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Showing: 321 - 340 of 705 results
  1. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...process When I first saw the comprehensive job description for this co-op, I was immediately interested ...had stood out to me because it gave me a full description of the tasks I would be responsible for, in comparison...
  2. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...Cas9 and a donor vector to create knock-ins of fluorescent proteins, fusion proteins, or transcriptional...interest and on either side of the cargo (ex: fluorescent protein gene) on the donor vector. Therefore,...
  3. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...fascinating and fun look at her outreach work with fluorescent microbes. Check out her animation about the Hawaiian...repository (like these new Rainbow Bacterial Fluorescence Labeling Plasmids from the Barbier lab). Bright...
  4. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...Antibody Guide, which include information about immunofluorescence, conjugates, isotypes, and more. How You ...Basics of Western Blotting Introduction to Immunofluorescence Addgene’s Antibody Guide The project is ...
  5. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...Direct reprogramming describes the process where differentiated cells are turned into a cell type of ...reprogramming factors for generating iPSCs Learn how fluorescent proteins help researchers study single cell dynamics...
  6. A Tour of Addgene's Most Popular Pooled Libraries

    Type
    Blog Post
    ...to identify specific cells — as well as their descendants. This makes them useful for lineage tracing, ... applications. Cells can be identified using fluorescence, like in the pMuSIC library, or by sequencing...
  7. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...p15A B Relaxed pSC101 ~5 pSC101 C Stringent pBluescript ~300-500 ColE1 (derivative) and F1** A Relaxed...multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12;52(10):1828-37...
  8. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...and more details on how to play the game on the FRESCI blog. As I wrote in the blog post, BUILD YOUR PLASMID...Barcelona. He is also founder and consultant at FRESCI particularly interested in advanced in vitro models...
  9. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...s time, we’ve published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction...other labs at UCSF (the QCRG network coming to the rescue -- again!). Also, we’ve got to mention that our...
  10. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...state, which was achieved by lowering the basal fluorescence of the sensor. This “contrast” variant provides... studies in non-human primates, where fusion fluorescent proteins can affect DREADD trafficking and localization...
  11. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...producer cells and purification protocols often describe a process that begins with cell lysis to release...Central PMCID: PMC4104587. György, Bence, et al. "Rescue of hearing by gene delivery to inner-ear hair cells...
  12. Components of CRISPR/Cas9

    Type
    Blog Post
    ... of the major CRISPR lingo and will be able to describe the functions of the various CRISPR/Cas9 components...Bind to a guide RNA The guide RNA, which will be described in more detail below, enables Cas9 to cut a specific...
Showing: 321 - 340 of 705 results