Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 658 results for: EPO;

Showing: 321 - 340 of 658 results

  1. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Hu et al., 2018).  In 2020, the Kleinstiver lab reported the development of near-PAMless Cas9 variants....Streptococcus thermophilus (St) NNAGAAW Treponema denticola (Td) NAAAAC ~20 additional Cas9...

  3. How to Write a Scientific Review Article

    Type
    Blog Post
    ... member of a class of proteins was mutated, as reported in various studies. We made a table that listed...for each protein we listed all the studies that reported mutations in that protein (including how frequently...

  4. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...single-use aliquots, and stored at -80°C. Some reports suggest rapidly freezing virus in a dry ice/ethanol...Things Lentivirus with our Lentiviral Guide Pages Deposit Your Lentiviral Vectors ...

  5. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...may be a plasmid that constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either... well of your cells. Regardless, the amount of reporter protein activity correlates to both the amount...

  6. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...interest with an additional protein module that can report on location. This approach enables the study of...9759496.  3. Buckley, Anthony M., et al. "LOV-based reporters for fluorescence imaging." Current opinion in ...

  8. Five Popular Model Organisms

    Type
    Blog Post
    ...discovery of RNAi in the worm actually rewarded depositors Andrew Fire and Craig Mello a Nobel Prize in ...important gene co‑expression modules." Biomedical Reports 7.2 (2017): 153-158. PubMed PMID: 28804628. PubMed...

  10. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...great point when I then ask “and who has deposited to the repository?” When most or all of the hands go down...

  12. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ... it a single nucleotide change, insertion of a reporter gene, or replacement of the murine sequence with... R (2013) One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated...

  13. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...distributed throughout the genome. We encourage you to deposit your piggyBac™ transposon vectors with us to help... up on Everything CRISPR with Our CRISPR Guide Deposit Your piggyBac™ Vectors! ...

  14. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... self-labeling tools: Tetracysteine: The first reported chemical labeling tag, from Roger Tsien’s lab ...collection. CLIP-tag: Also in 2008, Kai Johnsson’s lab reported another AGT-based tag named CLIP-tag, which reacts...

  15. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...piggyBac plasmids    Tol2  Tol2 was the first reported active DNA transposon in vertebrates. It was discovered...mutations. In these screens, the transposons encode reporter genes, mutagenic cassettes, or barcodes. When ...

  17. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ... unbiased methods, high background BLESS Not reported Cell-based (in vitro) Can be used on tissue from...senstive to time since cell fixing HTGTS Not reported Cell-based Identifies translocations Limited by...

  19. Luciferase Plasmid Collection

    Type
    Collection
    ... 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic ...Multiple Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-b, c-Myc...for gene expression assays and bioluminescent reporters. Plasmid...Collection Empty Backbones Expression Constructs Reporter Constructs Other Highlights You may also like....-lives, making them useful as transcriptional reporters, but are less-than-ideal protein tags due to their...or gene of interest into to create a luciferase reporter. Unless otherwise noted, these plasmids are meant...luciferase and renilla_polyA Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions...

  20. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...68289 SepOTSθ Plasmid 68288 SepOTSη Plasmid 68287 SepOTSζ Plasmid 68286 SepOTSϵ Plasmid 68285 SepOTSδ Plasmid...Plasmid 68294 SepOTSν Plasmid 68292 SepOTSλ Plasmid 68291 SepOTSκ Plasmid 68290 SepOTSι Plasmid 68289 ...Plasmid 68284 SepOTSγ Plasmid 68283 SepOTSβ Plasmid 53225 MBP-MEK1 S218TAG/S222TAG ( aka. PCRT7 tetR pLtetO...EcAR7 Plasmid 52054 SepOTSµ ( aka. B40 OTS) Strain 34929 BL21ΔserB Plasmid 34624 SepOTSα tRNA-Sep ( aka. ... Depositor Collections Rinehart Lab Phosphoprotein Reagents...#188537) a better choice in place of the popular SepOTSλ (Plasmid #68292) . An important technical note:...optimized phosphoserine orthogonal translation system, SepOTSλ (Plasmid #68292) , they were able to demonstrate...
Showing: 321 - 340 of 658 results