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...that has been validated in multiple assay types. Remember that ChIP uses native proteins (so antibodies ...extra-methodical approach" to the top of your protocols when embarking on your ChIP journey!  References and Resources...

...now, it’s time to cast aside those concerns and embrace a new resource to solve the problem: CasPEDIA, ...collection for plasmids containing that enzyme, and embedded in the References lists on every page you can ...

...rmmAkm6PQd — Elizabeth Kenkel (@ElizabethKenkel) December 5, 2018 How social media has helped me in science... researcher’s work day. It’s also important to remember that it’s not the amount of time you spend on ...

...pooled library In a paper published in Nature in December, Konermann and her colleagues in Feng Zhang’s ...was on the right track. The next insight was to assemble a synthetic transcription activation complex consisting...

...you give someone a card they are more likely to remember you if you follow up and if you give someone your... online to follow up with in person. Email lab members that have moved on to see if they will attend. ...

...use in stem cells. (See the complete protocol at StemBook). Addgene: How are you now using this tool? Musunuru...includes 834 plasmids for the pieces you need to assemble. Our system for CRISPR/Cas9 (also available from...

...proliferation of basal-like breast cancer cells. eLife. September 2017. 6: e26693. PubMed PMID: 28926338. PubMed...Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 2017. 171(7):1573-1588.e28 PubMed PMID: 29224777...

...between one heavy chain and one light chain. When assembled, the four chains make a Y-shaped protein that ...antib8020028    Wessel GM, McClay DR (1986) Two embryonic, tissue-specific molecules identified by a double-label...

.... Protein kinases deliver messages to the next members of their pathways, but where the kinases are located...nucleus. But if the inhibitor targeted upstream members of the pathway (Raf1, EGRF), the KTR localized ...

...Autophagosomes are relatively easy to identify: double-membraned structures containing undigested cytoplasmic contents...stands for protein) associates with autophagosomal membranes, so tagging it with GFP allows for tracking the...

...ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies, as ...resources and protocols! We invite feedback from all members of the research community to help us develop these...

...going to pour these plates now” to a fellow team member. But, at Addgene, we use seven different types ... professional life, let me know that, too. And remember, if you discover during grad school or during ...

..., the “Reproducibility for Everyone” team was assembled when it was realized that interactive workshops...connected, this diverse and international team assembled the right people at the right time. Be inclusive...

...and targeting abilities of the other Cas family members make for useful implements in the scientist toolbox...Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas. Molecular ...

... lab members. We can set ourselves up for success by giving explicit instructions to lab members through...

...something that is actually useful to you. When embarking on a new literature search, try to find a recent... rudimentary, but it works for me. I can often remember which paper I need to open, but when I can’t it...

...they’ve gone along, Voigt’s group has also been assembling the toolbox needed for anyone to begin considering...papers, published in Molecular Systems Biology in November, Voigt and Alex Nielsen describe what’s possible...

...targeted DNA modifications in transgenic animals, embryonic stem cells, and/or tissue-specific cell types:...oncogene only in a particular organ, or bypassing embryonic lethality. To facilitate the use of Cre-lox technology...

...days, weeks, or months. It is always helpful to remember that if a PI asks for assistance in the grant ...of the statements may be slightly far reaching, remember that you are writing a proposal to test hypotheses...

...Syn-driven, Cre-dependent Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby for labeling...constitutive Expression of mCherry-tagged, plasma membrane-tethered ibARK in astrocytes. 5 Jun Nagai Anterograde...