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  3. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...p15A B Relaxed pSC101 ~5 pSC101 C Stringent pBluescript ~300-500 ColE1 (derivative) and F1** A Relaxed...multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12;52(10):1828-37...

  7. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...s time, we’ve published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction...other labs at UCSF (the QCRG network coming to the rescue -- again!). Also, we’ve got to mention that our...

  8. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...state, which was achieved by lowering the basal fluorescence of the sensor. This “contrast” variant provides... studies in non-human primates, where fusion fluorescent proteins can affect DREADD trafficking and localization...

  9. Improving Transduction Efficiency with Exosome AAVs

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    Blog Post
    ...producer cells and purification protocols often describe a process that begins with cell lysis to release...Central PMCID: PMC4104587. György, Bence, et al. "Rescue of hearing by gene delivery to inner-ear hair cells...

  10. "Build Your Plasmid": The Game - Play to Teach

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    Blog Post
    ...and more details on how to play the game on the FRESCI blog. As I wrote in the blog post, BUILD YOUR PLASMID...Barcelona. He is also founder and consultant at FRESCI particularly interested in advanced in vitro models...

  11. Components of CRISPR/Cas9

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    Blog Post
    ... of the major CRISPR lingo and will be able to describe the functions of the various CRISPR/Cas9 components...Bind to a guide RNA The guide RNA, which will be described in more detail below, enables Cas9 to cut a specific...

  12. Plasmids 101: Mammalian Vectors

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    Blog Post
    ...mammalian plasmid components, it may be useful to describe the means of introducing genetic material (such...the plasmid in the same way. Selection methods, described below, are typically employed when creating stable...

  15. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post
    ...lentiviral packaging methods. The three components described above (envelope, packaging, and transfer) are ...nomenclature of the plasmid backbone is meant to be descriptive of the components found within the backbone. ...

  18. Improved Plasmid Maps Powered by SnapGene

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    Blog Post
    ...differ by only a few nucleotides, such as the fluorescent proteins EGFP and mEGFP. Many of these features...the gene, the name of its gene product, and a description of its function. You can also click one location...

  20. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...immediately write a text document with a brief description of the directory contents, at a minimum. This...for a single use, save the script as originalName_descriptionOfEdit in the same directory where it was ...
Showing: 321 - 340 of 705 results