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Addgene
Showing: 321 - 340 of 628 results

  1. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...counterparts. Those familiar with viral vectors, and especially those familiar with adeno-associated viruses ...chemogenetic tools: protein tools that are activated, respectively, by substrates, light, or engineered ligands...

  2. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...procedure is longer than alternative ELISA methods, especially when secondary antibodies are used.  Competitive...positives.  If testing a recombinant protein, especially one with tags, be sure to include a sample that...

  3. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...also very important in a non-research context, especially when considering small to mid-sized companies...When you reach out as a student, people tend to respond in a supportive way and are willing to help because...

  4. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Gern, J., & Seroogy, C. (2017). Neonatal immune response to rhinovirus A16 has diminished dendritic cell...Greiner, D., & Anderson, D. (2015). Neutrophil Responses to Sterile Implant Materials. PloS One, 10, e0137550...

  5. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...mesothelioma many years later. While there is a dose-response relationship (the higher the exposure, the greater...cell, and binds cells that can begin an immune response. This important biomarker can be used to distinguish...

  6. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...but like to be able to provide well thought out responses. I hung out with Joanne as she answered questions...toxic advisors, fruitless job searches, and even despair at having chosen an academic past. I hope I was...

  8. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...experimental target, SpCas9 and its enhanced variants, eSpCas9 and HypaCas9, are great choices. Check out our ... using a Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9 for site-specific...

  11. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...using the online calculator from the Salis group) (Espah Borujeni et al., 2014). Replaced the pDS132 MCS ...bacteria become resistant to antibiotics! References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard...

  13. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...Cell/Science paper. You do need multiple first/corresponding author publications in quality journals. It ... of guidance or the document examples are not especially helpful. I set out to make something practical...

  15. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...lentiviruses lack a functioning integrase, the enzyme responsible for inserting viral DNA into the host genome....unwanted interruptions. Insertional mutagenesis is especially concerning in clinical settings, as evidenced...

  16. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...targeted nucleases In an effort to combine the respective advantages of the AAV and CRISPR-based approaches...efficiencies in T-cells and hematopoietic stem cells respectively. Future researchers could apply this technique...

  17. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...as FSC-A/SSC-A, FSC-H/SSC-H, or FSC-W/SSC-W, respectively. H, A, and W describe the shape of a histogram...population into DN, DP and SP (for each stain respectively). (B) Instead of a quadrant gate, I am free ...

  18. Evolution of Lab Techniques

    Type
    Blog Post
    ...should ultimately contain radioactive bands that correspond to every nucleotide in your DNA sequence, based...Figure 2). Accuracy and accessibility led to the widespread use of Sanger sequencing. Building upon this ...

  19. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ... and these are all symptoms of a negotiation, especially when money is involved. However, it doesn’t have...how much do you need to start your lab”. You can respond by saying, “The exact start up costs may vary greatly...
Showing: 321 - 340 of 628 results