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We narrowed to 645 results for: ESP

Showing: 321 - 340 of 645 results
  1. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post
    ...which protein fluors are likely to trigger immunoresponses. Nucleotides, in general, are far less immunogenic...small molecules that do not generate an immune response. Aptamers can be selected for these small ligands...
  2. Antibodies 101: Reading a Flow Plot

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    Blog Post
    ...Gern, J., & Seroogy, C. (2017). Neonatal immune response to rhinovirus A16 has diminished dendritic cell...Greiner, D., & Anderson, D. (2015). Neutrophil Responses to Sterile Implant Materials. PloS One, 10, e0137550...
  3. Viral Production at Addgene

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    Blog Post
    ...reported or posted on the material page for the corresponding catalog item (see maps section for images). ...targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease...
  4. Transferable Skills Guide: External Collaborations

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    Blog Post
    ...also very important in a non-research context, especially when considering small to mid-sized companies...When you reach out as a student, people tend to respond in a supportive way and are willing to help because...
  5. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...envelope protein on the virus’s surface and a corresponding receptor in the host cell. Some of these envelope-receptor...infecting neurons and cause minimal inflammatory response, but are not taken up by axon terminals and can...
  6. Finding nucleic acids with SHERLOCK and DETECTR

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    Blog Post
    ...neighboring ssRNA and ssDNA by Cas13 and Cas12a, respectively, to cleave and activate a reporter. The detectable...amplification) or reverse transcriptase (RT)-RPA, respectively. RPA is coupled with T7 transcription to convert...
  7. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    Blog Post
    ...experimental target, SpCas9 and its enhanced variants, eSpCas9 and HypaCas9, are great choices. Check out our ... using a Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9 for site-specific...
  8. Supporting Reproducibility with a Connected ELN

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    Blog Post
    ...supports reproducibility RSpace was designed in response to requirements that emerged from intensive engagement...developed with these principles in mind, and in response to further input provided by the research communities...
  9. Mesothelioma - Causes, Symptoms, and Treatment

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    Blog Post
    ...mesothelioma many years later. While there is a dose-response relationship (the higher the exposure, the greater...cell, and binds cells that can begin an immune response. This important biomarker can be used to distinguish...
  10. 5 Reasons to Use Reddit for Science Communication

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    Blog Post
    ...but like to be able to provide well thought out responses. I hung out with Joanne as she answered questions...toxic advisors, fruitless job searches, and even despair at having chosen an academic past. I hope I was...
  11. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    Blog Post
    ...lentiviruses lack a functioning integrase, the enzyme responsible for inserting viral DNA into the host genome....unwanted interruptions. Insertional mutagenesis is especially concerning in clinical settings, as evidenced...
  12. Antibodies 101: Introduction to Gating in Flow Cytometry

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    Blog Post
    ...as FSC-A/SSC-A, FSC-H/SSC-H, or FSC-W/SSC-W, respectively. H, A, and W describe the shape of a histogram...population into DN, DP and SP (for each stain respectively). (B) Instead of a quadrant gate, I am free ...
  13. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...Cell/Science paper. You do need multiple first/corresponding author publications in quality journals. It ... of guidance or the document examples are not especially helpful. I set out to make something practical...
  14. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post
    ...using the online calculator from the Salis group) (Espah Borujeni et al., 2014). Replaced the pDS132 MCS ...bacteria become resistant to antibiotics! References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard...
Showing: 321 - 340 of 645 results