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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 637 results for: ESP

Showing: 321 - 340 of 637 results

  2. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...supports reproducibility RSpace was designed in response to requirements that emerged from intensive engagement...developed with these principles in mind, and in response to further input provided by the research communities...

  3. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...experimental target, SpCas9 and its enhanced variants, eSpCas9 and HypaCas9, are great choices. Check out our ... using a Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9 for site-specific...

  4. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...envelope protein on the virus’s surface and a corresponding receptor in the host cell. Some of these envelope-receptor...infecting neurons and cause minimal inflammatory response, but are not taken up by axon terminals and can...

  5. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...but like to be able to provide well thought out responses. I hung out with Joanne as she answered questions...toxic advisors, fruitless job searches, and even despair at having chosen an academic past. I hope I was...

  7. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...which protein fluors are likely to trigger immunoresponses. Nucleotides, in general, are far less immunogenic...small molecules that do not generate an immune response. Aptamers can be selected for these small ligands...

  8. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Gern, J., & Seroogy, C. (2017). Neonatal immune response to rhinovirus A16 has diminished dendritic cell...Greiner, D., & Anderson, D. (2015). Neutrophil Responses to Sterile Implant Materials. PloS One, 10, e0137550...

  9. Viral Production at Addgene

    Type
    Blog Post
    ...reported or posted on the material page for the corresponding catalog item (see maps section for images). ...targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease...

  12. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...mesothelioma many years later. While there is a dose-response relationship (the higher the exposure, the greater...cell, and binds cells that can begin an immune response. This important biomarker can be used to distinguish...

  13. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...targeted nucleases In an effort to combine the respective advantages of the AAV and CRISPR-based approaches...efficiencies in T-cells and hematopoietic stem cells respectively. Future researchers could apply this technique...

  14. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...lentiviruses lack a functioning integrase, the enzyme responsible for inserting viral DNA into the host genome....unwanted interruptions. Insertional mutagenesis is especially concerning in clinical settings, as evidenced...

  15. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...as FSC-A/SSC-A, FSC-H/SSC-H, or FSC-W/SSC-W, respectively. H, A, and W describe the shape of a histogram...population into DN, DP and SP (for each stain respectively). (B) Instead of a quadrant gate, I am free ...

  16. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...using the online calculator from the Salis group) (Espah Borujeni et al., 2014). Replaced the pDS132 MCS ...bacteria become resistant to antibiotics! References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard...

  18. Evolution of Lab Techniques

    Type
    Blog Post
    ...should ultimately contain radioactive bands that correspond to every nucleotide in your DNA sequence, based...Figure 2). Accuracy and accessibility led to the widespread use of Sanger sequencing. Building upon this ...

  20. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...Cell/Science paper. You do need multiple first/corresponding author publications in quality journals. It ... of guidance or the document examples are not especially helpful. I set out to make something practical...
Showing: 321 - 340 of 637 results