... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. Today, it'...or inaccessible. The Ting lab recently made two deposits that increase the utility of this technique.  ...

...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...recipient’s initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...

...even been linked to some diseases. It has been reported that 5-10% of human genes contain a region where...EMBO Rep 1:18–23 . https://doi.org/10.1093/embo-reports/kvd001 Additional resources on the Addgene blog...

...of interest and an inactivated fluorescent RNA reporter. If the target sequence is present in the pool...activity of Cas13a becomes activated and the RNA reporter will be cleaved resulting in activation of the...

...single-use aliquots, and stored at -80°C. Some reports suggest rapidly freezing virus in a dry ice/ethanol...Things Lentivirus with our Lentiviral Guide Pages Deposit Your Lentiviral Vectors ...

...may be a plasmid that constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either... well of your cells. Regardless, the amount of reporter protein activity correlates to both the amount...

... where two proteins are fused to complementary reporter proteins that can only assemble when in close ... ascorbate peroxidase as a genetically encoded reporter for electron microscopy." Nature biotechnology...

...discovery of RNAi in the worm actually rewarded depositors Andrew Fire and Craig Mello a Nobel Prize in ...important gene co‑expression modules." Biomedical Reports 7.2 (2017): 153-158. PubMed PMID: 28804628. PubMed...

... the Confetti mouse (Snippert et al., 2010), a reporter mouse that can be used to track any cell type ...XFP expression in the Ubow mouse Another murine reporter animal, the Ubow mouse (Ghigo et al., 2013), was...

...interest with an additional protein module that can report on location. This approach enables the study of...9759496.  3. Buckley, Anthony M., et al. "LOV-based reporters for fluorescence imaging." Current opinion in ...

...great point when I then ask “and who has deposited to the repository?” When most or all of the hands go down...

...Susanna Bachle, Addgene the nonprofit plasmid repository 49:45 0 1:18:45 - Reagent Development - Steven...of reproducibility for preclinical studies, as reported by drug companies attempting to apply the findings...

... it a single nucleotide change, insertion of a reporter gene, or replacement of the murine sequence with... R (2013) One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated...

...distributed throughout the genome. We encourage you to deposit your piggyBac™ transposon vectors with us to help... up on Everything CRISPR with Our CRISPR Guide Deposit Your piggyBac™ Vectors! ...

...piggyBac plasmids    Tol2  Tol2 was the first reported active DNA transposon in vertebrates. It was discovered...mutations. In these screens, the transposons encode reporter genes, mutagenic cassettes, or barcodes. When ...

... unbiased methods, high background BLESS Not reported Cell-based (in vitro) Can be used on tissue from...senstive to time since cell fixing HTGTS Not reported Cell-based Identifies translocations Limited by...

... self-labeling tools: Tetracysteine: The first reported chemical labeling tag, from Roger Tsien’s lab ...collection. CLIP-tag: Also in 2008, Kai Johnsson’s lab reported another AGT-based tag named CLIP-tag, which reacts...

...gene (e.g., channelrhodopsin-2, ChR2) fused to a reporter gene (e.g., mCherry) to easily detect opsin-expressing...recombinase in mouse primary afferents." Scientific reports 8.1 (2018): 7321. PubMed PMID: 29743652. PubMed...

...transgenic mice expressing fluorescent protein reporter constructs in the immune lineage. By specifically...using 2-photon microscopy as this hasn't been well reported for many fluorescent proteins. Additional fluorescent...

...Plasmid 68285 SepOTSδ Plasmid 68286 SepOTSϵ Plasmid 68287 SepOTSζ Plasmid 68288 SepOTSη Plasmid 68289 SepOTSθ...68290 SepOTSι Plasmid 68291 SepOTSκ Plasmid 52054 SepOTSµ ( aka. B40 OTS) Plasmid 68294 SepOTSν Plasmid...Plasmid 34624 SepOTSα tRNA-Sep ( aka. pCAT112TAG-SepT) Plasmid 68283 SepOTSβ Plasmid 68284 SepOTSγ Plasmid ....Δserb.Amp S Plasmid 68292 SepOTSλ Plasmid 68307 SupD Plasmid 34623 SepOTSα SepRS/EF-Sep ( aka. pKD-SepRS-EFSep... Depositor Collections Rinehart Lab Phosphoprotein Reagents...188537 ) a better choice in place of the popular SepOTSλ ( 68292 ). An important technical note: pSerOTS-C1...can be generated with either phosphoserine using SepOTSλ ( 68292 ) or serine using supD tRNA ( 68307 ) at...