We narrowed to 639 results for: ESP
-
TypeBlog Post...Gern, J., & Seroogy, C. (2017). Neonatal immune response to rhinovirus A16 has diminished dendritic cell...Greiner, D., & Anderson, D. (2015). Neutrophil Responses to Sterile Implant Materials. PloS One, 10, e0137550...
-
Viral Production at Addgene
TypeBlog Post...reported or posted on the material page for the corresponding catalog item (see maps section for images). ...targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog Post...which, for example, the activity of all genes in response to an experimental treatment can be monitored....CRISPR/Cas9-mediated insertion of foreign DNA. Despite these caveats, several features of zebrafish—including... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...pathways generating interesting drug-like compounds especially cardenolides which have been used to treat heart... and one is promiscuous and the gene that is responsible is understood. It’s possible to actually silence... -
Supporting Reproducibility with a Connected ELN
TypeBlog Post...supports reproducibility RSpace was designed in response to requirements that emerged from intensive engagement...developed with these principles in mind, and in response to further input provided by the research communities... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...envelope protein on the virus’s surface and a corresponding receptor in the host cell. Some of these envelope-receptor...infecting neurons and cause minimal inflammatory response, but are not taken up by axon terminals and can... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post...which protein fluors are likely to trigger immunoresponses. Nucleotides, in general, are far less immunogenic...small molecules that do not generate an immune response. Aptamers can be selected for these small ligands... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog Post...mesothelioma many years later. While there is a dose-response relationship (the higher the exposure, the greater...cell, and binds cells that can begin an immune response. This important biomarker can be used to distinguish... -
5 Reasons to Use Reddit for Science Communication
TypeBlog Post...but like to be able to provide well thought out responses. I hung out with Joanne as she answered questions...toxic advisors, fruitless job searches, and even despair at having chosen an academic past. I hope I was... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post...are many reasons why client situations might be especially challenging. For instance, the client might need...at business schools, and consulting clubs are especially helpful since they both provide up-to-date information... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...determine this is to examine expression of the early response gene c-fos. For example, using Chr2 stimulation...cue. The hippocampus has long been known to be responsible for mediating this behavioral effect but the ... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post... the journals themselves should be primarily responsible for storage. If datasets are too large to transfer...Improving access to data promotes dissemination, especially for young researchers who may be intimidated ... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...lentiviruses lack a functioning integrase, the enzyme responsible for inserting viral DNA into the host genome....unwanted interruptions. Insertional mutagenesis is especially concerning in clinical settings, as evidenced... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog Post...as FSC-A/SSC-A, FSC-H/SSC-H, or FSC-W/SSC-W, respectively. H, A, and W describe the shape of a histogram...population into DN, DP and SP (for each stain respectively). (B) Instead of a quadrant gate, I am free ... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog Post...targeted nucleases In an effort to combine the respective advantages of the AAV and CRISPR-based approaches...efficiencies in T-cells and hematopoietic stem cells respectively. Future researchers could apply this technique... -
Tips for Getting a Faculty Position
TypeBlog Post...Cell/Science paper. You do need multiple first/corresponding author publications in quality journals. It ... of guidance or the document examples are not especially helpful. I set out to make something practical... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post...using the online calculator from the Salis group) (Espah Borujeni et al., 2014). Replaced the pDS132 MCS ...bacteria become resistant to antibiotics! References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post...necessary for post-translational modifications. Despite these potential issues, GFP has been used successfully...complement a knock-down or knock-out of the corresponding protein, that is strong evidence that the fusion... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...likely it will contain random synthesis errors, especially single basepair deletions. Therefore, Gibson ...enzymes nick the DNA strand that contains their respective recognition site. This means that if the BbvCI... -
Evolution of Lab Techniques
TypeBlog Post...should ultimately contain radioactive bands that correspond to every nucleotide in your DNA sequence, based...Figure 2). Accuracy and accessibility led to the widespread use of Sanger sequencing. Building upon this ...