We narrowed to 703 results for: ESC;

...Two new human lentiviral CRISPR library systems described in companion papers in Science in December were...Additionally, a concurrent Nature Biotechnology article describes the development of a mouse lentiviral CRISPR library...

... optogenetic tool (CsChrimson) attached to a fluorescent indicator (tdTomato), as the effector. They also...expression in flies Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular...

...fascinating and fun look at her outreach work with fluorescent microbes. Check out her animation about the Hawaiian...repository (like these new Rainbow Bacterial Fluorescence Labeling Plasmids from the Barbier lab). Bright...

...p15A B Relaxed pSC101 ~5 pSC101 C Stringent pBluescript ~300-500 ColE1 (derivative) and F1** A Relaxed...multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12;52(10):1828-37...

...of my review - "The manuscript by ZZZ et al., describes ... The authors share a wealth of data on ... ...Additionally, you can ask that data and resources described in the manuscript are accessible to the community...

...Direct reprogramming describes the process where differentiated cells are turned into a cell type of ...reprogramming factors for generating iPSCs Learn how fluorescent proteins help researchers study single cell dynamics...

...Antibody Guide, which include information about immunofluorescence, conjugates, isotypes, and more. How You ...Basics of Western Blotting Introduction to Immunofluorescence Addgene’s Antibody Guide The project is ...

...to identify specific cells — as well as their descendants. This makes them useful for lineage tracing, ... applications. Cells can be identified using fluorescence, like in the pMuSIC library, or by sequencing...

...will not produce the fluorescent protein while areas that are exposed will fluoresce. With these images,...

...to bind fluorescent proteins derived from A. victoria the functionalized nanobodies described here can...

... a feature inaccurately. The best example is fluorescent proteins, which often come in closely related...misidentification, we augment the database with new fluorescent protein variants. The future of DNA feature ...

...as their kidneys fail. The condition was first described more than 60 years ago, but there is still no ...changes in temperature.  “The disease was first described in 1971,” McSweeney said. “But, it is still sometimes...

...published in Nature Methods, Boyden and his team describe the discovery of a light-sensitive opsin from ...then, there has been a steady stream of papers describing new photosynthetic or photosensory molecules ...

...state, which was achieved by lowering the basal fluorescence of the sensor. This “contrast” variant provides... studies in non-human primates, where fusion fluorescent proteins can affect DREADD trafficking and localization...

...producer cells and purification protocols often describe a process that begins with cell lysis to release...Central PMCID: PMC4104587. György, Bence, et al. "Rescue of hearing by gene delivery to inner-ear hair cells...

...s time, we’ve published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction...other labs at UCSF (the QCRG network coming to the rescue -- again!). Also, we’ve got to mention that our...

...and more details on how to play the game on the FRESCI blog. As I wrote in the blog post, BUILD YOUR PLASMID...Barcelona. He is also founder and consultant at FRESCI particularly interested in advanced in vitro models...

... of the major CRISPR lingo and will be able to describe the functions of the various CRISPR/Cas9 components...Bind to a guide RNA The guide RNA, which will be described in more detail below, enables Cas9 to cut a specific...

...mammalian plasmid components, it may be useful to describe the means of introducing genetic material (such...the plasmid in the same way. Selection methods, described below, are typically employed when creating stable...

...differ by only a few nucleotides, such as the fluorescent proteins EGFP and mEGFP. Many of these features...the gene, the name of its gene product, and a description of its function. You can also click one location...