teaching developmental biology Use the pathways over time...

...courses on genetic manipulation more hands on While teaching courses about gene therapy and genetic manipulation... on the Addgene Blog Find planarian tools for teaching developmental biology Learn how BioBuilder can...

...translated RNAs appear red. To enable this color switching, TRICK requires the following components: a GFP... specificity and resolution of this system are achieved by using multiple copies of the PP7 and MS2 coat...

...resting state and its calcium-bound state, which was achieved by lowering the basal fluorescence of the sensor...inhibiting neurons. Minimally-tagged DREADD AAV from the Richie lab are especially useful for studies in non-human...

...dropped a bombshell report. A scientist working in China was using CRISPR/Cas9 in an attempt to create gene-edited...claim has yet to be independently verified, the watching world erupted in controversy. Briefly, there are...

...free interaction and brainstorming. This could be achieved by asking each presenter to come up with a scientific...Google Docs and Sheets during Zoom lab meetings to chip in ideas and take minutes that can be accessed by...

...Alzhiemer’s disease (AD).  Troy Rohn and his lab are researching a way to treat AD using CRISPR/Cas9. They are...diseases In 2013, a team led by Jinsong Li from the Chinese Academy of Sciences found that mice with a dominant...

...pharmaceutical companies is K. phaffii (formerly known as pichia). What is this new-fangled yeast? It’s actually...specifically glycosylation. The native glycosylation machinery of K. phaffii lacks certain terminal mannose glycopeptides...